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Hemolytic agent for blood cell analysis

A technology of blood cell analysis and hemolytic agent, applied in the field of blood cell analysis, can solve the problems of difficult acquisition of raw materials, time-consuming detection and high cost, and achieve the effects of low cost, short detection time and easy acquisition.

Pending Publication Date: 2022-04-12
GENRUI BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The embodiment of the present invention provides a hemolytic agent for blood cell analysis, which can solve the technical problems of difficult acquisition of raw materials, high cost, time-consuming and labor-intensive detection

Method used

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  • Hemolytic agent for blood cell analysis
  • Hemolytic agent for blood cell analysis
  • Hemolytic agent for blood cell analysis

Examples

Experimental program
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preparation example Construction

[0033] The preparation process of the above-mentioned hemolytic agent for blood cell analysis is as follows: figure 1 Shown:

[0034] 100. First put the above components into a beaker and dissolve with a certain amount of pure water.

[0035] 101. Next, transfer the solution from the beaker to the volumetric flask with a glass rod.

[0036] 102. Then use a dropper to make up to 1 liter with pure water.

[0037] 103. After constant volume, shake it well.

[0038] In some embodiments, the cationic surfactants include octadecyltrimethylammonium chloride, dodecyltrimethylammonium chloride, dodecyltrimethylammonium chloride, cetyltrimethylammonium ammonium chloride, dodecyltrimethylammonium bromide, cetyltrimethylammonium bromide or combinations thereof.

[0039] In some embodiments, the concentration range of the octaalkyltrimethylammonium chloride is 0.50g / L-2.00g / L, and the concentration range of the dedecyltrimethylammonium chloride is 0.20g / L -1.50g / L, the concentration r...

Embodiment 1

[0061] Embodiment 1: A kind of hemolytic agent 1 for blood cell analysis:

[0062] In the hemolyzing agent for blood cell analysis provided in this embodiment, each liter of the hemolyzing agent includes cationic surfactant, nonionic surfactant, preservative, soluble salt, organic acid, and buffer. Among them, the cationic surfactant is 1.50g of octaalkyltrimethylammonium chloride and 0.15g of cetyltrimethylammonium chloride, and the nonionic surfactant is 0.30mL of 1,2-propanediol. The solvent is 1.50mL of phenoxyethanol, the soluble salt is 7.50g of sodium chloride and 2.00g of 1,3 dimethyl urea, the organic acid is 6.50mL of formic acid, and the buffer solution is 0.20mol of disodium hydrogen phosphate and 0.10 mol of citric acid. The pH of the hemolytic agent is 4.0.

Embodiment 2

[0063] Embodiment 2: A kind of hemolytic agent 2 for blood cell analysis:

[0064] In the hemolyzing agent for blood cell analysis provided in this embodiment, each liter of the hemolyzing agent includes cationic surfactant, nonionic surfactant, preservative, soluble salt, organic acid, and buffer. Wherein, the cationic surfactant is 0.25g of dodecyltrimethylammonium chloride and 0.15g of cetyltrimethylammonium chloride, and the nonionic surfactant is 0.30mL of 1,2-propanediol, The preservative is 1.50 mL of phenoxyethanol, the soluble salt is 7.50 g of sodium chloride and 0.15 g of sodium N-hydroxymethylglycinate, the organic acid is 6.50 mL of formic acid, and the buffer is 0.10 mol of citric acid. The pH of the hemolytic agent is 4.0.

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Abstract

The invention relates to the field of blood cell analysis, in particular to a hemolytic agent for blood cell analysis. The hemolytic agent for blood cell analysis is prepared from a cationic surface active agent, a nonionic surface active agent, a preservative, soluble salt, organic acid and a buffer solution, the pH value of the hemolytic agent ranges from 3.0 to 5.0; wherein the concentration range of the cationic surface active agent is 1.00 g / L to 10.00 g / L; the concentration range of the non-ionic surface active agent is 1.00 g / L to 5.00 g / L; the concentration range of the preservative is 0.50 mL / L to 2.00 mL / L; the concentration range of the soluble salt is 5.00 g / L to 20.00 g / L; the concentration range of the organic acid is 1.00 mL / L to 10.00 mL / L; the concentration range of the buffer solution is 0.50 g / L to 3.00 g / L. The hemolytic agent for blood cell analysis provided by the embodiment of the invention has the advantages of easily available raw materials, low cost and short detection time; meanwhile, under the action of the pH value, mononuclear cells, lymphocytes, neutrophils and eosinophils can be accurately and normally classified, so that white blood cells are kept in the original state, and high selectivity is achieved in the classification process.

Description

technical field [0001] The invention relates to the field of blood cell analysis, in particular to a hemolytic agent for blood cell analysis. [0002] technical background [0003] There are five types of white blood cells: monocytes, lymphocytes, neutrophils, eosinophils, and basophils. Among them, the increase of monocytes is common in chronic myelogenous leukemia, Hodgkin's disease and lead poisoning; the increase of lymphocytes is common in allergy, postoperative and burn; the increase of neutrophils is common in acute suppurative infection, After surgery, uremia, and acidosis; increased eosinophils are common in pertussis, tuberculosis, and hepatitis; increased basophils are common in typhoid, kala-azar, and monocytic leukemia. Therefore, in the field of medical clinical testing, the determination of the leukocyte classification in the patient's blood has important reference significance for the diagnosis and subsequent treatment of blood diseases. [0004] In the bloo...

Claims

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Application Information

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IPC IPC(8): G01N33/49G01N33/50
Inventor 廖耀彪余洋陈明
Owner GENRUI BIOTECH INC
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