Shell OTC marking method suitable for shellfish living body

A marking method, shellfish technology, applied in climate change adaptation, application, fish farming, etc., can solve the problems of shellfish growth interruption, etc., and achieve the effect of long-lasting marking

Pending Publication Date: 2022-04-29
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the high commercial value of the bivalves species, bivalves growth has been more intensively studied, testing shell growth rates using traditional methods (surface trimming, edge scoring, paint tags) may lead to interruptions in shellfish growth

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Taking the Philippine clam mark with a shell length of 4-5mm as an example, follow the steps below:

[0018] (1) Select 4-5mm Philippine clam seedlings, and clean the algae on the shell surface of the clams.

[0019] (2) Dissolve OTC in pure seawater, prepare OTC (250mg / L, 500mg / L, 750mg / L) dipped seawater with seawater, repeat three times, and set up a control group at the same time. With the increase of OTC concentration, the pH of seawater decreased gradually, and the pH of seawater was raised to 7-8 with sodium bicarbonate.

[0020] (3) Dip the philippine clam larvae in a 3L transparent small bucket at a density of 2000 grains / square meter for 72 hours, 30 grains per small bucket, during which they are normally fed twice a day and normally inflated to simulate a flowing seawater environment. After dipping gel imaging system (EpiChem 3 Darkroom) UV light detection whether the marking is successful. There is a strong light signal at the edge of the shell of clam p...

Embodiment 2

[0023] Taking the green clam mark with a shell length of 2mm as an example, follow the steps below:

[0024] 1) Select 1-2mm green clam seedlings, and clean the algae on the surface of green clams.

[0025] 2) Dissolve OTC in pure seawater, select the natural water body of shellfish natural living waters, use seawater to prepare OTC (750mg / L) soaked seawater, and set up a control group at the same time, three repetitions.

[0026] 3) Dye the clam larvae at 2000 grains per square meter in a 3L transparent small bucket for 72 hours, 30 grains per small bucket. During this period, they are normally fed twice a day and inflated normally to simulate the flowing seawater environment. Mirror (UV) to check whether the marking is successful. In the bright field state, there was no obvious change in the shell quality of the dipped green clam.

[0027] 4) The marked green clams were fed normally for 69 days, all the water was changed once every two days, and then the marks were checked...

Embodiment 3

[0029] Taking the red snail mark with a shell length of 1-2mm as an example, follow the steps below:

[0030] 1) Select the 1-2mm red snail seedlings, and clean the algae on the surface of the red snail.

[0031] 2) Dissolve OTC in pure seawater, select the natural water body of the natural living waters of shellfish, use seawater to configure three concentrations of OTC (750mg / L) infused seawater, and set up a control group at the same time, three repetitions.

[0032] 3) Dip the red snail larvae in a 3L transparent small bucket with 2000 grains / square meter for 72 hours, 30 grains in each small bucket. During this period, they are fed twice a day and inflated normally to simulate the flowing seawater environment. The sight glass (UV) checks whether the marking is successful. There was yellow fluorescent signal at the edge of the shell of the snail under the irradiation of ultraviolet light, and there was no obvious change in the stained snail in the bright field state.

[...

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PUM

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Abstract

The invention relates to a shell OTC marking method suitable for shellfish living bodies, and belongs to the technical field of aquatic animal marking, the method comprises the following steps: selecting shellfish fries with the shell length of 2-6mm, dissolving oxytetracycline hydrochloride in to-be-marked shellfish culture water, and continuously adjusting the pH value of seawater to 7-8 by using sodium bicarbonate or sodium hydroxide; the shellfish fries are dip-dyed in an oxytetracycline hydrochloride solution for 72 h, and during the period, microalgae are normally fed; the marked ruditapes philippinarum is normally fed, and light signals of the marked shellfish shells can be seen through a fluorophore sight glass. The shellfish fry cannot be touched or moved in the marking process, the physical effect on shellfishes is reduced to the minimum, marking is kept lasting on the premise that physiological damage is not caused to shellfish individuals, and pollution to the environment cannot be caused.

Description

technical field [0001] The invention relates to a shellfish marking method, in particular to a shellfish OTC marking method adapted to living shellfish. Background technique [0002] Aquatic animal marking technology can be used not only for marking and releasing aquatic animals and investigating their resources and activities, but also for large-scale selective breeding, such as family selection. In order to facilitate the distinction between different families and different treatment groups, it is necessary to mark the shellfish of different families and different treatment groups. Common methods of marking common shellfish include listing marks and molecular markings, and listing marks are easy to cause damage to shellfish. Molecular markers are not easy to cause harm to animals, but their detection is complicated and costly. The general requirements for the marking technology are that it does little harm to animals, is simple and easy to implement, low in cost and suit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/50A01K61/90
CPCA01K61/50A01K61/90Y02A40/81
Inventor 孙秀俊吴磊张天时涂康刘志鸿吴彪周丽青
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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