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Plasma preservation solution, blood collection tube and application of plasma preservation solution and blood collection tube in ctDNA detection

A technology for preserving liquid and plasma, which is used in specific-purpose bioreactors/fermenters, microbial determination/inspection, biomass post-processing, etc. , to achieve the effect of long storage time, avoiding ctDNA degradation and improving shelf life

Inactive Publication Date: 2022-05-13
南京兔牙医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is: when the plasma samples used for ctDNA detection are preserved, conventional storage reagents easily lead to the degradation of cfDNA, so that the samples cannot better meet the requirements of ctDNA sequencing

Method used

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  • Plasma preservation solution, blood collection tube and application of plasma preservation solution and blood collection tube in ctDNA detection
  • Plasma preservation solution, blood collection tube and application of plasma preservation solution and blood collection tube in ctDNA detection
  • Plasma preservation solution, blood collection tube and application of plasma preservation solution and blood collection tube in ctDNA detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] According to the following raw material concentrations, prepare the raw materials of each component and make a solution:

[0036] EDTA-K3 100mg / ml, diazosylurea 200mg / ml, glycine 40mg / ml, trehalose 20mg / ml, aurintricarboxylic acid 0.5mg / ml, dithiothreitol 15mg / ml.

Embodiment 2

[0038] The difference from Example 1 is that the concentration of dithiothreitol is changed to 5 mg / ml.

[0039] According to the following raw material concentrations, prepare the raw materials of each component and make a solution:

[0040] EDTA-K3 100mg / ml, diazosylurea 200mg / ml, glycine 40mg / ml, trehalose 20mg / ml, aurintricarboxylic acid 0.5mg / ml, dithiothreitol 5mg / ml.

Embodiment 3

[0042] The difference from Example 2 is that dithiothreitol is not added.

[0043] According to the following raw material concentrations, prepare the raw materials of each component and make a solution:

[0044] EDTA-K3 100mg / ml, diazolidinyl urea 200mg / ml, glycine 40mg / ml, trehalose 20mg / ml, aurintricarboxylic acid 0.5mg / ml.

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Abstract

The invention relates to a plasma preservation solution, a blood collection tube and application of the blood collection tube in ctDNA detection, and belongs to the technical field of liquid biopsy. Comprising 50 to 200 mg / ml of an anticoagulant, 50 to 300 mg / ml of a preservative, 10 to 100 mg / ml of an aldehyde quenching agent, 10 to 80 mg / ml of a cell membrane protective agent, 2 to 35 mg / ml of a metabolic inhibitor, 5 to 50 mg / ml of a free radical trapping agent and water. The plasma preserving fluid provided by the invention can effectively preserve a blood sampling sample, is especially suitable for a sample treatment process of ctDNA detection, and has the advantages of long storage time and avoidance of ctDNA degradation.

Description

technical field [0001] The invention relates to a plasma preservation solution, a blood collection tube and the application thereof in ctDNA detection, and belongs to the technical field of liquid biopsy. Background technique [0002] Liquid biopsy, as an emerging detection technology, is becoming more and more mature in the clinical application of tumors, and has become an important means for mutation gene detection, dynamic monitoring, and early cancer screening. Among them, blood-based liquid biopsy is the most important research direction, mainly for genetic detection of circulating tumor DNA (ctDNA), circulating tumor cells and exosomes in blood. ctDNA (circulating tumor DNA, circulating tumor DNA) refers to the DNA fragments from the tumor genome that carry certain characteristics (including mutation, rearrangement, copy number abnormality, methylation, etc.) that are constantly flowing in the human blood circulation system. The main sources of ctDNA include: from nec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6806C12M1/24
CPCC12Q1/6806A01N1/021A01N1/0226A61B5/15003A61B5/153C12Q2527/125
Inventor 赵瑾盛巧芸蒋亚辉韩良培张丽
Owner 南京兔牙医疗器械有限公司
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