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Application of mangosteen extract alpha-mangostin in preparation of medicine for preventing and treating livestock and poultry clostridium perfringens infection

A technology for Clostridium perfringens and mangostin, which is applied in the directions of application, antibacterial drug, drug combination, etc., can solve the problems of neglect of biological activity and pharmacological effect, etc., and achieves low extraction cost, simple extraction process and high development. and research value effects

Pending Publication Date: 2022-05-10
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, untapped biological activities and pharmacological effects in traditional herbal medicines have been overlooked since the golden age of antibiotics

Method used

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  • Application of mangosteen extract alpha-mangostin in preparation of medicine for preventing and treating livestock and poultry clostridium perfringens infection
  • Application of mangosteen extract alpha-mangostin in preparation of medicine for preventing and treating livestock and poultry clostridium perfringens infection
  • Application of mangosteen extract alpha-mangostin in preparation of medicine for preventing and treating livestock and poultry clostridium perfringens infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Antibacterial activity assay of embodiment 1, AMG and derivatives thereof

[0041] According to the microbroth dilution method recommended by the American Clinical Laboratory Standardization Committee (CLSI 2021), AMG and its derivatives were tested for the minimum inhibitory concentration (MIC) of Clostridium perfringens in clinical chicken origin. The MIC results were judged mainly by reference to CLSI M100-31st Edition (2021) standard implementation.

[0042] The steps to detect the minimum inhibitory concentration are as follows:

[0043]1. Use FTG medium to dilute the drug to 10 concentration gradients, and add 100 μL / well to the first 10 wells of a 96-well U-shaped plate in sequence.

[0044] 2. Use FTG medium to dilute the strain to be tested (see Table 1) to obtain a strain concentration of 1×10 6 Add 100 μL / well of CFU / mL bacterial suspension to the wells where the drug has been added. Add 100 μL / well of FTG medium to the 11th row of wells as a negative cont...

Embodiment 2

[0069] The stability test of embodiment 2, AMG

[0070] 1. Light stability: Dissolve AMG in DMSO to prepare a solution with a concentration of 512 μg / mL. Wrap the compound in foil, place the compound at room temperature, and expose the compound to the sun for 3 hours. The antibacterial activity of S.aureus ATCC29213 and MRSA T144 was detected. The samples at room temperature were used as a control to investigate the antibacterial activity of AMG after different light treatments.

[0071] 2. Thermal stability: Dissolve AMG in DMSO to prepare a solution with a concentration of 512 μg / mL. After being divided into several portions on average, place them in water baths at -20°C, 4°C, 20°C-25°C (room temperature), 37°C, 40.5-42°C and 60°C for 1 hour. The antibacterial activity of S.aureus ATCC 29213 and MRSA T144 was detected. The samples at room temperature were used as a control to investigate the antibacterial activity of AMG treated at different temperatures.

[0072] 3. Ac...

Embodiment 3

[0074] Embodiment 3, evaluate the in vitro anti-inflammatory effect of AMG on gene and protein level

[0075] Cell complete medium: imported 1640 medium + 10% serum + 1% double antibody.

[0076] Preparation of LPS solution: Take 1mg of LPS powder, accurately weigh it, add 10mL of sterile PBS to prepare a 100μg / mL solution, aliquot, store at -20°C for later use, and dilute to 1ug / mL before use.

[0077] Based on the in vitro inflammatory model, the anti-inflammatory effect of plant-derived AMG was systematically evaluated by RT-qPCR and ELISA, using the main inflammatory factors of TNF-α, IL-6 and IL-1β as evaluation indicators.

[0078] 1. The specific steps of the experimental operation:

[0079] (1) First spread cell MH-S in a 12-well plate with a cell density of 1×10 6 cells / well, add 1 mL of culture medium to each well, and incubate overnight in a 37°C cell culture incubator.

[0080] (2) First add 1 mL of AMG with a concentration of 10 μmol, and use dexamethasone (DXM...

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Abstract

The invention provides application of mangosteen extract AMG and derivatives thereof in prevention and treatment of clinical pig, chicken and cattle-derived clostridium perfringens diseases (including type A, type B, type C, type D and type E). According to the present invention, the AMG has strong antibacterial activity on 79 A-type clostridium perfringens clinical isolation strains, can effectively treat chicken necrotic enteritis caused by C-type clostridium perfringens at a concentration of 4 mg / kg, and can significantly improve intestinal flora imbalance and repair damaged intestinal mucosa; in conclusion, AMG is wide in medicine source, low in extraction cost, simple in extraction process and easy to obtain, can effectively treat necrotizing enteritis caused by clostridium perfringens, and has high development and research value.

Description

technical field [0001] The present invention relates to the application of mangosteen extract, in particular to the extract α-mangostin (AMG) of Garcinia plant mangosteen exocarp, calyx, bark, stem, leaf and pseudocarp as veterinary drug additive in the prevention and treatment of clinical pigs and chickens. , and bovine-derived Clostridium perfringens (including: A-type, B-type, C-type, D-type and E-type) infection, belonging to the field of veterinary medicine. Background technique [0002] Antibacterial drugs play an important role in the treatment of human and animal diseases. However, the long-term use of antibiotics not only accelerates the generation and spread of drug-resistant pathogens, but also causes serious drug residues in animal products and the ecological environment, causing serious damage to human health. Big threats (Nature, 2016, 529, 336; Science, 2016, 352, 758). Under the background of "reducing and limiting resistance" in the global animal husbandry ...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P31/04A61P1/00A61K36/38A23K20/121A61K131/00A61K133/00A61K127/00A61K135/00
CPCA61K31/352A61P31/04A61P1/00A61K36/38A23K20/121
Inventor 朱奎沈建忠刘颖
Owner CHINA AGRI UNIV
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