Preparation method for eleutheroside

A technique for total glycosides of Acanthopanax senticosus and chromatography, which can be applied to chemical instruments and methods, medical preparations containing active ingredients, glycosides, etc., and can solve problems such as complex methods and the like.

Inactive Publication Date: 2013-01-23
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above method is relatively complex

Method used

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  • Preparation method for eleutheroside
  • Preparation method for eleutheroside
  • Preparation method for eleutheroside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1: Purify eleutherosides by gradient elution method, and optimize the concentration parameters of the eluent:

[0072] (1) Adopt existing technology to extract 28kg Acanthopanax, get 35L Acanthopanax extract, which is Acanthopanax aqueous extract, dissolve Acanthopanax aqueous extract with a small amount of methanol, ultrasonically make the dissolution even, use 0.45 μm microporous membrane filtration, the obtained filtrate is used for later use;

[0073] (2) Under normal temperature and pressure, use HPD-450 type macroporous adsorption resin as the stationary phase, fill the column with the stationary phase, and then use the macroporous resin column chromatography to separate the filtrate obtained in step (1), using 10% volume percentage in turn , 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% ethanol aqueous solution for elution, the amount of each concentration of ethanol aqueous solution is 3 to 4 times the column volume, collect each The eluent corresponding to th...

Embodiment 2

[0080] Embodiment two: Small scale scale-up experiment

[0081] Take 5kg of HPD-450 macroporous resin and pack it into a column. After pretreatment, wash with water until neutral. The column volume is 7L. Take 6.5L (184.6g alcohol extract) of Acanthopanax acanthopanax extract, and wash it with water, 20% by volume, and 30% ethanol aqueous solution in turn, and the volume of the eluent is 21L (3 column volumes). Concentrations of ethanol-water corresponding eluate fractions.

[0082] Then, as in Example 1, high performance liquid chromatography is used to analyze and detect the fractions corresponding to the eluents of each concentration. The difference is that the detection wavelengths are 203nm and 265nm; the results can be found in Figure 10-15 , it can be seen from the figure that 20% ethanol can not completely wash off the desired substance, and it is also demonstrated after repeated washing with 20% ethanol afterwards.

Embodiment 3

[0083] Embodiment 3: Small scale scale-up experiment (the eluting solution is ethanol aqueous solution with a volume fraction of 30%)

[0084] Take 5kg of HPD-450 macroporous resin and pack it into a column. After pretreatment, wash with water until neutral. The column volume is 7L. Take 6.5L (184.6g alcohol extract) of Acanthopanax acanthopanax extract, elute continuously with 30% by volume ethanol aqueous solution, collect the eluate fractions of each column volume respectively, and repeat 6 groups; then Each fraction is analyzed and detected, the detection method is with reference to Example 1, the detection wavelength is 203nm and 265nm, the results can be found in Figures 26-50 , it is known from the figure that the elution with 30% ethanol concentration is stable.

[0085] The 30% ethanol of the 2nd column volume in the embodiment is the high-performance liquid chromatogram of eluent corresponding fraction is analyzed, adopts absorbance method to quantitatively analyze...

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Abstract

The invention belongs to the field of traditional Chinese drug extracting, and relates to a method for extracting eleutheroside. The method comprises the following steps: (1) dissolving acanthopanax aqueous extract through methanol, then carrying out a ultrasonic process to uniformly dissolve the acanthopanax aqueous extract, followed by filtering through a micropore film to obtain filtrate to provide a spare use; (2) adopting a macroporous resin column chromatography for separating the filtrate prepared from the step (1), wherein a stationary phase is a HPD-450 macroporous adsorptive resin, a elution solvent 1 is a ethanol water solution having a volume percentage less than 30%, a elution solvent 2 is a ethanol water solution having a volume percentage more than 30%; sequentially adopting 3-6 times of column volume of the elution solvent 1 and the elution solvent 2 to carrying out elutions, and collecting eluent corresponding to the elution solvent 2. With the present invention, impurities are successfully separated from the eleutheroside, a content of the final extracted eleutheroside is high, and industrial cost for preparing the eleutheroside is low.

Description

technical field [0001] The invention belongs to the field of extracting traditional Chinese medicines, and relates to a method for extracting total glycosides of Acanthopanax senticosus. Background technique [0002] Acanthopanax is an Araliaceae plant, and its roots, rhizomes, stems, stems, leaves, and fruits can be used medicinally. Also known as Wujiashen and thorn crutches, it is mainly distributed in Northeast my country, North China, North Korea, Japan, Russia and other places. It has a long history of being widely used as a medicine in the motherland's medicine. It has the effects of replenishing qi, invigorating the spleen, invigorating the kidney and calming the nerves. It is used for spleen and kidney yang deficiency, physical weakness, loss of appetite, sore waist and knees, insomnia and dreaminess. [0003] One of the main components of Acanthopanax roots and rhizomes, the total glycosides are phenolic glycosides and flavonoid glycosides, which are one of its bi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07G3/00A61K36/254A61P1/14A61P25/20
Inventor 李贺然樊洁胡文李海霞
Owner SUZHOU UNIV
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