Preparation method of sericin nanogel
A nanogel and sericin technology, applied in the field of nanomaterials, can solve problems such as short storage time, and achieve the effects of good stability, mild method conditions and simple process
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Embodiment 1
[0031] The preparation of sericin nanogel, concrete steps are as follows:
[0032] A extraction: cut 5g cocoon shell into 1cm 2 Size, add 200ml of ultra-pure water, heat at 120°C for 20min, centrifuge at 8000rpm for 5min to remove impurities, and treat with high temperature and high pressure (120°C, 20min) for later use;
[0033] B aging: use ultrapure water to prepare 5.4mM CaCl 2 Solution, after high temperature and high pressure (120 ℃, 20min) treatment, standby; Then the sericin solution that 6.25ml step A obtains and 25ml CaCl 2 Mix the solution and add ultrapure water to 50ml, age for 3d;
[0034] C Dialysis: dialyze the aged sericin solution with ultrapure water for 3 days, and change the water every 2 hours; the sericin nanogel can be obtained;
[0035] D freeze-drying: freeze-dry the obtained sericin nanogel solution at -20°C for 2 days to obtain sericin nanopowder;
[0036] E Preservation: mix the sericin nanogel obtained by dialysis with water (the mass concentr...
Embodiment 2
[0038] The preparation of sericin nanogel, concrete steps are as follows:
[0039] A extraction: cut 5g cocoon shell into 1cm 2 Size, add 200ml of ultra-pure water, heat at 120°C for 20min, centrifuge at 8000rpm for 5min to remove impurities, and treat with high temperature and high pressure (120°C, 20min) for later use;
[0040] B aging: use ultrapure water to prepare 5.4mM CaCl 2 solution and 3.2mM MgSO 4 Solution, high temperature and high pressure (120 ℃, 20min) after treatment for later use; Then the sericin solution that 6.25ml step A obtains, 12.5ml CaCl 2 solution and 12.5ml MgSO 4 Mix the solution evenly and add ultrapure water to 50ml, and age for 3 days; you can get sericin nanogel;
[0041] D freeze-drying: freeze-dry the aged sericin nanogel solution at -20°C for 2 days to obtain sericin nanopowder;
[0042] E Preservation: mix the sericin nanogel obtained by dialysis with water (the mass concentration of sericin nanogel is 0.05%), and store directly at 4°C; ...
Embodiment 3
[0052] The sericin nanogel obtained by dialysis in Example 1 was stored in a refrigerator at 4°C. After observation, no aggregation and precipitation occurred after storage for more than one month.
[0053] The sericin nanopowder obtained in Step D of Example 1 was stored in a -20°C freezer for 1 month, and then redissolved into an aqueous solution (the mass ratio of sericin nanopowder to water was 0.1:99.9). It was observed that sericin nanoparticles dispersed well in water and formed a transparent solution.
[0054] This proves that the sericin nanogel prepared by the method of the present invention has good stability, and the storage time in a liquid environment can reach at least 1 month; the freeze-dried powder can be stored for a long time.
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