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Composition for tissue damage repair as well as preparation method and application thereof
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A tissue injury and composition technology, applied in the field of promoting tissue repair and/or regeneration, or reducing tissue fibrosis and repairing tissue damage, can solve problems such as the lack of effective means to improve tissue healing
Pending Publication Date: 2022-03-08
是光隽恒(北京)生物科技有限公司
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Embodiment 1
[0038] Embodiment 1: the preparation of the composition of the present application
[0039] Firstly, platelet-rich plasma is prepared, and the preparation method comprises the following steps:
[0040] 1) Draw 4ml of sodium citrate injection with a 50ml syringe in advance. Take 36ml of venous whole blood from the user who is pre-using this product, and mix it gently for anticoagulation;
[0041] 2) Dispense the mixture in step 1) into 15ml centrifuge tubes, 5ml in each tube;
[0043] 4) Absorb about 2ml of the upper plasma and platelet layer of each centrifuge tube, try not to absorb the red blood cell layer, transfer to another new 15ml centrifuge tube after aspiration, and merge the two into one, about 4ml;
[0044] 5) centrifuge the centrifuge tube in step 4) with 800g for 6min; and
[0045] 6) Absorb about 3 / 4 of the supernatant in step 5) and discard, and mix the remaining liquid to obtain platel...
[0049] About 200 g of Sprague-Dawley (SD) rats (purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.) were anesthetized with isoflurane, and intramuscularly injected cardiotoxin (CTX, 0.15 mg / ml, 50 μl , Sigmae-Aldrich) were injured. One day after CTX injection, the incision was reopened, and the CTX injury area was visible to the naked eye, see appendix figure 1 .
[0050] The modeling animals were randomly divided into 3 groups, 10 in each group. Group 1 was the control group, given PBS. Group 2 was administered platelet-rich plasma (PRP) prepared according to Example 1, and Group 3 was administered a mixture of PRP prepared according to Example 1 and TGFβ1 neutralizing antibody.
[0051] Inject 50 ml of PBS (control), PRP, or PRP and TGFβ1 neutralizing antibody mixture into the CTX-injured area. The incision is closed with subc...
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Abstract
The present application provides a composition for tissue damage repair comprising platelet-rich plasma and a substance that reduces the activity of transforming growth factor beta (TGF beta). The invention also provides a preparation method of the composition and application of the composition in preparation of drugs for promoting tissue repair and / or regeneration or for reducing fibrosis of damaged tissues.
Description
technical field [0001] The application belongs to the technical field of biomedicine, and in particular, the application relates to a composition for repairing tissue damage, a preparation method thereof, and the role of the composition in promoting tissue repair and / or regeneration, or reducing damaged tissue fibrosis use in . Background technique [0002] Tissue injury, especially muscle injury, is very common in sports medicine, but there is currently a lack of effective means to improve tissue healing. One of the main reasons is fibrosis at the injury site. Tissue damage repair, especially muscledamage repair, is a long-term challenge for both clinicians and basic research workers. [0003] Platelet-containing plasma (platelet rich plasma, PRP) is a biological agent / biological material derived from autologous whole blood that is being studied for soft tissue healing. It contains a large number of platelets, generally more than three times that of peripheral blood. Aft...
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