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Preparation method of konjak insect-proof fungicide

A technology of pest control and fungicide, which is applied in the field of preparation of konjac insect control fungicide, can solve the problems of high risk of use, weakened drug efficacy, high pesticide residues, etc., achieves easy preparation and mass production, solves difficult prevention and control, and has obvious effects Effect

Pending Publication Date: 2022-03-11
安康市农业科学研究院
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Problems solved by technology

[0004] The purpose of the present invention is just to provide a kind of preparation method of konjac pest control bacterial agent in order to solve the above-mentioned problem, solves that existing chemical agent phoxim, dichlorvos etc. commonly used for konjac pest control often cause high pesticide residues in the field, and dichlorvos is A highly toxic pesticide, the risk of use is extremely high. Beauveria bassiana and Metarhizium anisopliae biopesticides are often prepared in the form of spore powder + lubricant. Improper storage can easily lead to inactivation of the fungicide. Improper application will greatly weaken the efficacy and cause The disadvantages of konjac pests are difficult to prevent and control

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  • Preparation method of konjak insect-proof fungicide

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Embodiment Construction

[0022] Such as figure 1 Shown, this specific embodiment adopts following technical scheme: a kind of preparation method of konjac insect-proof fungicide, this preparation method comprises the following steps:

[0023] S1. Activation of Cordyceps militaris strains: use the basic medium formula to prepare a solid medium, and inoculate the preserved Cordyceps militaris strains on the solid medium for activation culture;

[0024] S2. Preparation of liquid strains: inoculating the above-mentioned activated Cordyceps militaris strains on a liquid medium for fermentation;

[0025] S3. Fermentation of liquid strains: placing the liquid strains prepared above on a constant temperature shaking incubator for cultivation;

[0026] S4, mycelia inactivation: the above-mentioned prepared liquid strains are placed in an autoclave to inactivate the mycelium of Cordyceps militaris, and then the liquid strains are filtered with a sieve to obtain the lysate bacterial liquid in Cordyceps militari...

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Abstract

The preparation method comprises the following steps: S1, activation of a cordyceps militaris strain: preparing a solid culture medium by utilizing a basic culture medium formula, and inoculating the preserved cordyceps militaris strain onto the solid culture medium for activation culture; s2, preparing a liquid strain: inoculating the activated cordyceps militaris strain on a liquid culture medium for fermentation culture; s3, liquid strain fermentation: putting the prepared liquid strain on a constant-temperature shaking incubator for culturing; the prepared liquid strain is placed in a high-pressure sterilization pot, after cordyceps militaris hyphae lose activity, the liquid strain is filtered through a sieve, the cordyceps militaris endolysate bacterium liquid can be obtained, and the konjak insect prevention bacterium liquid is prepared on the basis of the cordyceps militaris endolysate bacterium liquid. The composition has an obvious effect of preventing and controlling konjak pests, solves the problem that the konjak pests are difficult to prevent and control, and is easy to prepare and produce in batches.

Description

Technical field: [0001] The invention belongs to the technical field of konjac anti-insect fungicide, and in particular relates to a preparation method of konjac insect-proof fungicide. Background technique: [0002] As the conclusion that konjac pests are an important cause of disease occurrence is revealed, the research on konjac pest control has received more and more attention. Konjac pests are mainly divided into underground pests and aboveground pests. The underground pests are mainly grubs, cutworms, and needleworms, and the aboveground pests are mainly Lepidoptera hawkmoth larvae. Wounds caused by insect bites often become the best channel for pathogenic bacteria to invade. As one of the causes of disease, insect pests bring great risks to the field production of konjac. According to investigations, the commonly used agent for preventing and controlling konjac pests in production is phoxim. And dichlorvos, Cordyceps militaris, also known as Cordyceps militaris, is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCC12N1/14A01N63/30
Inventor 覃剑锋张园园段龙飞蔡阳光陈国爱张百忍刘次鹏杨凉花王宗方
Owner 安康市农业科学研究院