Method for inhibiting growth of escherichia coli by using static magnetic field and method for balancing inhibiting effect of static magnetic field
A technology of Escherichia coli and static magnetic field, applied in the field of microbiology, can solve the problems of environmental sanitation and public health impact, the impact of microbial growth and its mechanism, etc. It is difficult to reach an agreement, and achieve the effect of easy observation and simple operation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0042] Example 1: Magnetic treatment method of Escherichia coli TOP10
[0043] In order to ensure the homogeneity of the initial growth state of Escherichia coli TOP10, the pre-treatment culture of TOP10 was homogenized, including:
[0044] like figure 1 As shown, Escherichia coli was first cultured in 5 mL LB liquid medium at 37°C and 200 rpm for 10 hours, and then was serially diluted with LB liquid medium to obtain 10 6 times the dilution of the bacterial solution. The dilution factor is also applicable to the colony statistical analysis process (including the number and diameter of colonies) in the following examples, while for the RNA-seq and metabolome analysis in the following examples, the dilution factor is ten times.
[0045] Take 10 μL of the diluted bacterial solution and inoculate it on the LB solid medium containing 1.5% agar, blow it dry in an ultra-clean table (no liquid flow), and then place it in an incubator for cultivation.
[0046] The culture dish of t...
Embodiment 2
[0048] Example 2: Effects of moderately strong static magnetic fields on bacterial growth
[0049]In this embodiment, the average steady-state static magnetic field strength of the E. coli growth plane is controlled by adjusting the distance from the magnet to the petri dish, thereby obtaining the dose-dependent effect of the static magnetic field strength on bacterial growth. Compared with Example 1, the only difference is that in this example, the average steady-state static magnetic field strengths of the E. coli growth plane are 210mT, 160mT, and 80mT, respectively, and the dilution factor is 10. 6 times.
[0050] The results showed that the diameter of CFUs after 210 mT static magnetic field treatment was still significantly smaller than that of the control group ( Figure 5 ), while the difference in diameter of CFUs disappeared after 160mT or 80mT static magnetic field treatment ( Figure 6-7 ). This shows that the biological effect of static magnetic field on the gr...
Embodiment 3
[0051] Example 3: Medium-strength static magnetic field treatment affects the carbon source utilization of Escherichia coli
[0052] In this example, transcriptome sequencing analysis (RNA-Seq) was performed on the colony samples obtained from the experimental group and the control group in Example 1 (4 independent replicate samples were taken from each group, and the dilution factor was ten times) to analyze the biological regulation of static magnetic field. learning process ( Figure 8 ). Among the detected genes, 202 genes were differentially expressed between the static magnetic field treatment group and the control group, of which 71.8% (145) of the differentially expressed genes (DEGs) were downregulated by the static magnetic field (indicated in black), 28.2% (57) The expression was up-regulated (indicated in light gray), and the maximum fold changes of up-regulated and down-regulated gene expression were 6.21 and 2.63, respectively.
[0053] Principal component anal...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More