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Microbial soil improvement fungicide for preventing and treating tobacco epidemic diseases and preparation method of microbial soil improvement fungicide

A technology for soil improvement and tobacco blight, applied in the agricultural field, can solve problems such as difficulty in improving tobacco growth characteristics, inability to effectively improve tobacco heavy metals and related amino acid content, and achieve the effects of reducing damage, reducing cadmium accumulation, and reducing lysine

Active Publication Date: 2022-04-26
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0048] Aiming at the shortcomings and actual needs of the prior art, the object of the present invention is to provide a microbial soil improvement bacterial agent for preventing and treating tobacco blight and a preparation method thereof, so as to solve the following deficiencies in the prior art: (1) solve the problem of existing soil control methods Aiming at the common problem of low control efficiency of tobacco black shank and root black rot; (2) Solve the problem that it is difficult to improve the growth characteristics of tobacco under the condition of low control efficiency; (3) Solve the problem that existing soil control methods cannot Effectively improve the content of heavy metals and related amino acids in tobacco

Method used

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  • Microbial soil improvement fungicide for preventing and treating tobacco epidemic diseases and preparation method of microbial soil improvement fungicide
  • Microbial soil improvement fungicide for preventing and treating tobacco epidemic diseases and preparation method of microbial soil improvement fungicide
  • Microbial soil improvement fungicide for preventing and treating tobacco epidemic diseases and preparation method of microbial soil improvement fungicide

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Embodiment 1

[0083] 1. Raw material

[0084] Bacillus amyloliquefaciens cd5bc, the preservation number is CCTCC NO: M 2016232, commercially available;

[0085] Streptomyces oxysporum WS-29246, the preservation number is CCTCC NO: M 2012413, commercially available;

[0086] LB medium (for cultivating Bacillus amyloliquefaciens cd5bc): prepared by the laboratory;

[0087] Streptomyces oxysporum WS-29246 medium, prepared by the laboratory: sucrose 100g, glucose 10g, casamino acids 0.1g, yeast extract powder 5g, propanesulfonic acid 21g, K 2 SO 4 0.25g, MgCl 2 ·6H 2 O 10g, trace elements 1mL, water 1000mL, pH=7.0; the trace element formula is MnSO 4 ·7H 2 O 100mg, CuSO 4 ·7H 2 O 50mg, ZnSO 4 ·7H 2 O 100mg, CoCl 2 100mg, purified water 100mL;

[0088] The formula of the nutrient solution is: NH 4 NO 3 1.2g, KH 2 PO 4 1.5g, KCl 0.6g, ZnSO 4 ·7H 2 O 0.04g, Ca(NO 3 )·4H 2 O 3g, MgSO 4 ·7H 2 O 1g, FeSO 4 ·7H 2 O 1g, disodium edetate 0.6g, KI 0.2g, MnSO 4 0.1g, CuSO 4 0...

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Abstract

The invention provides a microbial soil improvement microbial agent for preventing and treating tobacco epidemic diseases. The microbial soil improvement microbial agent comprises bacillus amyloliquefaciens cd5bc rape straw fermentation products and streptomyces oxysporus WS-29246. The microbial soil improvement microbial agent comprises bacillus amyloliquefaciens cd5bc rape straw fermentation products and streptomyces oxysporus WS-29246, the preservation number of the bacillus amyloliquefaciens cd5bc is CCTCC (China Center For Type Culture Collection) NO: M 2016232; the preservation number of the streptomyces oxysporus WS-29246 is CCTCC (China Center for Type Culture Collection) NO: M 2012413; the bacillus amyloliquefaciens cd5bc rape straw fermentation product is prepared by taking rape straw as a fermentation substrate and inoculating bacillus amyloliquefaciens cd5bc as zymophyte for fermentation. The control effects of the composition on the tobacco black shank and the tobacco root black rot can reach 85% or above; cadmium accumulation in tobacco can be effectively reduced, lysine in the tobacco can be effectively reduced, the quality of the tobacco can be improved, harm to a human body is reduced, and the method has a good application prospect.

Description

technical field [0001] The invention belongs to the field of agricultural technology and relates to tobacco planting technology, in particular to a microbial soil improvement bacterial agent for preventing and treating tobacco blight and a preparation method thereof. Background technique [0002] In tobacco production, root black rot [1] and blackleg [2] It is a serious soil-borne disease, which has been highly concerned by researchers. Root black rot and black shank mainly occur in my country's Yunnan, Guizhou, Hubei, Hunan, and Sichuan, and the incidence rate can reach more than 30% in severely diseased fields. If not controlled, the damage will increase year by year. [0003] Root black rot is caused by the basal fungus Thielaviopsis basicola ), the hyphae are colorless at the beginning, then turn brown and have branches, and root black rot can occur in tobacco from the seedling stage to the adult plant stage, and root black rot has a huge impact on the yield and quali...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/14A01N63/28A01N63/22A01P21/00A01P3/00C09K101/00
CPCC09K17/14A01N63/28A01N63/22C09K2101/00
Inventor 敖金成李永梅李博
Owner YUNNAN AGRICULTURAL UNIVERSITY
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