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Bionic construction method of periosteum-like tissue

A construction method and technology of membrane tissue, applied in the intersecting fields of materials, life and medicine, can solve problems such as failure to meet critical defect repair needs, complex fabrication, low biological activity, etc.

Pending Publication Date: 2022-03-29
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a variety of periosteum-imitating materials based on organic or composite materials have been developed, but most of the periosteum-imitating materials have defects such as complicated production and insufficient osteogenic differentiation ability, and have no or only low biological activity. / Ineffective treatment of cartilage defects or avascular osteonecrosis, especially in elderly patients
However, the source of autologous periosteum tissue is limited, and it cannot meet the repair needs of critical defects.

Method used

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  • Bionic construction method of periosteum-like tissue
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  • Bionic construction method of periosteum-like tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Preparation of implant material

[0066] Add 10 μg of recombinant human bone morphogenetic protein-7 (rhBMP-7) synthesized by eukaryotic or prokaryotic expression system to gelatin sponge (5 mm diameter × 5 mm thick, 10 mg weight), and freeze-dry to form an active material containing BMP-7.

[0067] Add 30 μg of recombinant human bone morphogenetic protein-2 (rhBMP-2) synthesized by eukaryotic or prokaryotic expression system into gelatin sponge (5mm diameter×5mm thickness, 10mg weight), and freeze-dry to form the active material containing BMP-2.

[0068] Add 30 μg rhBMP-2 and 100ng vascular endothelial growth factor (VEGF) synthesized by eukaryotic or prokaryotic expression system to gelatin sponge (5mm diameter×5mm thickness, 10mg weight), and freeze-dry to form the active material containing BMP-2 / VEGF .

[0069] 30 μg of rhBMP-2 synthesized by eukaryotic or prokaryotic expression system and 30 μg of chondroitin sulfate (CS) were added to gelatin sponge (5mm diamet...

Embodiment 2

[0071] In vivo construction of periosteoid tissue in mice

[0072] The periostoid tissue was formed in mice using the active material containing BMP-7 or BMP-2 or BMP-2 / VEGF or BMP-2 / CS described in Example 1. Such as figure 1 As indicated, the above materials were implanted subcutaneously in the back of 8-week-old C57BL / 6 male mice. The active material formed after implantation in mice developed into periosteoid tissue after 1 week. After feeding for 1 week, the constructed periostoid tissue was taken out, part of which was used to take macroscopic photos, tissue sections, etc. for the characterization of periostoid tissue, and the other part was used for autologous skull defect transplantation.

[0073] figure 2 H&E section of the periosteum tissue surrounding the native femur and skull, showing the microstructure of the femur and lateral skull in situ.

[0074] image 3 The shown hematoxylin / eosin (H&E) stained sections showed that: the periosteoid tissue formed in mi...

Embodiment 3

[0079] In vivo construction of periosteoid tissue for autologous calvarial defect therapy in mice

[0080] The purpose of this example is to evaluate the therapeutic effect of periostoid tissue produced in mice on autologous calvarial defects with a diameter of 5 mm.

[0081] grouping:

[0082] SPF grade C57BL / 6 mice, male, 8 weeks old, were randomly divided into groups. The experimental groups are as follows:

[0083] group blank group BMP-2 / CS group quantity 6 6

[0084] Preparation of periostoid tissue: The scaffold containing BMP-2 / CS described in Example 1 was implanted subcutaneously, and the periostoid tissue was produced after 1 week of development. The periostoid tissue was removed, and the resulting disc-shaped periostoid tissue with a diameter of 5 mm was trimmed using a 5 mm inner diameter punch.

[0085] Autologous periosteoid tissue transplantation: After the mice were anesthetized, the skin of the mouse head was cut open with a scalp...

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Abstract

The invention relates to a bionic construction method of periosteum-like tissues. The bionic construction method comprises the step of implanting a biological material loaded with active substances and / or cells into an animal or human body to generate the periosteum-like tissues after development. The constructed periosteum-like tissue has a typical fibrous structure, contains abundant periosteum-derived stem cells and functional blood vessels, can repair bone defects with critical dimensions, and is expected to be applied to clinical treatment of severe bone / cartilage defects or old patients with weak regeneration capacity and the like.

Description

technical field [0001] The invention belongs to the cross field of materials, life and medicine, and relates to a novel bionic construction method of periosteum tissue. The periosteum tissue is constructed by activating the regeneration ability of the organism itself through active biological materials, which can treat bone / skeletal tissue caused by disease or trauma. Cartilage defect or deformity. Background technique [0002] As a fibrous tissue covering the surface of the main long bones in the human body, the periosteum is rich in mesenchymal stem cells (MSCs), blood vessels and nerve endings, and is crucial for bone development and post-traumatic repair. The development of periosteum-like materials to treat autologous bone / cartilage defects has become an effective new strategy. At present, a variety of periosteum-imitating materials based on organic or composite materials have been developed, but most of the periosteum-imitating materials have defects such as complicat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/38
CPCA61L27/3834A61L27/3821A61L27/3847A61L27/3852A61L27/3604A61L27/3608A61L27/3625A61L2430/02A61L2430/06A61L2430/40
Inventor 刘昌胜戴凯王靖俞远满邓顺书朱富威
Owner EAST CHINA UNIV OF SCI & TECH
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