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Penaeus vannamei breeding method based on detection of enterocytozoon hepatopenaei infection dose

A technology of shrimp hepatic Enterocystis and breeding method, which is applied in the field of Penaeus vannamei cultivation based on the detection of shrimp hepatic Enterococcus infection, can solve the problems of small use area, missed detection, and reduced operation, so as to reduce economic loss and cost Effect

Active Publication Date: 2022-04-15
INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU
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  • Abstract
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AI Technical Summary

Problems solved by technology

Among them, direct placement can reduce operations and reduce secondary environmental stress. Standard seedlings have the advantages of small use area, convenient centralized management, shortened breeding cycle, improved pond utilization rate, and reduced energy consumption costs. However, disease prevention and control is difficult. Seedlings need to undergo secondary environmental adaptation, and there are certain risks in improper operation
[0004] In the breeding process, seedlings, water quality, feed quality and management techniques are important factors that determine the success or failure of breeding, among which the quality of seedlings is the most critical, and the germplasm traits of seedlings Common pathogen carrying conditions have become the main screening items when farmers purchase seedlings. At present, farmers choose high-quality first-generation shrimp seedlings for breeding, and their growth and other traits have great advantages; seed pathogen quarantine has become an important method for seed selection. The seedlings that pass the quarantine can greatly reduce the risk of breeding, but the representativeness of seedling sampling samples can cause missed inspections, especially the pathogen of enterocystosis prawns is mostly carried by individuals. If the missed inspections are caused by insufficient sampling representativeness, It will lead to the horizontal transmission of pathogens during the breeding process, and the outbreak of enterocystosis hepatica, causing slow growth of prawns, seriously affecting the size and yield of adult shrimps
In addition, currently there is no effective control drug for Enteroplasma hepatica. How to deal with Enterosporidia hepatica is detected during the breeding process (especially in the middle and late stages of breeding), which has become a major problem for farmers.

Method used

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  • Penaeus vannamei breeding method based on detection of enterocytozoon hepatopenaei infection dose
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  • Penaeus vannamei breeding method based on detection of enterocytozoon hepatopenaei infection dose

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Embodiment 1

[0042] 1. Pond disinfection before breeding

[0043] 15-20 days before the breeding period, drain or add pond water level to 10-20cm, soak with 150-200kg quicklime (or 40-50kg bleaching powder and other disinfectants) per mu, during the soaking process, plow the bottom of the pond for 5-10cm , The pond stems are splashed with disinfectant water 2-3 times to ensure that the breeding ponds are thoroughly disinfected, and the disinfectant water is discharged after soaking for 3 days. If it is clear that there was Enterococcus hepatica infection in the previous batch of breeding, soak and disinfect again according to the previous method, and reduce the residual disinfectant solution by changing the water, etc., so as to meet the fishery water quality standards (GB 11607-89) such as pH and residual chlorine. The sediment at the bottom of the pool is tested for Enterocystoma hepatica according to the aquatic industry standard (SC / T7232-2020) to ensure that the breeding environment i...

Embodiment 2

[0066] A breeding experiment was carried out on a greenhouse farm of Penaeus vannamei in Yancheng, Jiangsu Province. There are 96 greenhouses in the base.

[0067] In 2019, according to the conventional breeding method, I chose to buy a certain brand of shrimp seedlings. Before purchasing the seedlings, no routine pathogen detection was carried out. The seedling size was about 1cm, and the stocking density was 80,000 fish / shed. The food intake and growth were obviously found after 40 days of breeding. The speed is slow. It was found that all the ponds of this batch of shrimp were infected with Enterococcus hepatica, and the quantitative data showed that the copies of Enterococcus hepatica in all pond shrimp samples exceeded 10000copies / ngDNA. The farmer continued to breed for 60 days. The size of the shrimp was 4-7cm, and the growth rate of the shrimp was about 30% slower than that of the same period last year. The copy volume of the shrimp was more than 50,000 copies / ngDNA. Du...

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Abstract

The invention discloses a penaeus vannamei breeding method based on detection of enterocytozoon hepatopenaei infection dose, and belongs to the technical field of penaeus vannamei breeding. According to the method, the infection dose of enterocytozoon hepatopenaei is regularly monitored in the whole culture period of the penaeus vannamei boone, and different culture measures are adopted for different infection doses. According to the method, high-quality shrimp seeds are selected for centralized thickening, pathogen indexes including enterocytozoon hepatopenaei are monitored regularly, and the shrimp seeds are cultivated in different pools after the shrimp seeds are subjected to thickening about one month and each item is detected to be qualified, so that compared with the technology of directly putting the shrimp seeds, on one hand, the cost is reduced through centralized management, and on the other hand, the risk of later-stage cultivation caused by missing detection of low disease-carrying rate of the shrimp seeds can be reduced. The vigor and ingestion growth conditions of the shrimps are observed at any time in the culture period, enterocytozoon hepatopenaei pathogens are monitored regularly, real-time fluorescent quantitative detection is supplemented if positive results are detected, treatment is carried out according to the method disclosed by the invention according to the culture period, the specification state of the shrimps and quantitative detection data, and the economic loss is reduced.

Description

technical field [0001] The application relates to the technical field of Penaeus vannamei culture, in particular to a culture method of Penaeus vannamei based on the detection of Enterococcus hepatica infection. Background technique [0002] Penaeus vannamei is the prawn species with the largest farming output in the world. Its farming is mainly distributed in 38 countries and regions including South America and Southeast Asia. The global annual output of Penaeus vannamei is about 5 million tons. my country's vannamei aquaculture and freshwater aquaculture are widely distributed, of which freshwater aquaculture is concentrated in 6 provinces and cities of Guangdong, Jiangsu, Fujian, Zhejiang, Shandong, and Tianjin, accounting for nearly 90% of the country's output; marine aquaculture is concentrated in Guangdong, Guangxi, Fujian, and Hainan , Shandong 5 provinces, the output accounts for more than 90% of the country. In 2020, the total output of Penaeus vannamei aquaculture ...

Claims

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Application Information

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IPC IPC(8): A01K61/59C12Q1/6893C12Q1/6851C12Q1/06
CPCY02A40/81
Inventor 沈辉乔毅蒋葛万夕和成婕范贤平曹晓慧黎慧王李宝史文军窦雅斌刘学实秦亚丽沈静孙瑞建
Owner INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU
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