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Method for preparing structural lipid by enzyme method

A technology of enzymatic preparation and structured lipids, applied in the field of oils and fats, can solve the problems of high consumption of raw and auxiliary materials, low purity, high production costs, etc., and achieve the effect of increasing yield and overcoming high prices

Pending Publication Date: 2022-03-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of large consumption of raw and auxiliary materials, high production cost, low purity and complicated purification in the process of preparing UPU by directly reacting PPP with oleic acid and linoleic acid, the invention provides a method for preparing UPU with high purity and high yield

Method used

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  • Method for preparing structural lipid by enzyme method
  • Method for preparing structural lipid by enzyme method
  • Method for preparing structural lipid by enzyme method

Examples

Experimental program
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Effect test

Embodiment 1

[0037] The reaction was carried out in a reaction kettle, and 1 g of tripalmitin triglyceride was weighed and mixed with 4 g of ethanol, and 0.05 g of sn-1,3-specific lipase, namely Novozym 435, derived from Candida antarctica was added. Carry out the alcoholysis reaction at 200r / min, 50°C, and magnetically stir for 4h. After the reaction, lipase and impurities were removed by centrifugal filtration at a speed of 4000 r / min, and ethanol was removed by vacuum rotary evaporation at 40°C. Then add n-hexane (1:15) at 20°C for 1 hour to crystallize at a constant temperature. During the crystallization process, the water activity is controlled to be 0.1, and then the liquid part, such as fatty acid ethyl ester and other components with lower melting points, is removed, and the remaining solid part is sn- 2 palmitic acid monoglyceride, analyzed by HPLC-RID, sn-2 palmitic acid monoglyceride content is 73.03%.

[0038] According to the molar ratio of 1:12, take the above-mentioned sn-...

Embodiment 2

[0040] The reaction was carried out in a reaction kettle, and 1 g of tripalmitin triglyceride was weighed and mixed with 4 g of ethanol, and 0.1 g of sn-1,3-specific lipase, namely Novozym 435, derived from Candida antarctica was added. Carry out the alcoholysis reaction at 200r / min, 50°C, and magnetically stir for 4h. After the reaction, remove lipase and impurities by centrifugal filtration at a speed of 4000r / min, remove ethanol by vacuum rotary evaporation at 40°C, and then add n-hexane (1:15) at 20°C for 1 hour to crystallize, and control the water activity during the crystallization process 0.1, then remove the liquid part that is fatty acid ethyl ester and other components with lower melting points, the remaining solid part is the sn-2 palmitic acid monoglyceride, after HPLC-RID analysis, the sn-2 palmitic monoglyceride content is 78.51%.

[0041] According to the molar ratio of 1:12, take the above-mentioned sn-2 palmitic acid monoglyceride and the fatty acyl donor (i...

Embodiment 3

[0043] Step (1) is the same as Example 2, but the mol ratio of linoleic acid and oleic acid in step (2) is modified to 2:1, and all the other parameters are unchanged. As detected by GC, the relative content of sn-2 palmitic acid in the total palmitic acid was 66.81%; as detected by HPLC-ELSD, the content of UPU was 62.12%, see Table 2.

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Abstract

The invention discloses a method for preparing structural grease by an enzyme method, and belongs to the technical field of grease. The method comprises the following steps: firstly, carrying out alcoholysis reaction on raw oil and ethanol under the action of lipase, removing lipase and impurities after the reaction is finished, carrying out solvent crystallization to obtain sn-2-site palmitic acid-rich monoglyceride, weighing sn-2-site palmitic acid monoglyceride and a fatty acyl donor according to a molar ratio of 1: 3-1: 12, adding sn-1, 3 specific lipase, and carrying out hydrolysis reaction to obtain the sn-2-site palmitic acid-rich monoglyceride. And reacting the reaction system at the temperature of between 50 and 70 DEG C to obtain the UPU. The high-yield and high-purity UPU synthesis method provided by the invention avoids the defects of high raw material consumption, high production cost, multiple byproducts, complex purification and the like in the traditional UPU preparation process, and has certain development potential.

Description

technical field [0001] The invention belongs to the technical field of fats and oils, in particular to a method for enzymatically preparing structured fats. Background technique [0002] Breast milk has long been considered the ideal natural food for infants after birth. In the process of infant growth and development, breast milk plays a pivotal role and can provide almost all the nutrients needed by infants. Breast milk fat accounts for about 3% to 5% of breast milk, which can provide more than half of the energy for infants and young children. The triglyceride content in breast milk fat exceeds 98%. The fatty acid composition is mainly saturated fatty acids and monounsaturated fatty acids, of which sn-2 Most of the saturated fatty acids are saturated fatty acids, and the unsaturated fatty acids are mostly distributed in the sn-1,3 positions. Therefore, most of the triglycerides in breast milk exist in the form of 1,3-diunsaturated-2-saturated triglycerides (UPU), such as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/6445C12P7/6454C12P7/6472A23C9/152C11B3/00
CPCC12P7/6445C12P7/6454C12P7/6472A23C9/1528C11B3/00
Inventor 王小三黄卓能龚静茹王笑寒江聪黄雅祺
Owner JIANGNAN UNIV
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