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Extraction method of oil-tea cake protein and application of oil-tea cake protein

An extraction method and a technology for protein extraction liquid, which are applied in the preparation methods of peptides, protein adhesives, chemical instruments and methods, etc., can solve the problems of difficulty in controlling the extraction process and change the structural properties of proteins, and achieve low destructive, enhanced Dissolution rate, anti-oxidation effect

Pending Publication Date: 2022-05-27
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This document uses ultrasonic extraction, which will change the structural properties of the protein during ultrasonic treatment, and the extraction process is not easy to control

Method used

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  • Extraction method of oil-tea cake protein and application of oil-tea cake protein
  • Extraction method of oil-tea cake protein and application of oil-tea cake protein
  • Extraction method of oil-tea cake protein and application of oil-tea cake protein

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A method for extracting camellia cake protein, comprising the following steps:

[0029] (1) Pretreatment: add 6 times the amount of acetone to the camellia oleifera cake, stir quickly at 0°C to 2°C, filter, and rinse with trimethylsilyl ether to obtain a pretreatment material;

[0030] (2) Secondary pretreatment: add immobilized enzyme accounting for 10% of its mass and protein stabilizer accounting for 10% of its mass to the primary pretreatment material, and treat it at 30°C to 35°C for 5 hours to obtain secondary pretreatment. processing material;

[0031] (3) Differential pressure extraction: add the secondary pretreatment material to 8 times of sodium pyrophosphate buffer with a pH of 6, extract once at room temperature with a pressure difference of -0.1 MPa to 60 MPa, and filter to obtain Camellia oleifera Cake protein extract;

[0032] (4) Concentration and drying treatment: adjust the pH value of the Camellia oleifera cake protein extract to be 4.2, stand for ...

Embodiment 2

[0038] A method for extracting camellia cake protein, comprising the following steps:

[0039] (1) Pretreatment: add 8 times the amount of acetone to the Camellia oleifera cake, stir quickly at 0°C to 2°C, filter, and rinse with trimethylsilyl ether to obtain a pretreatment material;

[0040] (2) Secondary pretreatment: add immobilized enzyme accounting for 15% of its mass and protein stabilizer accounting for 10% of its mass to the primary pretreatment material, and treat it at 30°C to 35°C for 6 hours to obtain secondary pretreatment. material;

[0041] (3) Differential pressure extraction: add the secondary pretreatment material to 9 times of sodium pyrophosphate buffer with a pH of 6, extract once at room temperature with a pressure difference of -0.1 MPa to 60 MPa, and filter to obtain Camellia oleifera Cake protein extract;

[0042] (4) Concentration and drying treatment: adjust the pH value of the Camellia oleifera cake protein extract to be 4.2, stand for precipitati...

Embodiment 3

[0048] A method for extracting camellia cake protein, comprising the following steps:

[0049] (1) Pretreatment: Add 8 times the amount of acetone to the camellia oil cake, stir quickly at 2°C to 4°C, filter, and rinse with trimethylsilyl ether to obtain a pretreatment material;

[0050] (2) Secondary pretreatment: add immobilized enzyme accounting for 15% of its mass and protein stabilizer accounting for 15% of its mass to the primary pretreatment material, and treat it at 30°C to 35°C for 6 hours to obtain secondary pretreatment. material;

[0051] (3) Differential pressure extraction: add the secondary pretreatment material to 10 times of sodium pyrophosphate buffer with a pH of 6, extract once at room temperature with a pressure difference of -0.1 MPa to 60 MPa, and filter to obtain Camellia oleifera Cake protein extract;

[0052] (4) Concentration and drying treatment: adjust the pH value of the Camellia oleifera cake protein extract to be 4.2, stand for precipitation, ...

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Abstract

The invention belongs to the technical field of protein extraction, and particularly relates to an extraction method of oil-tea cake protein and application of the oil-tea cake protein. The extraction method comprises the following steps: (1) pretreatment: adding acetone into oil tea cakes, quickly and uniformly stirring at low temperature, filtering, and flushing with trimethylsilyl ether to obtain a primary pretreated material; (2) secondary pretreatment: adding immobilized enzyme and a protein stabilizer into the primary treated material, and performing enzymolysis treatment to obtain a secondary pretreated material; (3) differential pressure extraction: adding the secondary pretreated material into a buffer solution, performing differential pressure extraction, and filtering to obtain an extracting solution; and (4) concentrating and drying, namely adjusting the pH value of the extracting solution, standing to generate a precipitate, and freeze-drying the obtained precipitate to obtain the oil-tea cake protein. The extraction method of the oil-tea cake protein provided by the invention is high in oil-tea cake protein extraction rate, low in protein active group destructiveness and high in cross-linking reaction activity, and can be used for preparing a wood adhesive.

Description

technical field [0001] The invention belongs to the technical field of protein extraction, in particular to a method for extracting camellia cake protein and the application of the camellia cake protein. Background technique [0002] Camellia oleifera cake is the main by-product in the process of extracting camellia oil from Camellia oleifera fruit. Camellia cake is rich in protein, carbohydrates, saponins, etc. Correlation analysis shows that Camellia cake contains 19% to 25% crude protein, 35% to 40% sugar, 5% crude fat, 6% crude Fiber, 10% to 15% saponin. Camellia meal contains high crude protein, which is a good protein resource with development potential. [0003] At present, the extraction and application research of camellia cake protein is mainly concentrated in the food field. The extracted camellia cake protein has strong emulsification and oil absorption, and is used in ice cream, bakery products, meat products, imitation meat products and protein drinks. , The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/30C09J189/00
CPCC07K1/145C07K1/30C09J189/00Y02P60/87
Inventor 吴志刚梁坚坤余丽萍张本刚李利芬邓雪杨守禄
Owner GUIZHOU UNIV
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