Indoor weak structural plane reinforcing cementing fluid based on MICP and test method of indoor weak structural plane reinforcing cementing fluid
A weak structural surface, cementitious liquid technology, used in the application of stable shear force to test the strength of materials, the use of stable tension/pressure to test the strength of materials, and the preparation of samples for testing, etc. It can solve the problems of high cost and high cost, and achieve the effect of low cost, low risk and improvement of work status.
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Embodiment 1
[0045] A kind of indoor weak structural surface reinforcement test method based on MICP, mainly comprises the following steps:
[0046] (1) Prepare a liquid medium by mixing 10g / L peptone, 3g / L beef extract and 5g / L NaCl, adjust the pH to 7.0, autoclave at 121°C for 20min, and take it out to a sterile ultra-clean bench for use. On the basis of the composition of the liquid medium, add 20g of agar powder to each 1L of the medium, which will be in a liquid state after high-temperature sterilization, and put it into a sterile ultra-clean bench to cool to become a solid medium for use. Wipe the ampoule containing lyophilized powder of Bacillus pasteurianum with absorbent cotton soaked in 70% alcohol, heat its top near the flame of an alcohol lamp, and then drop a few drops of sterile water to the top of the heated ampoule to crack the glass, Gently tap the top of the cracked ampoule with tweezers. Use a sterile pipette to draw 1.0mL of liquid culture medium, drop it into the ampo...
Embodiment 2
[0058] Mechanical parameters of weak structural surfaces after MICP strengthening and Ca 2+ Exploration of Concentration Relationship
[0059] Due to Ca 2+ Direct participation of CaCO with urea 3 The precipitation process, so in the reinforcement test Ca 2+ The concentration of urea is also a key factor in determining the strengthening effect of weak structural surfaces. According to the chemical reaction (Formula 2), when Ca 2+ The reaction is the most complete when the concentration of urea is the same, so Ca 2+ Concentration and urea concentration were set at 1:1.
[0060]
[0061] Using deionized water as the blank control group, five different Ca 2+ Concentration (respectively 0mol / L, 0.25mol / L, 0.5mol / L, 1mol / L, 1.5mol / L) control test, test bacteria solution concentration OD 600 The value is 0.9, and the strengthening time of the specimen is 30d. Other methods are the same as before. Get the mechanical parameters and Ca 2+ Concentration curve.
[0062] The a...
Embodiment 3
[0065] Exploration on the relationship between mechanical parameters and strengthening time of weak structural surface after MICP strengthening
[0066] Six control experiments with different reinforcement times (5d, 10d, 15d, 20d, 25d, and 30d) were designed, and the concentration of the bacteria solution was OD 600 Value is 0.9, Ca 2+ The concentration and urea concentration are 0.5mol / L, and other methods are the same as before. The relationship curves of each mechanical parameter and strengthening time were obtained.
[0067] The analysis found that: during the period of 5-15 days, the bacteria need to adapt to the environment after a certain period of time, and then start to multiply and grow, the urease activity gradually increases, and the CaCO 3 As the formation rate increases, the strengthening effect on the weak interlayer and the cementation between the weak interlayer and the upper and lower rock blocks gradually increase, resulting in a gradual increase in the g...
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