Anti-oxidation and anti-inflammatory egg white protein peptide
An egg white protein peptide and egg white technology, applied in the field of egg white protein peptide, can solve the problems of low preparation efficiency, single functional activity of egg white protein peptide, etc.
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Embodiment 1
[0028] A preparation of egg white protein peptide with anti-oxidation and anti-inflammation, comprising the following steps:
[0029] S1 pretreatment: Take 500g of egg white protein powder raw material, add water to prepare an egg white suspension with a concentration of 8wt%, then heat and denature it at 95°C for 30 minutes, and then homogenize the egg white suspension at 11000rpm for 2 minutes;
[0030] S2 enzymatic hydrolysis: take the pretreated homogeneous liquid in step S1 and adjust the pH value to 10.5 with 0.1mol / L sodium hydroxide aqueous solution, then heat to 50°C, add alkaline protease for enzymatic hydrolysis for 6 hours, add alkaline protease The amount is 2.6wt%. After the enzymolysis, inactivate the enzyme at 90°C for 15 minutes, and then cool to room temperature;
[0031] S3 Membrane Separation: Adjust the pH to 7.0 with 0.1mol / L hydrochloric acid aqueous solution of the solution after step S2 inactivated enzyme, then centrifuge at 6000r / min at 4°C for 15min,...
Embodiment 2
[0035] A preparation of egg white protein peptide with anti-oxidation and anti-inflammation, comprising the following steps:
[0036] S1 pretreatment: Take 500g of egg white protein powder raw material, add water to prepare an egg white suspension with a concentration of 8wt%, then heat and denature it at 95°C for 30 minutes, and then homogenize the egg white suspension at 11000rpm for 2 minutes;
[0037] S2 enzymatic hydrolysis: take the pretreated homogeneous liquid in step S1 and adjust the pH value to 10.5 with 0.1mol / L sodium hydroxide aqueous solution, then heat to 50°C, add alkaline protease for enzymatic hydrolysis for 6 hours, add alkaline protease The amount is 2.7wt%. After the enzymolysis, inactivate the enzyme at 90°C for 15 minutes, and then cool to room temperature;
[0038] S3 Membrane Separation: Adjust the pH to 7.0 with 0.1mol / L hydrochloric acid aqueous solution of the solution after step S2 inactivated enzyme, then centrifuge at 6000r / min at 4°C for 15min,...
Embodiment 3
[0042] A preparation of egg white protein peptide with anti-oxidation and anti-inflammation, comprising the following steps:
[0043] S1 pretreatment: Take 500g of egg white protein powder raw material, add water to prepare an egg white suspension with a concentration of 8wt%, then heat and denature it at 95°C for 30 minutes, and then homogenize the egg white suspension at 11000rpm for 2 minutes;
[0044]S2 enzymatic hydrolysis: take the pretreated homogeneous liquid in step S1 and adjust the pH value to 10.5 with 0.1mol / L sodium hydroxide aqueous solution, then heat to 50°C, add alkaline protease for enzymatic hydrolysis for 6 hours, add alkaline protease The amount is 2.8wt%. After the enzymolysis, inactivate the enzyme at 90°C for 15 minutes, and then cool to room temperature;
[0045] S3 Membrane Separation: Adjust the pH to 7.0 with 0.1mol / L hydrochloric acid aqueous solution of the solution after step S2 inactivated enzyme, then centrifuge at 6000r / min at 4°C for 15min, ...
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