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Active scaffold for multi-cell printing and nervating bone regeneration as well as preparation method and application of active scaffold

A bone regeneration and multicellular technology, which is applied in manufacturing, prosthesis, additive processing, etc., can solve the problems of poor bone regeneration and neglect of the important role of the nervous system

Active Publication Date: 2022-04-12
SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current 3D printed bone tissue engineering scaffolds ignore the important role of the nervous system in the bone repair process, resulting in poor bone regeneration.

Method used

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  • Active scaffold for multi-cell printing and nervating bone regeneration as well as preparation method and application of active scaffold
  • Active scaffold for multi-cell printing and nervating bone regeneration as well as preparation method and application of active scaffold
  • Active scaffold for multi-cell printing and nervating bone regeneration as well as preparation method and application of active scaffold

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0055] Preparation of bio-ink: first prepare the concentrated solution of bone marrow mesenchymal stem cells, and then mix it uniformly with the above-mentioned hydrogel slurry to obtain bio-ink A with a cell density of 3,000,000 cells / mL. Similarly, the concentrated solution of Schwann cells was prepared, and then evenly mixed with the above-mentioned hydrogel slurry to obtain bioink B with a cell density of 3,000,000 cells / mL.

[0056] Printing process: Bio-inks A and B were printed using two pneumatic extrusion needles 1 and 2, respectively. First use needle 1 to extrude bio-ink A to print several layers as the lower layer, then use needle 2 to extrude bio-ink B to print several layers as the upper layer, the ratio of the number of upper and lower layers is 5:1-1:5, more preferably 1:1. In addition, the pressure of the two extrusion needles is 20-100 Kpa, the temperature of the extrusion needles is controlled at 18-22°C, and the temperature of the deposition table is contro...

Embodiment 1

[0060] Effects of calcium silicate nanowires on the proliferation, migration and differentiation of bone marrow mesenchymal stem cells and Schwann cells:

[0061] Firstly, calcium silicate nanowires were prepared by hydrothermal method, and 9.446g of Ca(NO 3 ) 2 4H 2 O and 11.368 g of Na 2 SiO 3 9H 2 O was dissolved in 100 mL of deionized water, and magnetically stirred until completely dissolved. Na 2 SiO 3 9H 2 O solution was poured into Ca(NO 3 ) 2 4H 2 In the O solution, magnetically stir for 1 hour to form a uniformly dispersed white suspension; then put it into a hydrothermal kettle for 24 hours of hydrothermal reaction at 200 ° C, after cooling, filter it with suction and wash it thoroughly, you can get a good shape calcium silicate nanowires.

[0062] figure 1 The scanning electron microscope and X-ray diffraction results of the calcium silicate nanowires prepared according to the above method indicate that the calcium silicate nanowire material has been s...

Embodiment 2

[0072] Preparation and characterization of composite hydrogel scaffolds with gradient concentrations of calcium silicate nanowires: firstly, hydrogel slurries containing different calcium silicate nanowire contents were prepared. First weigh 0.025g of LAP photoinitiator and dissolve it in 10mL of deionized water, then add 0.6g of methacrylated gelatin, fully dissolve at 60°C to obtain a 6wt% methacrylated gelatin solution. After drawing 3mL of methacrylylated gelatin solution and mixing evenly with 0.0036g of calcium silicate nanowires, a hydrogel slurry containing 2% calcium silicate nanowires can be obtained; similarly, drawing 3mL of methacrylic After uniformly mixing the acylated gelatin solution with 0.0072 g of calcium silicate nanowires, a hydrogel slurry containing 4% calcium silicate nanowires can be obtained.

[0073] The effect of incorporation of different contents of calcium silicate nanowires on the rheological properties of methacrylated gelatin hydrogels was fi...

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Abstract

The invention relates to an active scaffold for multi-cell printing and nervating bone regeneration as well as a preparation method and application of the active scaffold. The multi-cell printing nervous bone regeneration active scaffold is a multi-cell printing nervous bone regeneration active scaffold containing nerve cells. The multi-cell printing nervous bone regeneration active scaffold has an upper / lower layer structure, wherein the upper layer structure is at least one layer containing a bioactive inorganic material; the upper layer structure comprises an organic hydrogel material with good biocompatibility and an upper layer support of nerve-related cells, and the lower layer structure comprises at least one layer of lower layer support comprising a bioactive inorganic material, the organic hydrogel material with good biocompatibility and bone-related cells.

Description

technical field [0001] The invention relates to a preparation method and application of a multi-cell printing active scaffold for neuralized bone regeneration, the scaffold can effectively promote the directional differentiation of nerve cells and bone-related cells, and is a potential treatment method for realizing neuralized bone regeneration. At the same time, a preparation method of the above-mentioned scaffold is provided, and a variety of cells can maintain high survival rate, good spreading and adhesion and excellent anisotropic differentiation ability on the scaffold. It belongs to the field of biotechnology. Background technique [0002] Bone is a multifunctional organ with complex components and structures. For example, a large number of sensory nerve fibers and sympathetic nerve fibers are distributed inside the bone. They actively participate in the growth, development and metabolism of bones by secreting a variety of neurotransmitters and neurotrophic factors. ...

Claims

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Application Information

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IPC IPC(8): A61L27/10A61L27/12A61L27/20A61L27/22A61L27/24A61L27/38A61L27/52A61L27/58B29C64/106B33Y10/00B33Y70/10B33Y80/00
Inventor 吴成铁张洪健
Owner SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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