Method for detecting glyphosate
A technology of glyphosate and linear equations, applied in the field of glyphosate detection, can solve the problems of expensive instruments, high cost, and limited popularization and use
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Embodiment 1
[0045] Preparation of cerium ion-pyrophosphate coordination polymer network (Ce-PPi for short):
[0046] Use ultrapure water to prepare cerium nitrate hexahydrate solution and sodium pyrophosphate aqueous solution with a concentration of 50 mM, respectively. Quickly mix 2mL cerium nitrate hexahydrate solution and 2mL sodium pyrophosphate aqueous solution in a 10mL plastic centrifuge tube, vortex at 1500rpm for 5min, and the resulting white precipitate is Ce-PPi. The obtained suspension was centrifuged at 15,000 rpm for 10 min, the supernatant was discarded, the white precipitate was washed with ultrapure water, the above operation was repeated three times, and then freeze-dried to obtain Ce-PPi.
[0047] To the obtained Ce-PPi SEM (30kV), the results are shown in figure 1 . From figure 1 It can be concluded that the structure of the cerium ion-pyrophosphate coordination polymer network is a network structure composed of spherical nano-nodes, and the particle size of the nod...
Embodiment 2
[0053] The influence of glyphosate on the Ce-PPi fluorescence property of embodiment 1: 6 μ L glyphosate solution (500 μ M) and 20 μ L Ce 3+ The solution (0.5 mM) was mixed with 4 μL of PPi solution, and then the mixture was shaken at 600 rpm for 5 min at room temperature, and the fluorescence spectrum and the fluorescence intensity at 346 nm were recorded under excitation at 300 nm.
[0054] Fluorescence spectrum results see Figure 6 ,From Figure 6 It can be concluded that glyphosate significantly reduces the fluorescence intensity of Ce-PPi.
Embodiment 3
[0056] Ce-PPi fluorescence detection of glyphosate based on Example 1: 6 μL glyphosate solution and 20 μL Ce 3+ The solution (0.5 mM) was mixed with 4 μL of PPi solution, and then the mixture was shaken at 600 rpm for 5 minutes at room temperature, and the fluorescence spectrum and the fluorescence intensity at 346 nm were recorded under excitation at 300 nm.
[0057] Fluorescence spectrum results see Figure 7 ,From Figure 7 It can be drawn that the Ce-PPi fluorescence intensity changes with the glyphosate concentration.
[0058] Linear equation between fluorescence intensity and logarithm of glyphosate concentration (standard equation, Figure 8 is the standard curve): F=-4097.888logc+9356.104, R2=0.9972, F represents the fluorescence intensity, c represents the concentration of glyphosate, and the unit is μM / L.
[0059] The concentration linear range of glyphosate: 0.1-5μM, the detection limit is 2.37μg L-1 (0.014μM).
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