Method for harvesting microalgae by utilizing fungus floating biological pad

A technology for floating organisms and microalgae, applied in microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve problems such as low efficiency and complex microalgae harvesting methods, to improve growth force and achieve continuity. Effects of harvesting, enhancing growth and harvesting efficiency

Pending Publication Date: 2022-04-26
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the above-mentioned technical problems, the purpose of the present invention is to provide a method for harvesting microalgae using fungal floating bio-mats to solve the problems that the existing microalgae harvesting methods are complex and inefficient

Method used

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  • Method for harvesting microalgae by utilizing fungus floating biological pad
  • Method for harvesting microalgae by utilizing fungus floating biological pad
  • Method for harvesting microalgae by utilizing fungus floating biological pad

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A method for harvesting microalgae using a fungal floating biological mat, comprising the following steps:

[0031] (1) At room temperature, Mucorus racemosa was cultured in YPD liquid medium for 8 days; wherein, the concentration of glucose in YPD liquid medium was 20g / L, the concentration of beef extract peptone was 10g / L, yeast extract The concentration is 5g / L;

[0032](2) In the microalgae-fungi culture device, add 3L BBM liquid culture medium (BBM liquid culture medium formulation sees Table 1), add 50mL Nishiki, then inoculate the Mucorus racemosa after step (1) cultivation into the microalgae In the algal-fungal incubator, the inoculation density is 2×10 6 OTU / mL (inoculation Mucor racemosus weight is 8.62g), cultivated at room temperature, on the 10th day, utilize Mucor racemosus planktonic mat to harvest Nishiki, the specific method of harvest Nishiki is: from feed port 7 The nutrient solution prepared in step (2) is added, and Nitzschia is automatically har...

Embodiment 2

[0034] A method for harvesting microalgae using a fungal floating biological mat, comprising the following steps:

[0035] (1) At room temperature, Mucorus racemosa was cultured in YPD liquid medium for 5 days; wherein, the concentration of glucose in YPD liquid medium was 22g / L, the concentration of beef extract peptone was 12g / L, yeast extract The concentration is 6g / L;

[0036] (2) In the microalgae-fungal incubator, add 3LBBM liquid medium (see Table 1 for the formula of BBM liquid medium), add 60mL diatoms, and then inoculate the microalgae with the mucormyces racemosa cultivated in step (2) -In the fungal incubator, the inoculation density is 1×10 6 OTU / mL, cultivated at room temperature, on the 10th day, utilize Mucor racemosus to harvest diatoms, and the specific method of harvesting diatoms is: add the nutrient solution prepared in step (3) from the feed port 7, At the same time, diatoms are automatically harvested from the discharge port 6 .

Embodiment 3

[0038] A method for harvesting microalgae using a fungal floating biological mat, comprising the following steps:

[0039] (1) At room temperature, Mucorus racemosa was cultured in YPD liquid medium for 10 days; wherein, the concentration of glucose in YPD liquid medium was 18g / L, the concentration of beef extract peptone was 8g / L, yeast extract The concentration is 4g / L;

[0040] (2) In the microalgae-fungi culture device, add 3L of BBM liquid medium (see Table 1 for the formula of BBM liquid medium), add 43mL of Scenedesmus, and then inoculate the microalgae with Mucor In the algal-fungal incubator, the inoculation density is 4×10 6 OTU / mL, cultivated at room temperature, on the 10th day, utilize the plankton mat of Mucor racemosus to harvest Scenedesmus, the specific method of harvesting Scenedesmus is: add the nutrient solution prepared in step (3) from feed inlet 7, At the same time, Scenedesmus is automatically harvested from the discharge port 6 .

[0041] Table 1 Co...

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Abstract

The invention discloses a method for harvesting microalgae by utilizing a fungus floating biological pad. According to the method, the microalgae are adsorbed by utilizing mucor racemosus, so that the purpose of harvesting the microalgae is achieved. The mucor racemosus can adsorb a large amount of microalgae biomass, and the mucor racemosus and the microalgae can float on the surface of a culture solution, so that the microalgae are harvested by utilizing the mucor racemosus floating biological pad, and the harvesting method is efficient and convenient. Besides, in the co-growth process of the mucor racemosus and the microalgae, the vitality of the microalgae can be enhanced, and the growth and reproduction of the microalgae can be promoted, so that the harvesting efficiency of the microalgae can be improved, and the growth of the microalgae can be enhanced.

Description

technical field [0001] The invention relates to the technical field of microalgae harvesting, in particular to a method for harvesting microalgae by using a fungal floating organism mat. Background technique [0002] Microalgae are a type of autotrophic microorganisms with high photosynthetic efficiency, which can produce a variety of metabolites. For example, some types of microalgae can accumulate a large amount of oil and fatty acids in their bodies during the growth and reproduction process. Microalgae can be applied in many fields, such as food, medicine, environmental governance and energy, therefore, microalgae is considered to be one of the most potential microorganisms. However, in the harvesting of microalgae, methods such as sedimentation, centrifugation, filtration, and flocculation are mainly used. Among them, sedimentation and filtration take a lot of time, and the harvesting efficiency is not high. Centrifugation requires a lot of power resources, and floccula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/14C12N1/38C12R1/89C12R1/785
CPCC12N1/12C12N1/14C12N1/38
Inventor 马文标李玫唐嘉陵李丽
Owner CHENGDU UNIV
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