Anti-tumor grifola frondosa glycoprotein as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of biological medicine, and discloses anti-tumor grifola frondosa glycoprotein as well as a preparation method and application thereof. The protein content of the anti-tumor grifola frondosa glycoprotein is 54.0 + / -3.0%, the polysaccharide content of the anti-tumor grifola frondosa glycoprotein is 20.3 + / -2.0%, the weight-average molecular weight of the anti-tumor grifola frondosa glycoprotein is 17.5 kDa, and the monosaccharide composition and the molar ratio of fucose to glucosamine hydrochloride to galactose to glucose to mannose to galacturonic acid to glucuronic acid are 2.4: 0.8: 3.9: 72.3: 7.7: 3.2: 9.6. The amino acids are composed of alanine, glycine, threonine, valine, leucine, isoleucine, proline, asparaginic acid, phenylalanine, tyrosine and glutamic acid. The grifola frondosa glycoprotein disclosed by the invention has the effects of enhancing immune response of a tumor body and inhibiting in-vivo growth of breast cancer through in-vivo verification.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an anti-tumor grifola glycoprotein and its preparation method and application. Background technique [0002] It is known that cancer originates from the gene mutation of autologous cells, which has strong genetic instability and heterogeneity. After decades of development, radiotherapy, chemotherapy, and surgery have made great progress, but in most cases, tumor growth cannot be completely controlled, and it is easy to lead to drug resistance and recurrence that is more difficult to treat. With the in-depth development and understanding of oncology and immunology, studies have found that the formation of tumors is the result of the body's immune escape. Due to the outstanding effects of immunosuppressive checkpoint inhibitors PD-1, PD-L1 and CTLA-4 antibodies in the treatment of advanced cancer, the 2018 Nobel Prize in Physiology or Medicine was awarded to tumor ...

AI Technical Summary

Problems solved by technology

The above published papers and public patents use strong acid-base and deproteinization processes in the process of extracting glycoproteins. The extracted active ingredients are different, and the anti-tumor effect is mainly to inhibit the proliferation of tumor cell lines in vitro at higher concentrations. Does not reflect whether it is effective in the body, and fails to verify the key components of its anti-tumor effect

This affects the further development and application of such products to a certain extent

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