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Ginkgo biloba extract for treating osteoarthritis

A technology for osteoarthritis and bone joints, which is applied in the medical field to achieve the effect of protecting joints

Pending Publication Date: 2022-04-05
THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In short, the current clinical treatment of arthritis still has obvious limitations, and new drugs are urgently needed to improve the clinical symptoms of arthritis patients and improve the long-term quality of life

Method used

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  • Ginkgo biloba extract for treating osteoarthritis
  • Ginkgo biloba extract for treating osteoarthritis
  • Ginkgo biloba extract for treating osteoarthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Ginkgolides Improve Cartilage Extracellular Matrix Synthesis Ability

[0025] Fetal mouse chondrocytes were isolated and cultured, and the cells were cultured in DMEM / F12 medium, and passaged when the density reached 80%-90%. The P1 generation chondrocytes were 3000 cells / cm 2 Inoculate in 6-well / 12-well / 24-well cell culture plate and culture in DMEM / F12 medium. RT-PCR, WB and immunofluorescence were used to detect the effects of ginkgolides on the synthesis and metabolism of chondrocytes. The specific groups are: control group, interleukin group, interleukin + ginkgolide group. After culturing for 5 days, immunofluorescence staining, RT-PCR detection and protein gel electrophoresis detection were carried out respectively. MAIN OUTCOME MEASURES: Expression of type II collagen and glycosaminoglycans. The result is as figure 1 As shown, the intervention of ginkgolide in vitro can promote the synthesis of cartilage extracellular matrix.

[0026] Ginkgolide ...

Embodiment 2

[0027] Example 2 Ginkgolides Inhibit Cartilage Extracellular Matrix Degradation Ability

[0028] The P1 generation chondrocytes were 3000 cells / cm 2 Inoculate in 6-well / 12-well / 24-well cell culture plate and culture in DMEM / F12 medium. RT-PCR, WB and immunofluorescence were used to detect the effects of ginkgolides on the synthesis and metabolism of chondrocytes. The specific groups are: control group, interleukin group, interleukin + ginkgolide group. After culturing for 5 days, immunofluorescence staining, RT-PCR detection and protein gel electrophoresis detection were carried out respectively. MAIN OUTCOME MEASURES: Expression of MMP13 (matrix metalloproteinase) and ADAMTS5 (proteoglycanase). The result is as figure 2 As shown, in vitro ginkgolide intervention can inhibit the degradation of cartilage extracellular matrix.

Embodiment 3

[0029] Example 3 Application of Ginkgolide in vivo to Improve and Delay Articular Cartilage Degeneration

[0030] (1) C57 / BL6 mice were selected as experimental animals and divided into: control group, arthritis group, arthritis + ginkgolide group. The mouse arthritis model was achieved by classic medial meniscus destabilization (DMM). Intraperitoneal injections were performed one week after the operation, twice a week. Knee joint samples were collected at the eighth week after the operation for follow-up testing.

[0031] (2) Imaging evaluation of the effect of ginkgolides on subchondral bone sclerosis. The specific implementation steps were as follows: the mouse knee joint was fixed in formalin for 48 h. Afterwards, μCT equipment was used for scanning, and the region of interest was the medial tibial platform. Quantitative analysis was performed using analysis software and a 3D reconstruction platform. The result is as image 3 As shown, ginkgolides inhibit abnormal hard...

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Abstract

The invention belongs to the technical field of medicines, and develops and verifies a ginkgo biloba extract for treating osteoarthritis. Through early-stage bioinformatics screening, it is predicted that bilobalide serving as an extract monomer of traditional Chinese medicine ginkgo biloba has a potential anti-arthritis function. In-vitro experiments intervene human cartilage cells in an arthritis environment through ginkgolides with different concentrations, and the steady-state change of an extracellular matrix of the cartilage cells is detected; in-vivo experiments are performed through intraperitoneal injection of bilobalide, and the influence of bilobalide treatment on the steady state of cartilage of a wounded mouse is observed. The bilobalide obtained by the invention has a strong protection effect on cartilage metabolism balance. An in-vivo animal model further verifies that bilobalide can relieve articular cartilage degeneration after injury. New theoretical and experimental references are provided for research on treatment of osteoarthritis by the traditional Chinese medicine monomers at present, the defect that the existing osteoarthritis treatment effect is poor is overcome, and the clinical treatment prospect of the traditional Chinese medicine monomers in osteoarthritis is widened.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a ginkgo extract used for treating osteoarthritis. Background technique [0002] Osteoarthritis is a serious bone and joint degenerative disease under the background of the current aging population. With the increase of life expectancy, the huge harm caused by osteoarthritis has become increasingly serious. For early-stage arthritis patients, interventions are mostly limited to oral analgesics to relieve symptoms; for late-stage severe arthritis patients, in order to maintain normal joint mobility, often only surgical intervention can be used. In short, the current clinical treatment of arthritis still has obvious limitations, and new drugs are urgently needed to improve the clinical symptoms of arthritis patients and improve the long-term quality of life. [0003] Ginkgo biloba, as a fast-growing and precious wood species, has light yellow sapwood, light yellowish brown heartw...

Claims

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Application Information

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IPC IPC(8): A61K31/365A61K36/16A61P19/02A61P29/00
Inventor 赵志坚张一健朱雪松何帆刘滔杨惠林
Owner THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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