Shell OTC marking method suitable for shellfish living body
A marking method, shellfish technology, applied in climate change adaptation, application, fish farming, etc., can solve the problems of shellfish growth interruption, etc., and achieve the effect of long-lasting marking
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Embodiment 1
[0017] Taking the Philippine clam mark with a shell length of 4-5mm as an example, follow the steps below:
[0018] (1) Select 4-5mm Philippine clam seedlings, and clean the algae on the shell surface of the clams.
[0019] (2) Dissolve OTC in pure seawater, prepare OTC (250mg / L, 500mg / L, 750mg / L) dipped seawater with seawater, repeat three times, and set up a control group at the same time. With the increase of OTC concentration, the pH of seawater decreased gradually, and the pH of seawater was raised to 7-8 with sodium bicarbonate.
[0020] (3) Dip the philippine clam larvae in a 3L transparent small bucket at a density of 2000 grains / square meter for 72 hours, 30 grains per small bucket, during which they are normally fed twice a day and normally inflated to simulate a flowing seawater environment. After dipping gel imaging system (EpiChem 3 Darkroom) UV light detection whether the marking is successful. There is a strong light signal at the edge of the shell of clam p...
Embodiment 2
[0023] Taking the green clam mark with a shell length of 2mm as an example, follow the steps below:
[0024] 1) Select 1-2mm green clam seedlings, and clean the algae on the surface of green clams.
[0025] 2) Dissolve OTC in pure seawater, select the natural water body of shellfish natural living waters, use seawater to prepare OTC (750mg / L) soaked seawater, and set up a control group at the same time, three repetitions.
[0026] 3) Dye the clam larvae at 2000 grains per square meter in a 3L transparent small bucket for 72 hours, 30 grains per small bucket. During this period, they are normally fed twice a day and inflated normally to simulate the flowing seawater environment. Mirror (UV) to check whether the marking is successful. In the bright field state, there was no obvious change in the shell quality of the dipped green clam.
[0027] 4) The marked green clams were fed normally for 69 days, all the water was changed once every two days, and then the marks were checked...
Embodiment 3
[0029] Taking the red snail mark with a shell length of 1-2mm as an example, follow the steps below:
[0030] 1) Select the 1-2mm red snail seedlings, and clean the algae on the surface of the red snail.
[0031] 2) Dissolve OTC in pure seawater, select the natural water body of the natural living waters of shellfish, use seawater to configure three concentrations of OTC (750mg / L) infused seawater, and set up a control group at the same time, three repetitions.
[0032] 3) Dip the red snail larvae in a 3L transparent small bucket with 2000 grains / square meter for 72 hours, 30 grains in each small bucket. During this period, they are fed twice a day and inflated normally to simulate the flowing seawater environment. The sight glass (UV) checks whether the marking is successful. There was yellow fluorescent signal at the edge of the shell of the snail under the irradiation of ultraviolet light, and there was no obvious change in the stained snail in the bright field state.
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