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Preparation of biological cathode BOD (Biochemical Oxygen Demand) sensing system based on autotrophic and heterotrophic conversion

A sensing system and biocathode technology, applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problems that restrict the preparation of biocathode sensors, achieve rapid detection and overcome the effect of low sensitivity

Pending Publication Date: 2022-04-05
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult for such aerobic autotrophic microorganisms to quickly form a stable biofilm in a short period of time, which greatly restricts the preparation of biocathode sensors.

Method used

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  • Preparation of biological cathode BOD (Biochemical Oxygen Demand) sensing system based on autotrophic and heterotrophic conversion
  • Preparation of biological cathode BOD (Biochemical Oxygen Demand) sensing system based on autotrophic and heterotrophic conversion
  • Preparation of biological cathode BOD (Biochemical Oxygen Demand) sensing system based on autotrophic and heterotrophic conversion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0027] Example 1: Response of Biocathode BOD Sensor to Glucose

[0028] 1) Construction of biocathode BOD sensor

[0029] Acinetobacter-378 was modified onto the graphite sheet working electrode as mentioned above. The prepared catholyte was added to the MEC reactor and the circulating liquid reserve bottle, and the circulating liquid reserve bottle was continuously aerated to provide inorganic carbon source and electron donor. Start the peristaltic pump and feed the entire system in recirculation mode. The three electrodes are connected to an eight-channel electrochemical system respectively, and the chronoamperometric real-time monitoring of the biofilm is carried out at a constant potential of -0.2V, such as figure 1 shown.

[0030] 2) Response of biocathode BOD sensor to different concentrations of glucose

[0031] When the biocathode BOD sensor reached a stable baseline current for 12 hours, it was tested for the shock response of organic matter. In order to simulate...

Embodiment example 2

[0032] Example 2: Response of biocathode BOD sensor to sodium acetate

[0033] 1) Construction of biocathode BOD sensor

[0034]Acinetobacter-378 was modified onto the graphite sheet working electrode as mentioned above. The prepared catholyte was added to the MEC reactor and the circulating liquid reserve bottle, and the circulating liquid reserve bottle was continuously aerated to provide inorganic carbon source and electron donor. Start the peristaltic pump to circulate the entire system. The three electrodes are connected to an eight-channel electrochemical system respectively, and the chronoamperometric real-time monitoring of the biofilm is carried out at a constant potential of -0.2V, such as figure 1 shown.

[0035] 2) Response of biocathode BOD sensor to different concentrations of sodium acetate

[0036] When the biocathode BOD sensor reached a stable baseline current for 12 hours, it was tested for the shock response of organic matter. In order to simulate the ...

Embodiment 3

[0037] Example 3: Response of biocathode BOD sensor to combined shock of glucose and sodium acetate

[0038] 1) Construction of biocathode BOD sensor

[0039] Acinetobacter-378 was modified onto the graphite sheet working electrode as mentioned above. The prepared catholyte was added to the MEC reactor and the circulating liquid reserve bottle, and the circulating liquid reserve bottle was continuously aerated to provide inorganic carbon source and electron donor. Start the peristaltic pump to circulate the entire system. The three electrodes are connected to an eight-channel electrochemical system respectively, and the chronoamperometric real-time monitoring of the biofilm is carried out at a constant potential of -0.2V, such as figure 1 shown.

[0040] 2) Response of biocathode BOD sensor to the combined shock of different concentrations of glucose and sodium acetate

[0041] When the biocathode BOD sensor reached a stable baseline current for 12 hours, it was subjected ...

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Abstract

The invention discloses a preparation method and application of an artificial cathode biofilm BOD (Biochemical Oxygen Demand) sensor for monitoring biochemical oxygen demand in real time. According to the sensing system, a cathode sensing element is constructed by selecting a cathode characteristic bacterium Acinetobacter-378 which is passaged and purified for a long time by a research group and has electroautotrophic and heterotrophic functions. The Nafion / PTFE embedding medium is utilized to artificially manufacture the biological membrane, so that the problem that cathode microorganisms are difficult to quickly form a stable biological membrane in a short time is solved. The current response is fitted through response to impacts of glucose, sodium acetate and the like with different concentrations, so that quick, sensitive and low-cost early warning and real-time monitoring on the BOD in the aerobic water body are realized.

Description

technical field [0001] The invention relates to the field of microbial electrochemistry, in particular to a method for preparing an artificial biofilm and a method for measuring biochemical oxygen demand (BOD). Background technique [0002] Biochemical oxygen demand (BOD) is a comprehensive index used to reflect the content of degradable organic pollutants in wastewater. The determination of BOD is of great significance to the early warning and control of water pollution. Traditional BOD monitoring and early warning mainly use physical and chemical analysis methods, such as dissolved oxygen electrode method, photometric method, differential pressure method, etc. Although some of the test methods have been approved and have reached marketization, manual sampling and 5-day testing cannot be avoided. Limitation of various issues such as the length of the test and the inability to achieve real-time water quality monitoring. Compared with traditional physical and chemical analys...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N27/30G01N27/327
Inventor 王鑫廖承美韩沂琏李楠
Owner NANKAI UNIV
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