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Method for detecting microbial phosphorus in wetland vegetation soil

A technology of soil microorganisms and wetland vegetation, which is applied in the field of soil microbial phosphorus detection in wetland vegetation, can solve problems such as the inability to predict the change trend of soil nutrients and the inability to give a dynamic evaluation strategy for soil nutrients, so as to reduce relative deviations, prevent environmental pollution, and promote Full effect of fumigation

Pending Publication Date: 2022-05-03
辽宁省德明环境检测有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the singleness of the soil microbial phosphorus detection scheme in the prior art, it is impossible to predict the microbial activity status and soil nutrient change trend, and cannot provide a dynamic evaluation strategy for soil nutrients; the present invention provides a wetland vegetation soil microbial phosphorus detection method, Wetland vegetation soil with active microbial reproduction is detected, and through interval culture detection, the trend of soil nutrients is predicted, and it provides reference value for formulating a reasonable plan to stabilize the soil environment

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  • Method for detecting microbial phosphorus in wetland vegetation soil
  • Method for detecting microbial phosphorus in wetland vegetation soil
  • Method for detecting microbial phosphorus in wetland vegetation soil

Examples

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Embodiment 1

[0058] A method for detecting microbial phosphorus in wetland vegetation soil, comprising the steps of:

[0059] Step 1, sampling, such as figure 1 Shown:

[0060] The water quality layer is sampled, and the middle part of the water quality layer is sampled with a straw to obtain water sample 1;

[0061] Sampling at the water-soil interface, using a straw or a sampler to take samples at the water-soil interface to obtain a mixed sample of water and soil 2;

[0062] Soil layer sampling: use a sampler to sample the soil layer at 5 cm below the soil layer to obtain shallow soil sample 3, and sample at 15 cm below the soil layer to obtain deep soil sample 4;

[0063] The water sample 1, the water-soil sample 2, the shallow soil sample 3 and the deep soil sample 4 are single-point sampling;

[0064] After the water sample 1, the mixed soil sample 2, the shallow soil sample 3 and the deep soil sample 4 are sampled, animal and plant impurities are cleaned;

[0065] Step 2, sub-sa...

Embodiment 2

[0105] A method for detecting microbial phosphorus in wetland vegetation soil, comprising the steps of:

[0106] Step 1, sampling, such as figure 1 Shown:

[0107] The water quality layer is sampled, and the middle part of the water quality layer is sampled with a straw to obtain water sample 1;

[0108] Sampling at the water-soil interface, using a straw or a sampler to take samples at the water-soil interface to obtain a mixed sample of water and soil 2;

[0109] Soil layer sampling: use a sampler to sample the soil layer 10cm below the soil layer to obtain shallow soil sample 3, and sample 30cm below the soil layer to obtain deep soil sample 4;

[0110] The water sample 1, the water-soil sample 2, the shallow soil sample 3 and the deep soil sample 4 are single-point sampling;

[0111] After the water sample 1, the mixed soil sample 2, the shallow soil sample 3 and the deep soil sample 4 are sampled, animal and plant impurities are cleaned;

[0112] Step 2, sub-sample:

...

Embodiment 3

[0152] A method for detecting microbial phosphorus in wetland vegetation soil, comprising the steps of:

[0153] Step 1, sampling, such as figure 1 Shown:

[0154] The water quality layer is sampled, and the middle part of the water quality layer is sampled with a straw to obtain water sample 1;

[0155] Sampling at the water-soil interface, using a straw or a sampler to take samples at the water-soil interface to obtain a mixed sample of water and soil 2;

[0156] Soil layer sampling: use a sampler to sample the soil layer 10cm below the soil layer to obtain shallow soil sample 3, and sample 20cm below the soil layer to obtain deep soil sample 4;

[0157] The water sample 1, the water-soil sample 2, the shallow soil sample 3 and the deep soil sample 4 are single-point sampling;

[0158] After the water sample 1, the mixed soil sample 2, the shallow soil sample 3 and the deep soil sample 4 are sampled, animal and plant impurities are cleaned;

[0159] Step 2, sub-sample: ...

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Abstract

The invention relates to a wetland vegetation soil microbial phosphorus detection method, which belongs to the technical field of microbial detection, and comprises the steps of sampling, sample separation, treatment, culture, fumigation, extraction, detection and the like. According to the microbial phosphorus detection method disclosed by the invention, wetland vegetation soil with active microbial reproduction is detected, the soil nutrient trend is prognosed through interval culture detection, and a reference value is provided for formulating a reasonable scheme to stabilize the soil environment. The method provided by the invention adopts silicon powder mixing, water content setting and coating to achieve the effect of complete fumigation and extraction, improves the detection accuracy, and can predict the generation speed of phosphorus element and the speed of converting phosphorus in soil although the specific types of microorganisms cannot be measured one by one, so that the advantage and disadvantage relationship of nutrients to microorganism breeding is judged; environmental pollution caused by excessive propagation of microorganisms is prevented.

Description

technical field [0001] The invention belongs to the technical field of microbial detection, and in particular relates to a method for detecting microbial phosphorus in wetland vegetation soil. Background technique [0002] In the soil environment, microbial biomass controls the cycling process of soil organic matter and major nutrients, and plays an important role in maintaining the nutrient supply of crops. In particular, the reproductive capacity of wetland soil microorganisms is related to the nutrient supply capacity of crops. Therefore, it is necessary to detect microbial phosphorus to judge the soil condition. [0003] In this regard, CN108548813A discloses a method for measuring soil microbial biomass phosphorus. The soil samples are divided into two groups, wherein, the first group of samples are fumigated with chloroform, and after removing chloroform, they are extracted by adding sodium bicarbonate for leaching. The first filtrate; the second group of samples wer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 刘明华王孟娟王志彪李苹辛志刚王晓杰毕建平
Owner 辽宁省德明环境检测有限公司
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