Injectable temperature-sensitive hydrogel as well as preparation method and application thereof

A technology of temperature-sensitive hydrogel and preparation steps, which is applied in the field of biomedicine, can solve the problems of insufficient antibacterial concentration, limited curative effect of mechanical therapy, and increased bacterial resistance, so as to achieve the promotion of injectable performance, excellent antibacterial ability, and enhanced antibacterial The effect of the function

Pending Publication Date: 2022-04-15
AFFILIATED STOMATOLOGICAL HOSPITAL OF NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have not shown satisfactory efficacy in treatment due to the limited efficacy of mechanical therapy, increasing bacterial resistance, adverse drug reactions, and inability to achieve sufficient antibacterial concentrations.

Method used

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  • Injectable temperature-sensitive hydrogel as well as preparation method and application thereof
  • Injectable temperature-sensitive hydrogel as well as preparation method and application thereof
  • Injectable temperature-sensitive hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Weigh 1.4g of hydroxypropyl methylcellulose, 2g of hyaluronic acid, 0.1g of phentermine polypeptide and 2mL of glycerin;

[0020] (2) Add hydroxypropyl methylcellulose into 14mL of ultrapure water not lower than 80°C and stir well, stir well and then equilibrate overnight at 4°C to obtain a hydroxypropylmethylcellulose solution;

[0021] (3) Prepare hyaluronic acid with 20mL ultrapure water to make a solution with a mass concentration of 10%, and set it aside;

[0022] (4) Prepare the phenmu peptide with 1mL ultrapure water to make a solution with a mass concentration of 10%, and set it aside;

[0023] (5) Add 4 mL of the hyaluronic acid solution obtained in step (3) dropwise to the hydroxypropyl methylcellulose solution at room temperature, and stir evenly to obtain a mixed solution of hydroxypropyl methylcellulose / hyaluronic acid;

[0024] (6) Add 100 μL of the phentermine polypeptide solution obtained in step (4) dropwise to the hydroxypropyl methylcellulose / hya...

Embodiment 2

[0027] (1) Weigh 1.4 g of hydroxypropyl methylcellulose, 2 g of hyaluronic acid, 0.1 g of phentermine polypeptide and 3 mL of glycerin;

[0028] (2) Add hydroxypropyl methylcellulose to 13mL of ultrapure water not lower than 80°C and stir thoroughly, stir evenly and equilibrate overnight at 4°C to obtain a hydroxypropyl methylcellulose solution;

[0029] (3) Prepare hyaluronic acid with 20mL ultrapure water to make a solution with a mass concentration of 10%, and set it aside;

[0030] (4) Prepare the phenmu peptide with 1mL ultrapure water to make a solution with a mass concentration of 10%, and set it aside;

[0031] (5) Add 4 mL of the hyaluronic acid solution obtained in step (3) dropwise to the hydroxypropyl methylcellulose solution at room temperature, and stir evenly to obtain a mixed solution of hydroxypropyl methylcellulose / hyaluronic acid;

[0032] (6) Add 100 μL of the phentermine polypeptide solution obtained in step (4) dropwise to the hydroxypropyl methylcellulo...

Embodiment 3

[0035] (1) Weigh 1.4 g of hydroxypropyl methylcellulose, 2 g of hyaluronic acid, 0.1 g of phentermine polypeptide and 4 mL of glycerin;

[0036] (2) Add hydroxypropyl methylcellulose to 12mL of ultrapure water not lower than 80°C and stir well, stir evenly and equilibrate overnight at 4°C to obtain a hydroxypropyl methylcellulose solution;

[0037] (3) Prepare hyaluronic acid with 20mL ultrapure water to make a solution with a mass concentration of 10%, and set it aside;

[0038] (4) Prepare the phenmu peptide with 1mL ultrapure water to make a solution with a mass concentration of 10%, and set it aside;

[0039] (5) Add 4 mL of the hyaluronic acid solution obtained in step (3) dropwise to the hydroxypropyl methylcellulose solution at room temperature, and stir evenly to obtain a mixed solution of hydroxypropyl methylcellulose / hyaluronic acid;

[0040] (6) Add 100 μL of the phentermine polypeptide solution obtained in step (4) dropwise to the hydroxypropyl methylcellulose / hya...

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Abstract

The invention relates to injectable temperature-sensitive hydrogel as well as a preparation method and application thereof, hydroxypropyl methyl cellulose, hyaluronic acid and glycerol are taken as raw materials, Fenton polypeptide is carried, sol which can flow at room temperature is prepared, gel which can be converted into non-flow under physiological conditions is prepared, and the injectable temperature-sensitive hydrogel is medical injectable temperature-sensitive hydrogel containing Fenton polypeptide. The preparation method of the temperature-sensitive hydrogel comprises the following steps: mixing a hydroxypropyl methyl cellulose solution and a hyaluronic acid solution, uniformly stirring to obtain a solution A, adding a Fenton polypeptide solution into the solution A to obtain a solution B, finally dropwise adding glycerol into the solution B, and uniformly stirring to obtain the temperature-sensitive hydrogel system carrying Fenton polypeptide. The preparation method is simple, low in cost, free of cytotoxicity, good in antibacterial effect and convenient for subsequent popularization and application.

Description

technical field [0001] The invention belongs to the field of biomedical technology, and in particular relates to an injectable temperature-sensitive hydrogel for treating periodontal and peri-implant diseases and wounds, a preparation method and application thereof. Background technique [0002] Periodontitis and peri-implantitis are chronic diseases caused by plaque microorganisms attached to the surface of teeth and implants, leading to the destruction of periodontal and peri-implant tissues. Currently, the main treatment option is mechanical therapy supplemented with topical or systemic antibiotics or antiseptics. However, due to the limited efficacy of mechanical therapy, the increase of bacterial resistance, adverse drug reactions, and the inability to achieve sufficient antibacterial concentrations, these methods have not yet shown satisfactory efficacy in treatment. Therefore, it is of great scientific value to develop new therapeutic options to alleviate these pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K38/10A61K47/38A61K47/36A61K47/10A61P31/02A61P1/02A61P29/00A61L27/20A61L27/22A61L27/50A61L27/52A61L27/54
CPCA61K9/06A61K9/0019A61K38/10A61K47/38A61K47/36A61K47/10A61P31/02A61P1/02A61P29/00A61L27/20A61L27/227A61L27/50A61L27/52A61L27/54A61L2400/06A61L2300/252A61L2300/236A61L2300/404A61L2300/41A61L2300/412A61L2430/12C08L5/08C08L1/284C08L89/00
Inventor 汤春波吴迪吴维吴奇蓉赵桐吴瑾蔡坤展
Owner AFFILIATED STOMATOLOGICAL HOSPITAL OF NANJING MEDICAL UNIV
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