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Extraction method of roxburgh rose superoxide dismutase

A superoxide and extraction method technology, applied in the field of enzyme extraction, can solve the problems of low extraction rate, high cost, and organic solvent pollution, and achieve the effects of high activity, pollution avoidance, and efficient extraction

Pending Publication Date: 2022-03-08
西安全奥生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction methods of superoxide dismutase in Rosa roxburghii mainly use ultrasonic-assisted extraction, thermal denaturation method, isoelectric point method, organic solvent precipitation method, etc., and the common extraction methods have low extraction rate or are limited by organic solvent pollution and high cost. And other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] This example provides a test of a method for extracting superoxide dismutase from Rosa roxburghii.

[0021] 50g of Rosa roxburghii was pitted and pulverized, stirred at room temperature with 150mL 3% NaCl solution for 5h, filtered through a 100-mesh sieve, and the filtrate was centrifuged, and the centrifuged liquid was filtered through filter paper and decolorized with activated carbon to make a crude extract; After 30 minutes in a water bath at 55°C, quickly cool and filter to remove precipitated impurities, and take the first supernatant; use 3.5% NaOH solution to adjust the pH of the first supernatant to 5, stir and let it stand at room temperature for 15 minutes, then pour it into a centrifuge tube for After centrifugation, remove the precipitated impurities to obtain the second supernatant; add 10% volume of absolute ethanol to the second supernatant, let it stand at room temperature for 10 minutes, and centrifuge to get the supernatant, which is the purified solut...

Embodiment 2

[0023] This example provides a test of a method for extracting superoxide dismutase from Rosa roxburghii.

[0024] 50g of Rosa roxburghii was pitted and pulverized, stirred at room temperature with 200mL 3% NaCl solution for 5h, filtered through a 100-mesh sieve, and the filtrate was centrifuged, and the centrifuged liquid was filtered through filter paper and decolorized with activated carbon to make a crude extract; After 30 minutes in a water bath at 55°C, quickly cool and filter to remove precipitated impurities, and take the first supernatant; use 3.5% NaOH solution to adjust the pH of the first supernatant to 5, stir and let it stand at room temperature for 15 minutes, then pour it into a centrifuge tube for After centrifugation, remove the precipitated impurities to obtain the second supernatant; add 10% volume of absolute ethanol to the second supernatant, let it stand at room temperature for 10 minutes, and centrifuge to get the supernatant, which is the purified solut...

Embodiment 3

[0026] This example provides a test of a method for extracting superoxide dismutase from Rosa roxburghii.

[0027] 50g of Rosa roxburghii was pitted and pulverized, stirred at room temperature with 250mL 3% NaCl solution for 5h, filtered through a 100-mesh sieve, and the filtrate was centrifuged, and the centrifuged liquid was filtered through filter paper and decolorized with activated carbon to make a crude extract; After 30 minutes in a water bath at 55°C, quickly cool and filter to remove precipitated impurities, and take the first supernatant; use 3.5% NaOH solution to adjust the pH of the first supernatant to 5, stir and let it stand at room temperature for 15 minutes, then pour it into a centrifuge tube for After centrifugation, remove the precipitated impurities to obtain the second supernatant; add 10% volume of absolute ethanol to the second supernatant, let it stand at room temperature for 10 minutes, and centrifuge to get the supernatant, which is the purified solut...

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Abstract

The invention provides an extraction method of roxburgh rose superoxide dismutase, which comprises the following steps: denucleating roxburgh rose, crushing, mixing with an extraction salt solution, extracting, centrifugally filtering, and decolorizing with activated carbon to obtain a crude extract; precipitating the crude extract at the temperature of 50-55 DEG C and the pH value of 5.0, and adding 10% by volume of absolute ethyl alcohol for precipitating to obtain a purified solution; and carrying out reduced pressure vacuum concentration on the purified liquid at 45-50 DEG C until the concentration of the extracted salt is 20%, cooling, standing, centrifuging, washing and drying to obtain the superoxide dismutase. According to the extraction method of the roxburgh rose superoxide dismutase, the roxburgh rose raw material does not need to be subjected to special treatment, reagents used in the extraction process are safe, and the obtained superoxide dismutase is high in activity.

Description

technical field [0001] The invention belongs to the technical field of enzyme extraction, and relates to a method for extracting superoxide dismutase from roxburghii. Background technique [0002] Rosa roxbunghii (Rosa roxbunghii) is the fruit of the perennial deciduous shrub of Rosaceae. It is born in sunny hillsides, valleys, roadsides and bushes at an altitude of 500-2500 meters. Natural wild fruits in mountainous areas and other places are artificially planted on a large scale in Guizhou Province and Kaifeng City, Henan Province. Rosa roxburghii has good edible value and medicinal value. Prickly pear is rich in B vitamins, VC, flavonoids, organic acids and other components, which can protect the heart, relieve fatigue, enhance myocardial vitality, lower blood pressure, and enhance immunity. Strength, anti-aging, anti-cancer and other effects. Rosa roxburghii has a short harvest period and is the fruit with the highest superoxide dismutase content found so far. Therefor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02
CPCC12N9/0089C12Y115/01001
Inventor 王毅王蕊李言威
Owner 西安全奥生物科技有限公司
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