Uridine diphosphate preparation process based on multi-technology fusion

Abstract

The invention discloses a co-production preparation process for producing uridine diphosphate, adenosine triphosphate and uridine triphosphate by using beer yeast waste residues, which is characterized by comprising the following steps: firstly, collecting the beer yeast waste residues generated in a beer brewing process; carrying out enzymatic conversion by adopting a wall breaking agent; carrying out hydrolysis reaction on the yeast subjected to enzymatic conversion; deproteinizing and ultra-filtering the hydrolyzed mixed solution, desalting and concentrating the ultra-filtrate by using a desalting solution, and adsorbing nucleotide by using a column; carrying out graded elution on the nucleotide, and respectively collecting uridine diphosphate UDP, adenosine triphosphate ATP and uridine triphosphate UTP; adenosine triphosphate (ATP) and unconverted uridine triphosphate (UTP) are recovered; uDP is subjected to nanofiltration concentration, an obtained concentrated solution is subjected to decoloration adsorption, and UDP adsorption liquid is obtained; crystallizing and drying the uridine diphosphate UDP adsorption liquid to obtain uridine diphosphate UDP powder; and carrying out vacuum packaging on the uridine diphosphate UDP powder to complete the preparation of uridine diphosphate.

Description

technical field [0001] The invention belongs to the technical field of preparation of uridine diphosphate products, and specifically relates to a preparation process of uridine diphosphate based on the integration of multiple technologies. Background technique [0002] Beer waste yeast is rich in nutrients, contains nearly 50% protein, and contains various amino acids necessary for the human body. Cytochrome c, nucleic acid, glutathione (GSH), and reduced nicotinamide adenine can also be extracted from it. Dinucleotides (NADH), coenzyme A (CoA), etc., can only be used as feed additives at present, and how to develop higher added value is an imminent task. In recent years, my country's beer industry has developed rapidly, reaching 47.15 million tons in 2015. Brewer's yeast is the main by-product of beer production, accounting for about 1.5% of beer production, of which only about 40% of the yeast is recycled, and the remaining 60% is discarded. The use of beer waste yeast i...

AI Technical Summary

Problems solved by technology

The use of beer waste yeast in the production of nucleotide analogues such as adenosine triphosphate (ATP) is a high value-added utilization method, but there are also problems such as low yeast utilization rate, waste yeast enzyme solution that cannot be reprocessed, and waste of resources.

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