Method for separating zeaxanthin and canthaxanthin in carotenoid through supercritical fluid chromatography

A technology of supercritical fluid and carotene, applied in separation methods, chemical instruments and methods, solid adsorbent liquid separation, etc., can solve problems such as inability to separate

Pending Publication Date: 2022-04-15
JIANGSU HANBON SCI & TECH CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the existing high-performance liquid chromatography detects that carotenoids contain a large amount of lutein, zeaxanthin and canthaxanthin, but their retention time in the liquid phase is almost the same, and there are other unknown carotenoid homologues Things mixed together cannot be separated

Method used

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  • Method for separating zeaxanthin and canthaxanthin in carotenoid through supercritical fluid chromatography
  • Method for separating zeaxanthin and canthaxanthin in carotenoid through supercritical fluid chromatography
  • Method for separating zeaxanthin and canthaxanthin in carotenoid through supercritical fluid chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] The chromatograph selected is a supercritical fluid chromatograph of Jiangsu Hanbang Technology Co., Ltd., with a chromatographic column size of 4.6×250 mm, a flow rate of the mobile phase system of 3 mL / min, a back pressure of 12 MPa, and a concentration of carotenoid methanol solution of 40 mg. / mL, the injection volume is 100μL, and the detection wavelength is 474nm.

[0094] Table 1 is the conditions of the method for separating zeaxanthin and canthaxanthin in carotenoids using supercritical fluid chromatography (SFC) in Example 1.

[0095] SFC separation condition in table 1 embodiment 1

[0096]

[0097] The specific steps corresponding to Example 1 are: now the carotenoid sample is dissolved in methanol, ultrasonically assisted, and configured into a 40 mg / mL carotenoid solution. On the supercritical fluid chromatograph of Jiangsu Hanbang Technology Co., Ltd., select Nucifera C18C 5μm 4.6×250mm is used as a chromatographic column, and the volume ratio, total ...

Embodiment 2

[0099] The chromatograph selected is a supercritical fluid chromatograph of Jiangsu Hanbang Technology Co., Ltd., with a chromatographic column size of 4.6×250 mm, a flow rate of the mobile phase system of 3 mL / min, a back pressure of 12 MPa, and a concentration of carotenoid methanol solution of 40 mg. / mL, the injection volume is 100μL, and the detection wavelength is 474nm.

[0100] Table 2 shows the conditions of the method for separating zeaxanthin and canthaxanthin from carotenoids using supercritical fluid chromatography (SFC) in Example 2.

[0101] SFC separation condition in table 2 embodiment 2

[0102]

[0103]

[0104] The specific steps corresponding to Example 2 are: now the carotenoid sample is dissolved in methanol, ultrasonically assisted, and configured into a carotenoid solution with a concentration of 40 mg / mL, and the carotenoid solution is placed on a supercritical fluid chromatograph of Jiangsu Hanbang Technology Co., Ltd. , select Nucifera phenyl...

Embodiment 3

[0106] The chromatograph selected is a supercritical fluid chromatograph of Jiangsu Hanbang Technology Co., Ltd., the column specification is 10×250mm, the flow rate of the mobile phase system is 10mL / min, the back pressure is set to 12MPa, and the concentration of the carotenoid methanol solution is 40mg / mL, the injection volume is 200μL, and the detection wavelength is 474nm.

[0107] Table 3 is the conditions of the method for separating zeaxanthin and canthaxanthin in carotenoids using supercritical fluid chromatography (SFC) in Example 3.

[0108] SFC separation condition in table 3 embodiment 3

[0109]

[0110]

[0111] The specific steps corresponding to Example 3 are: now the carotenoid sample is dissolved in methanol, ultrasonically assisted, and configured into a carotenoid solution with a concentration of 40 mg / mL, and the carotenoid sample is placed on a supercritical fluid chromatograph of Jiangsu Hanbang Technology Co., Ltd. , select two chromatographic ...

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Abstract

The invention belongs to the technical field of substance detection and separation, and provides a method for separating zeaxanthin and canthaxanthin in carotenoid through supercritical fluid chromatography. According to the method provided by the invention, supercritical carbon dioxide and lower alcohol are used as mobile phases, and a chromatographic column comprises a modified silica gel chromatographic column; the filler of the modified silica gel chromatographic column is silica gel of which the surface is bonded with amino, phenyl, cyano, diol group, C18P or C18C; the chromatographic column is a single chromatographic column or two chromatographic columns which are connected in series; separation and semi-preparation of zeaxanthin and canthaxanthin in carotenoid can be achieved, the separation time is short, and the separation effect is good.

Description

technical field [0001] The invention relates to the technical field of substance detection and separation, in particular to a method for separating zeaxanthin and canthaxanthin in carotenoids by supercritical fluid chromatography. Background technique [0002] Carotenoids are a general term for a class of important natural pigments, which are commonly found in yellow, orange-red or red pigments of animals, higher plants, fungi, and algae. It is an isopentenoid polymer containing 40 carbons, namely tetraterpenoids. A typical carotenoid is formed by connecting 8 isoprene units end to end. The color of carotenoids varies with the number of conjugated double bonds. The more the number of conjugated double bonds, the more the color shifts toward red. Carotenoids are the main source of vitamin A in the body, and also have anti-oxidation, immune regulation, anti-cancer, and anti-aging effects. At present, the existing high-performance liquid chromatography detects that caroteno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/34B01D15/42B01D15/18
Inventor 赵建强祁威李枝芳李浩
Owner JIANGSU HANBON SCI & TECH CO
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