Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method and application of sea cucumber polysaccharide

A technology of sea cucumber polysaccharides and sea cucumbers, which is applied in the field of sea cucumber polysaccharides and its preparation, can solve the problems of not conforming to the green sustainable economic development model, high energy consumption, and extensive extraction process, so as to improve the purity of polysaccharides, improve the service life, and ensure repeated sexual effect

Pending Publication Date: 2022-04-12
MUDANJIANG YOUBO PHARMA CO LTD +2
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the above method will use a large amount of fresh water and acid-base salts in the extraction process, it is a high-energy-consuming industry, and the acid-base salts discharged will have a certain ecological impact on the surrounding environment, which does not conform to the green sustainable economic development model, and the extraction process relatively extensive

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of sea cucumber polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of embodiment 1 sea cucumber polysaccharide

[0027] 1) Extraction of crude sea cucumber polysaccharide: Weigh 3 kg of dry rough sea cucumber, crush it into coarse particles, add pure water 8 times the weight of sea cucumber, and enzymolyze it with papain. pH 6.0, temperature 50°C, time 6h; after enzymolysis, boil to inactivate the enzyme, centrifuge at 15000rpm in a tube centrifuge, take the supernatant, concentrate under reduced pressure (temperature 50°C, vacuum ≤ -0.085MPa) to the original 20% of the volume, again tube centrifuge 15000rpm centrifugal, get the supernatant; supernatant salting out alcohol precipitation for the first time: add potassium acetate (making potassium acetate final concentration be 0.5M) in the supernatant, and ethanol ( Make the final concentration of ethanol be 60%), centrifuge to get the precipitate in a tube centrifuge; dissolve the precipitate with an appropriate amount of pure water, and carry out the second salting-out...

Embodiment 2

[0030] The preparation of embodiment 2 sea cucumber polysaccharides

[0031] 1) Extraction of crude sea cucumber polysaccharide: Weigh 3 kg of dry rough sea cucumber, crush it into coarse particles, add pure water 10 times the weight of the sea cucumber, and enzymolyze it with papain. pH 7.0, temperature 55°C, time 5h; after enzymolysis, boil to inactivate the enzyme, centrifuge at 12000rpm in a tube centrifuge, take the supernatant, concentrate under reduced pressure (temperature 50°C, vacuum ≤ -0.085MPa) to the original 25% of the volume, centrifuged again at 16000rpm in a tubular centrifuge for 10-15min, and got the supernatant; ), and ethanol (making the final concentration of ethanol be 60%), the tube centrifuge is centrifuged to get the precipitate; Concentration is 1.0M), and ethanol (making ethanol final concentration be 40%), tube type centrifuge is centrifuged to get precipitation; Precipitation is dissolved with appropriate amount of pure water, carry out the third...

Embodiment 3

[0034] The preparation of embodiment 3 sea cucumber polysaccharides

[0035] 1) Extraction of crude sea cucumber polysaccharide: Weigh 3 kg of dry rough sea cucumber, crush it into coarse particles, add pure water 12 times the weight of sea cucumber, and enzymolyze it with papain. pH 6.5, temperature 60°C, time 6h; after enzymolysis, boil to inactivate the enzyme, centrifuge at 14000rpm in a tube centrifuge, take the supernatant, concentrate under reduced pressure (temperature 50°C, vacuum ≤ -0.085MPa) to the original 20% of the volume, again tube centrifuge 14000rpm centrifugal, get the supernatant; supernatant salting out alcohol precipitation for the first time: add potassium acetate (making potassium acetate final concentration be 0.5M) in the supernatant, and ethanol ( Make the final concentration of ethanol be 60%), centrifuge to get the precipitate in a tube centrifuge; dissolve the precipitate with an appropriate amount of pure water, and carry out the second salting-o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a sea cucumber polysaccharide preparation method, which comprises: (1) taking dry sea cucumber, crushing into coarse particles, adding water, carrying out enzymolysis, carrying out centrifugation to obtain a supernatant, carrying out reduced pressure concentration on the supernatant to 1 / 5 of the original volume, and carrying out centrifugation under the same centrifugation condition to obtain a supernatant; performing salting-out and alcohol precipitation on the supernate for three times, washing the precipitate twice with absolute ethyl alcohol, and volatilizing the ethyl alcohol to obtain a sea cucumber polysaccharide crude product; (2) dissolving the sea cucumber polysaccharide crude product with pure water, loading the dissolved sea cucumber polysaccharide crude product on an anion chromatographic column (OH <->), eluting with pure water and a NaCl solution with gradient concentration, and collecting a NaCl eluent; and (3) carrying out ultrafiltration desalination on the NaCl eluent, concentrating the ultrafiltration trapped fluid under reduced pressure, and freeze-drying to obtain the sea cucumber polysaccharide. The sea cucumber polysaccharide can be used for preparing drugs or health care products for resisting hyperlipidemia, resisting oxidation, resisting blood coagulation, resisting tumors, improving immunity and the like, has the effect of preventing thrombus, and has a good auxiliary prevention effect on old people and people with high risk of thrombus.

Description

technical field [0001] The invention relates to a sea cucumber polysaccharide and its preparation method and application. [0002] technical background [0003] Sea cucumber is a traditional nourishing Chinese medicinal material. The efficacy of sea cucumber is highly recognized. However, the processing method and consumption method are simple and single, the absorption effect cannot be guaranteed, and it is easy to cause waste of nutrients. [0004] Sea cucumber is a traditional health product in my country, which has extremely high health care and nutritional value, and is also an important research object of marine functional food. Sea cucumber polysaccharides, as one of the important active ingredients of sea cucumbers, have various biological activities such as anti-hyperlipidemia, anti-oxidation, anti-coagulation, anti-tumor, and enhancing immunity. The research on sea cucumber polysaccharides mainly focuses on the body wall of sea cucumbers, which include sea cucumber...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L33/125A61K31/715A61P3/06A61P39/06A61P7/04A61P35/00A61P37/04
Inventor 李振国郑顺亮李伟胡杰文南志远周剑波郭予茜李长江张凯夏珂
Owner MUDANJIANG YOUBO PHARMA CO LTD