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Preparation method and application of water-soluble organic hybrid polyoxometallate

A polyoxometalate and organic hybrid technology, applied in the field of medicine, can solve the problems of easy decomposition and poor water solubility of polyoxometalates, and achieve the effects of excellent anti-HPV activity and good cell penetration.

Active Publication Date: 2022-05-13
山西白求恩医院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the present problem of HPV virus suppression, the present invention proposes a method for preparing a hydrophilic organic-inorganic hybrid polyoxometalate that can be used for anti-HPV virus by carrying out hydrophilic organic modification on polyoxometalate
[0005] The present invention modifies the polyoxometalate based on the hydrophilic organic chain segment, which solves the problem of poor water solubility and easy decomposition of the polyoxometalate

Method used

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  • Preparation method and application of water-soluble organic hybrid polyoxometallate
  • Preparation method and application of water-soluble organic hybrid polyoxometallate
  • Preparation method and application of water-soluble organic hybrid polyoxometallate

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Dissolve 1 mmol of polyethylene glycol monooctyl ether with a molecular weight of 10,000 in 100 mL of tetrahydrofuran, add an equimolar amount of allyl bromide after deprotonation, react for 24 hours, and distill off the solvent under reduced pressure to obtain product A; Dissolve the vacant phosphotungstate in 100mL of acetonitrile, add 3mmol of mercaptotrimethoxysilane, reflux for 24 hours, cool to room temperature, and vacuum-dry to obtain product B; add 2mmol of product A and 1mmol of product B to 50mL of N , in N-dimethylformamide, add benzoin ether with a molar percentage of 2%, react at room temperature for 24 hours, centrifuge, and vacuum-dry the precipitate to obtain the water-soluble organic hybrid polyoxometalate against HPV virus Salt.

[0023] Cervical cancer SiHa cells were treated with the above-mentioned organic hybrid polyoxometalates at a concentration of 10 μmol / L. After 48 hours, the proliferation inhibition rate of cervical cancer SiHa cells was 92...

Embodiment 2

[0025] Dissolve 1 mmol of polyethylene glycol monostearyl ether with a molecular weight of 50,000 in 100 mL of tetrahydrofuran, add an equimolar amount of allyl bromide after deprotonation, react for 24 hours, and distill off the solvent under reduced pressure to obtain product A; Dissolve 1 mmol of vacant silicotungstate in 100 mL of acetonitrile, add 4 mmol of mercaptotrimethoxysilane, reflux for 24 hours, cool to room temperature, and dry in vacuum to obtain product B; add 2 mmol of product A and 1 mmol of product B to 200 mL Add azobisisobutyronitrile with a molar percentage of 10% to the N,N-dimethylformamide, react at room temperature for 24 hours, centrifuge, and vacuum-dry the precipitate to obtain the anti-HPV virus water-soluble organic Hybrid polyoxometalates.

[0026] Cervical cancer SiHa cells were treated with the above-mentioned organic hybrid polyoxometalates at a concentration of 10 μmol / L. After 48 hours, the proliferation inhibition rate of cervical cancer ...

Embodiment 3

[0028] Dissolve 1 mmol of polyethylene glycol monobutyl ether with a molecular weight of 30,000 in 100 mL of tetrahydrofuran, add an equimolar amount of allyl bromide after deprotonation, react for 24 hours, and distill off the solvent under reduced pressure to obtain product A; Dissolve the vacant phosphotungstate in 100mL of acetonitrile, add 2mmol of mercaptotriethoxysilane, reflux for 24 hours, cool to room temperature, and vacuum-dry to obtain product B; add 2mmol of product A and 1mmol of product B to 80mL of In N,N-dimethylformamide, add benzophenone with a molar percentage of 2%, react at room temperature for 24 hours, centrifuge, and vacuum-dry the precipitate to obtain the water-soluble organic hybrid compound against HPV virus. Oxymetalate.

[0029] Cervical cancer SiHa cells were treated with the above-mentioned organic hybrid polyoxometalates at a concentration of 10 μmol / L. After 48 hours, the proliferation inhibition rate of cervical cancer SiHa cells was 88.2%...

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Abstract

The invention discloses a preparation method and application of water-soluble organic hybrid polyoxometallate, belongs to the technical field of medicines, and can inhibit HPV (human papillomavirus) infection. The preparation method of the organic hybrid polyoxometallate comprises the following steps: modifying a terminal hydroxyl group of polyethylene glycol monoalkyl ether; carrying out sulfydryl silane modification on the vacancy polyoxometallate; and combining the sulfydryl modified polyoxometallate with the hydroxyl-terminated modified polyethylene glycol monoalkyl ether through a covalent bond reaction to obtain the organic hybrid polyoxometallate disclosed by the invention. The polyoxometallate is characterized in that the water solubility of the polyoxometallate is improved through the hydrophilic organic chain segment, meanwhile, the atomic cluster structure of the polyoxometallate is kept, and good anti-HPV activity is shown.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method and application of a water-soluble organic hybrid polyoxometalate. Background technique [0002] Human papillomavirus (HPV) is the causative agent of cervical cancer, the second most common cancer in women worldwide. Blocking the source of HPV has become an important strategy for the prevention and treatment of HPV-related tumors. Although HPV vaccines have been used to prevent and treat cervical diseases recently, there are still defects such as no preventive effect on non-vaccine HPV infection, no elimination of existing infection and related lesions, and no therapeutic effect on patients infected with HPV. It is of great significance to develop novel HPV virus inhibitors. [0003] Since the HPV virus is epitheliophilic, topical application to the infected site can inhibit the entry of HPV into the target cells and achieve the effect of block...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/24A61K33/42A61P31/20A61P35/00
CPCA61K33/24A61K33/42A61P31/20A61P35/00
Inventor 王赞宏张越王美琼王艺璇张韶华
Owner 山西白求恩医院
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