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Ampelopsis grossedentata extract as well as preparation method and application thereof in preparation of diuretic drugs

A vine tea extract and technology of vine tea, applied in application, drug combination, pharmaceutical formula, etc., can solve problems such as hindering application

Pending Publication Date: 2022-05-13
湖北省农业科学院中药材研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the diuretic and antihypertensive chemical active ingredients in vine tea have not been systematically extracted, which hinders their application in the development of more effective antihypertensive drugs and functional foods

Method used

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  • Ampelopsis grossedentata extract as well as preparation method and application thereof in preparation of diuretic drugs
  • Ampelopsis grossedentata extract as well as preparation method and application thereof in preparation of diuretic drugs
  • Ampelopsis grossedentata extract as well as preparation method and application thereof in preparation of diuretic drugs

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preparation example Construction

[0022] The invention provides a kind of preparation method of vine tea extract, comprises the following steps:

[0023] 1) mixing vine tea powder and water, extracting under ultrasonic to obtain the crude extract of vine tea;

[0024] 2) centrifuging the crude extract of vine tea through a filter bag to obtain a purified vine tea extract;

[0025] 3) Freeze-drying the purified vine tea extract to obtain a vine tea extract.

[0026] The invention mixes the vine tea powder and water, and extracts under ultrasonic to obtain the crude vine tea extract.

[0027] In the present invention, the particle size of the vine tea powder is an important factor affecting the extraction of active ingredients. If the particle size is too large, the effect of the solvent is insufficient; if the particle size is too small, it will cost costs and be harmful to the processing personnel. Experiments have proved that the particle size of the vine tea powder is preferably ≤75 μm, which is of great ...

Embodiment 1

[0048] (1) On May 23, 2020, 10kg of rattan tea raw materials were purchased from Tongcheba Rattan Tea Professional Cooperative in Xianfeng County, pulverized by a grinder (ZGP-2320Q), and passed through a 200-mesh sieve to obtain a rattan tea sample with a crushed particle size of ≤75 μm ;

[0049] (2) Using a kq5200e ultrasonic instrument, pure water is used as the extraction solvent, the ratio of material to liquid is 1:60 (the ratio of rattan tea to aqueous solution), the ultrasonic time is 40min, the temperature is 50°C, and the power is 190w, to obtain the extract;

[0050](3) The extract utilizes a centrifuge (model 200) to set 3000r / min, 1000 mesh filter bags for centrifugal filtration;

[0051] (4) Freeze drying machine (SCIENTZ-30F), freeze-drying conditions: vacuum degree 25MPa, temperature -40°C, time 12h, vine tea extract was freeze-dried to obtain vine tea extract with diuretic activity.

[0052] (5) According to the detection method provided by the patent "A Det...

Embodiment 2

[0054] (1) On June 11, 2020, 9.8 kg of rattan tea raw materials were purchased from Tongcheba Rattan Tea Professional Cooperative in Xianfeng County, pulverized by a pulverizer (ZGP-2320Q), and passed through a 400-mesh sieve to obtain rattan tea samples with a particle size of ≤ 38μm;

[0055] (2) Using the kq5200e ultrasonic instrument, pure water is used as the extraction solvent, the ratio of material to liquid is 1:60 (the ratio of rattan tea to aqueous solution), the ultrasonic time is 50min, the temperature is 55°C, and the power is 210w, to obtain the extract;

[0056] (3) The extract utilizes a centrifuge (model 200) to set 3000r / min, 1000 mesh filter bags for centrifugal filtration;

[0057] (4) Freeze drying machine (SCIENTZ-30F), freeze-drying conditions: vacuum degree 25MPa, temperature -45°C, time 12h, vine tea extract was freeze-dried to obtain vine tea extract with diuretic activity.

[0058] (5) According to the detection method provided by the patent "A Dete...

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Abstract

The invention provides an ampelopsis grossedentata extract, a preparation method thereof and application of the ampelopsis grossedentata extract in preparation of diuretic drugs, and belongs to the technical field of extraction of natural drug components. The ampelopsis grossedentata sample is extracted with water, ultrasonic assistance is carried out, and the obtained ampelopsis grossedentata extract comprises the main active ingredients of flavonoid substances such as dihydromyricetin larger than or equal to 280 mg / g, myricetin larger than or equal to 10 mg / g, myricetin larger than or equal to 30 mg / g, quercetin larger than or equal to 10 mg / g and taxifolin larger than or equal to 30 mg / g. An SD rat model is used for evaluating the diuretic activity and renal toxicity of the ampelopsis grossedentata extract, and the result shows that compared with a blank control group, the urine volume of SD rats of an ampelopsis grossedentata extract gavage treatment group is remarkably increased by more than or equal to 35% in the period of 2-6 h, and no renal toxicity exists. According to the method provided by the invention, the diuretic active ingredients in the ampelopsis grossedentata can be efficiently extracted, so that reference is provided for development and utilization.

Description

technical field [0001] The invention belongs to the technical field of extraction of natural medicinal components, and in particular relates to a rattan tea extract, a preparation method thereof and an application in the preparation of diuretics. Background technique [0002] Hypertension is the leading cause of death and disability in the world. Medical research shows that sodium intake is closely related to blood pressure. Diuretics can excrete sodium and diuretic, reduce blood volume, decrease cardiac blood output, and lower blood pressure. At present, the most commonly used antihypertensive drugs, diuretics, have side effects such as metabolic disorders and diuretic resistance. Therefore, it is urgent to develop new diuretic and antihypertensive drugs without side effects. Natural medicines derived from plants have the effect of lowering blood pressure through multiple channels, multiple systems, and multiple targets, and have less toxic and side effects than western me...

Claims

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Application Information

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IPC IPC(8): A61K36/87A61P7/10A61P9/12A23L33/105
CPCA61K36/87A61P7/10A61P9/12A23L33/105A61K2236/331A61K2236/53A61K2236/51A23V2002/00A23V2200/326
Inventor 李宇何美军冉亚兰罗建群谭旭辉赵仁君黄东海方昕悦刘玉
Owner 湖北省农业科学院中药材研究所