Unlock instant, AI-driven research and patent intelligence for your innovation.

T7 bacteriophage virus-like particle self-assembly method based on single plasmid

A phage and virus-like technology, applied in the field of self-assembly of T7 bacteriophage virus-like particles based on a single plasmid, can solve the problem of increasing capsid protein fusion antigens (difficulty in constructing vector vaccine antigens, poor genetic stability of multi-plasmids, insufficient single and definite components) And other issues

Active Publication Date: 2022-04-19
SICHUAN UNIV
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] (1) The components of the virus-like particles obtained by expressing the T7 phage gp10 gene and gp9 gene are not single and definite enough, which will increase the subsequent capsid protein fusion antigen ( The construction difficulty of carrier vaccine antigen)
[0012](2) The genetic stability of multi-plasmid is poor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • T7 bacteriophage virus-like particle self-assembly method based on single plasmid
  • T7 bacteriophage virus-like particle self-assembly method based on single plasmid
  • T7 bacteriophage virus-like particle self-assembly method based on single plasmid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0051] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0052] Aiming at the problems existing in the prior art, the present invention provides a method for self-assembly of T7 bacteriophage virus-like particles based on a single plasmid. The present invention will be described in detail below with reference to the accompanying drawings.

[0053] Such as figure 1 As shown, a single-plasmid-based T7 phage virus-like particle self-assembly method provided in the embodiment of the present invention includes the following steps:

[0054] S101, synthesize T7 phage gp9 and gp10A genes;

[0055] S102, construction of T7 phage virus-like particle pRSFDuet-1-gp9-gp10A expression plasm...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of self-assembly of virus-like particles (VLPs), and discloses a T7 bacteriophage virus-like particle self-assembly method based on a single plasmid, and the self-assembly method comprises the following steps: constructing an expression plasmid pRSFDuet-1-gp9-gp10A, transferring the expression plasmid into escherichia coli BL21 (DE3), and carrying out induction, expression, in-vivo self-assembly and preliminary purification to obtain the T7 bacteriophage virus-like particle; wherein the expression plasmid pRSFDuet-1-gp9-gp10A contains a capsid protein coding gene gp10A of the T7 bacteriophage and a scaffold protein coding gene gp9 of the T7 bacteriophage. The invention provides a new strategy for preparing the T7 bacteriophage virus-like particle, and lays a foundation for subsequent construction of a carrier vaccine or a targeted drug based on the T7 bacteriophage virus-like particle.

Description

technical field [0001] The invention belongs to the technical field of virus-like particles (Virus-like particles, VLPs) self-assembly technology, in particular to a method for self-assembly of T7 bacteriophage virus-like particles based on a single plasmid. Background technique [0002] T7 phage is a virulent phage whose host is Escherichia coli. Its original capsid (Procapsid) is a regular icosahedral lattice structure with T=7L conformation. It consists of the main capsid protein (Gp10), scaffold protein (Gp9), Connexin (Gp18) and core packing protein (Gp14 / Gp15 / Gp16). Among them, the capsid protein Gp10 has two forms: Gp10A and Gp10B. Gp10B is produced by a translational frameshift of the 341st amino acid of Gp10A, accounting for about 10% of the total capsid protein. [0003] Virus-like particles (VLPs) are protein particles assembled from one or more viral proteins and have a virus-like shape. They lack genetic material inside and cannot replicate, so they are not in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/70C12N15/64C12N15/34C07K14/01A61K39/385A61K47/46C12R1/92
CPCC12N15/70C07K14/005A61K39/385A61K47/46C12N2795/10222C12N2795/10252C12N2795/10223C12N2795/10234A61K2039/5258A61K2039/6075
Inventor 唐田杨加雪汪川朱娅岚方楚斌
Owner SICHUAN UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com