Method for inducing calcium carbonate precipitation by combining plant urease with urease inhibitor

A urease inhibitor, calcium carbonate technology, applied in chemical instruments and methods, soil protection, fertilizer mixtures, etc., can solve the problems of large bacterial activity, difficult cultivation, instability, etc., to achieve slow reaction rate, good mechanical properties, The effect of improving uniformity

Pending Publication Date: 2022-06-07
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to overcome the above-mentioned shortcoming of the prior art, the object of the present invention is to provide a kind of method that plant urease combined with urease inhibitor induces calcium carbonate precipitation, to solve the complex and cumbersome process of bacterial culture in MICP technology in the prior art, the culture period is too long, bacteria Affected by the environment, the activity is unstable, and it is difficult to cultivate in large quantities; and the enzymatic reaction speed is difficult to control, so that the porosity of the rock and soil body decreases rapidly, causing the pores near the grouting holes to be blocked and the permeability of the grout to decrease continuously.

Method used

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  • Method for inducing calcium carbonate precipitation by combining plant urease with urease inhibitor

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Experimental program
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Effect test

Embodiment 1

[0041] 1. Extract plant urease. Soybeans were dried in an oven at 40°C for 6 hours. After crushing, the soybean flour was sieved with a 100-mesh steel sieve. The soybean flour was mixed with deionized water to prepare a soybean flour solution with a mass concentration of 50 g / L. Stir the soy flour solution evenly, filter it through gauze into a beaker, and after standing for 2 to 3 hours, take the supernatant of the beaker and place it in a centrifuge tube at 3000 r / min and 4°C for 15 minutes, and take the supernatant from the centrifuge tube. The solution was stored at 4°C.

[0042] 2. Make the cementitious liquid. Calcium chloride and urea were mixed with analytical reagent in a ratio of 1:1, the mass concentration of the solution was 0.25mol / L, and the urease inhibitor n-butyl thiophosphoric triamide or NBPT was added, accounting for 1% of the total solution volume.

[0043] 3. Grouting reinforcement. Using a peristaltic pump, the urease solution and the cementing soluti...

Embodiment 2

[0045]1. Extract plant urease. Soybeans were dried in an oven at 40°C for 6 hours. After crushing, the soybean flour was sieved with a 100-mesh steel sieve. The soybean flour was mixed with deionized water to prepare a soybean flour solution with a mass concentration of 60 g / L. Stir the soy flour solution evenly, filter it through gauze into a beaker, and after standing for 2 to 3 hours, take the supernatant of the beaker, put it in a centrifuge tube and centrifuge it at 3000 r / min and 4°C for 15 minutes. The solution was stored at 4°C.

[0046] 2. Make cementation solution. Calcium chloride and urea were mixed with analytical reagents in a ratio of 1:1, the mass concentration of the solution was 0.25 mol / L, and the urease inhibitor n-butyl thiophosphoric triamide (NBPT) was added, accounting for 1% of the total solution volume.

[0047] 3. Grouting reinforcement. Use a peristaltic pump to pour the urease solution and the cementitious solution into the sand in turn, and cur...

Embodiment 3

[0049] 1. Extract plant urease. The soybeans were dried in an oven at 40°C for 6 hours. After crushing, the soybean powder was sieved with a 100-mesh steel sieve. The soybean powder was mixed with deionized water to prepare a soybean powder solution with a mass concentration of 50 g / L. Stir the soy flour solution evenly, filter it through gauze into a beaker, and after standing for 2 to 3 hours, take the supernatant of the beaker, put it in a centrifuge tube and centrifuge it at 3000 r / min and 4°C for 15 minutes. The solution was stored at 4°C.

[0050] 2. Make cementation solution. Calcium chloride and urea were mixed with analytical pure reagent in a ratio of 1:1, the mass concentration of the solution was 0.7 mol / L, and the urease inhibitor n-butyl thiophosphoric triamide or NBPT was added, accounting for 0.1% of the total solution volume.

[0051] 3. Grouting reinforcement. Utilize peristaltic pump to pour urease solution and cementitious liquid into the pores of rock m...

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Abstract

The invention discloses a method for inducing calcium carbonate precipitation by using a urease inhibitor and plant urease, which belongs to the field of geotechnical engineering and rock mass reinforcement, and comprises the following steps: 1, extracting urease by using soybean; 2, adding a urease inhibitor into a mixed solution of calcium chloride and urea to prepare a cementing fluid; and 3, the urease solution and the cementing liquid are sequentially poured into the rock-soil body to be reinforced, and the rock-soil body can be reinforced after standing and maintenance. A long bacterial culture period is not needed, meanwhile, a small amount of urease inhibitor is added into the cementing liquid, the reaction rate is slowed down, blocking near a grouting opening is prevented, it can be guaranteed that grouting is conducted smoothly, sufficient infiltration of the cementing liquid is completed before pores are filled with calcium carbonate crystals, the rock mass reinforcement uniformity is improved, and the construction period is shortened. The method is suitable for reinforcing large-size complex fractured rock mass; good mechanical properties are achieved, and the expected reinforcing effect can be achieved.

Description

technical field [0001] The invention belongs to the fields of geotechnical engineering and rock mass reinforcement, in particular to a method for inducing calcium carbonate precipitation by combining plant urease with a urease inhibitor. Background technique [0002] MICP (Microbial Induced Carbonate Precipitation) is an emerging technology, which is mainly used to reinforce sand and rock mass in order to improve the mechanical properties of rock and soil mass. Compared with traditional cement slurry reinforcement, MICP technology has the characteristics of green environmental protection and environmental friendliness, and has become a hot research field. However, MICP technology also has shortcomings that cannot be ignored: the bacterial culture process is complicated and cumbersome, the culture period is too long, the activity of bacteria is greatly affected by the environment, and the activity is unstable, and it is difficult to cultivate in large quantities, which seriou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): E02D3/12C09K17/40
CPCE02D3/12C09K17/40Y02P60/21
Inventor 王军保程金开王晓鹏宋战平张玉伟
Owner XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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