Folium artemisiae argyi essential oil nano preparation as well as preparation method and application thereof
A technology for essential oils and preparations of argyi leaves, applied in the field of medicine, can solve the problems of low solubility, low stability of natural plant essential oils, poor antibacterial effect, etc., and achieve the effects of reducing bacterial content, good application prospect and good antibacterial effect.
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Embodiment 1
[0032] The present invention adopts saturated absolute ethanol solution method and decompression rotary steaming technology to prepare natural plant essential oil-hydroxypropyl-β-cyclodextrin nano-preparation, and the preparation process of the present invention is as follows figure 1 shown.
[0033] The preparation method is: add 10g of hydroxypropyl-β-cyclodextrin into the absolute ethanol solution to prepare the hydroxypropyl-β-cyclodextrin solution; slowly add 5g of Artemisia argyi essential oil into the above solution, and stir at a constant temperature of 45°C for 3h , the solution was evaporated by a rotary evaporator, and dried in an oven at 60°C.
[0034] Take mugwort essential oil, mugwort essential oil-hydroxypropyl-β-cyclodextrin nano-preparation, hydroxypropyl-β-cyclodextrin, and detect its absorbance in the range of 200-800 wavelengths, figure 2 It is the full-wavelength scanning image of Artemisia argyi essential oil (1), Artemisia argyi essential oil-hydroxyp...
Embodiment 2
[0037] Set the mass ratio of mugwort essential oil: hydroxypropyl-β-cyclodextrin to 1:10, 1:8, 1:6, 1:4, 1:2.5, 1:2, 1:1.5, 1:1; The temperature was set at 75°C, 60°C, and 45°C; the reaction time was set at 1h, 3h, and 5h.
[0038] as the picture shows, Figure 4a -c is the result graph of the influence of temperature and time on the encapsulation efficiency, drug loading and yield of Folium Artemisiae Argyi essential oil-hydroxypropyl-β-cyclodextrin, Figure 5a -b is the diagram of the effect of different mass ratios of Artemisia argyi essential oil: hydroxypropyl-β-cyclodextrin on the encapsulation efficiency and drug loading of nano-preparations. The results showed that when the mass ratio of Artemisia argyi essential oil: hydroxypropyl-β-cyclodextrin was 1:2-2.5, the reaction temperature was 45°C, and the reaction time was 3 hours, when the drug loading was used as the main index, the Artemisia argyi at this time The drug loading, encapsulation efficiency and yield of es...
Embodiment 3
[0040] The nano-preparation obtained in Example 2 is used for space antibacterial research.
[0041]The nano-preparation of Artemisia argyi leaf essential oil of the present invention is put into a humidifier, and the nano-inclusion complex dissolves rapidly in the water tank of the humidifier, and the oscillator is used to drive the atomizing vibrator to generate resonance to form an ultrasonic wave, and according to the micro-shock wave theory and surface tension wave theory, the water solution is generated. Atomization, under the impetus of the fan, the atomized nano-preparation is sent to the air through the pipeline along with the water mist. After entering the air, the plant essential oil in the preparation "escapes" into the air immediately, achieving a good effect of antibacterial and disinfection in the air.
[0042] Control group: space 17-18m 3 After fumigation with distilled water for 3 hours, the number of colonies in the air was collected by the microbial sample...
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