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Anti-tumor grifola frondosa glycoprotein as well as preparation method and application thereof

A technique of Grifola frondosa and glycoprotein, which is applied in the field of biomedicine, can solve the problems such as the composition of key components that have not been verified for anti-tumor effect, the active components are different, and cannot reflect the effectiveness, and can inhibit growth in the body, increase the number, and enhance immunity. effect of response

Active Publication Date: 2022-03-25
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above published papers and public patents use strong acid-base and deproteinization processes in the process of extracting glycoproteins. The extracted active ingredients are different, and the anti-tumor effect is mainly to inhibit the proliferation of tumor cell lines in vitro at higher concentrations. Does not reflect whether it is effective in the body, and fails to verify the key components of its anti-tumor effect
This affects the further development and application of such products to a certain extent

Method used

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  • Anti-tumor grifola frondosa glycoprotein as well as preparation method and application thereof
  • Anti-tumor grifola frondosa glycoprotein as well as preparation method and application thereof
  • Anti-tumor grifola frondosa glycoprotein as well as preparation method and application thereof

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Embodiment 1

[0036] The preparation method of a kind of anti-tumor grifola glycoprotein of the present embodiment comprises the following preparation steps:

[0037](1) Dry the fruiting bodies of Grifola frondosa (purchased by Guangdong Yuewei Edible Fungi Technology Co., Ltd.) at 60°C to constant weight, superfine powder, pass through 100 mesh, take 200g of samples, soak them in 4.0L pure water for 3 hours, and heat at 100°C , reflux extraction twice, each time for 2 hours, combine the filtrate, vacuum concentrate the filtrate at 48-52°C to one-tenth of the original solution, centrifuge, remove insoluble matter, and obtain a crude concentrated solution.

[0038] (2) Mix the crude extract concentrate from step (1) with absolute ethanol at a ratio of 1:4, overnight at 4°C, and centrifuge (centrifugal force 8228g, 4°C, 15min) to obtain a precipitate. The precipitate was washed twice with pre-cooled absolute ethanol, centrifuged, the supernatant was removed, and the precipitate was obtained a...

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Abstract

The invention belongs to the technical field of biological medicine, and discloses anti-tumor grifola frondosa glycoprotein as well as a preparation method and application thereof. The protein content of the anti-tumor grifola frondosa glycoprotein is 54.0 + / -3.0%, the polysaccharide content of the anti-tumor grifola frondosa glycoprotein is 20.3 + / -2.0%, the weight-average molecular weight of the anti-tumor grifola frondosa glycoprotein is 17.5 kDa, and the monosaccharide composition and the molar ratio of fucose to glucosamine hydrochloride to galactose to glucose to mannose to galacturonic acid to glucuronic acid are 2.4: 0.8: 3.9: 72.3: 7.7: 3.2: 9.6. The amino acids are composed of alanine, glycine, threonine, valine, leucine, isoleucine, proline, asparaginic acid, phenylalanine, tyrosine and glutamic acid. The grifola frondosa glycoprotein disclosed by the invention has the effects of enhancing immune response of a tumor body and inhibiting in-vivo growth of breast cancer through in-vivo verification.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an anti-tumor grifola glycoprotein and its preparation method and application. Background technique [0002] It is known that cancer originates from the gene mutation of autologous cells, which has strong genetic instability and heterogeneity. After decades of development, radiotherapy, chemotherapy, and surgery have made great progress, but in most cases, tumor growth cannot be completely controlled, and it is easy to lead to drug resistance and recurrence that is more difficult to treat. With the in-depth development and understanding of oncology and immunology, studies have found that the formation of tumors is the result of the body's immune escape. Due to the outstanding effects of immunosuppressive checkpoint inhibitors PD-1, PD-L1 and CTLA-4 antibodies in the treatment of advanced cancer, the 2018 Nobel Prize in Physiology or Medicine was awarded to tumor ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/37C07K1/14C07K1/34C07K1/18C07K1/36A61K38/16A61K9/00A61P35/00A61P37/04A23L33/195
CPCC07K14/37A61K9/0053A61K9/0019A61P35/00A61P37/04A23L33/195A61K38/00A23V2002/00A23V2200/308A23V2200/324A23V2250/546
Inventor 李向敏阮勤钊吴清平谢意珍胡惠萍田回香刘远超肖春陈少丹雍天乔高雄梁晓薇蔡曼君吴晓贤
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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