Method for screening infertility related genes by using fruit fly model and application of method

Abstract

The invention relates to the technical field of fruit fly models, in particular to a method for screening infertility related genes by utilizing a fruit fly model and application of the method. The method disclosed by the invention comprises the following steps: S1, mating UAS-X-RNAi transgenic line fruit flies with tub-GAL4/CyO fruit flies, selecting F1-generation newly-emerged UAS-X-RNAi/tub-GAL4 male/female fruit flies as an experimental group, and selecting UAS-X-RNAi male/female fruit flies as a control group; s2, the F1 generation of newly emerged UAS-X-RNAi/tub-GAL4 male/female drosophila melanogaster and the UAS-X-RNAi male/female drosophila melanogaster obtained in the step S1 are mated with wild type female virgin drosophila melanogaster/male drosophila melanogaster; s3, observing and counting the number and hatching rate of offspring eggs and adults of the experimental group and the control group in the step S2; the X gene is a gene to be screened.

Description

technical field [0001] The invention relates to the technical field of fruit fly models, in particular to a method for screening infertility-related genes using a fruit fly model and an application thereof. Background technique [0002] Infertility was defined as having normal, unprotected sex for at least 12 months without a successful pregnancy. Infertility is a global health problem affecting approximately 12-15% of couples of reproductive age. Among infertile couples, female factors account for about 40-50%, male factors account for 25-40%, common factors account for 20-30%, and unknown causes account for about 10%, including tubal factors, ovulation disorders, and sperm production disorders. , Impaired sperm transport, abnormal sperm and other factors. All of these factors are regulated by genetic factors. [0003] Meiosis is a biological event unique to germ cells and the basis of biological sexual reproduction. During this process, pairing, synapsis and recombinat...

AI Technical Summary

Problems solved by technology

In recent years, with the development and popularization of high-throughput sequencing technology, multiple infertility-related genes have been discovered. The cytological description of the relevant molecular pathway mechanism occurred without functional and phenotypic verification

A few studies have used transgenic mice to study infertility. However, the use of transgenic mouse models has the disadvantages of time-consuming, expensive, and inability to perform large-scale screening.

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