Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for creating weak-photosensitivity japonica rice germplasm

A technology for japonica rice and germplasm, which is applied in chemical instruments and methods, botanical equipment and methods, biochemical equipment and methods, etc., to facilitate accurate selection, improve detection efficiency, and increase growth.

Active Publication Date: 2022-06-10
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]However, only creating a simple hd1+ehd1 weakly photosensitive japonica rice germplasm cannot meet the intricate stubble needs in different promotion areas, such as local farming systems, The safe full heading period and the best filling period have more specific requirements on the heading date of varieties, and more precise molecular regulation of the heading date (sowing full period) is required

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for creating weak-photosensitivity japonica rice germplasm
  • Method for creating weak-photosensitivity japonica rice germplasm
  • Method for creating weak-photosensitivity japonica rice germplasm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Screening and verification of genes related to heading date of parents Zhenjing 2400 and Jiahe 218

[0033] 1. Rice material

[0034] The rice line Zhenjing 2400 is derived from the rice breeding material MNU (methylnitrosourea) by mutagenesis. The complete name of the line is Zhenjing 2400 (Oryza sativa L. Japonica 2400), and its taxonomic status is Oryza genus of Poaceae has been preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: P201810, the preservation date is January 17, 2018, and the preservation address is Wuhan University, Wuhan, China, postcode 430072. Jiahe 218 is an early-maturing late japonica rice jointly bred by Jiaxing Academy of Agricultural Sciences and China Rice Research Institute.

[0035] 2. Primer design for Hd1, Ehd1 and SDG725 gene sequencing

[0036] The Hd1 allele in Zhenjing 2400 showed photoperiod insensitivity. Compared with the Hd1 gene in Jiahe 218, this allele had a 123bp insertion...

Embodiment 2

[0048] Example 2 Screening of homozygous families with different genotype combinations in Zhenjing 2400 / Jiahe 218 recombinant inbred lines

[0049] 1. Construction of population and phenotypic identification

[0050] (1) Using Zhenjing 2400 as the female parent and Jiahe 218 as the male parent, prepare a hybrid F 1 , followed by continuous selfing, using the single-seed method, a recombinant inbred line population was constructed, including 247 F 8 strain.

[0051] (2) The constructed recombinant inbred line populations were planted under the conditions of long-day sunshine (Jiangsu) and short-day sunshine (Hainan) respectively, and the full-earing date was investigated, and the full-sowing period (days from sowing to full-earing) was calculated. Phenotype identification, as follows:

[0052] The above-mentioned rice materials are planted twice a year. The first season is planted in the Xingxiang Experimental Base of Zhenjiang Agricultural Science Research Institute in the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for creating weak-photosensitivity japonica rice germplasm. The method comprises the following steps: (1) selecting a japonica rice line 1 with the genotype of hd1 + ehd1 + sdg725 and a japonica rice line 2 with the genotype of Hd1 + Ehd1 + SDG725 in the heading stage as parents; (2) performing continuous selfing after hybridization, and obtaining a recombinant selfing line group by using a single seed transmission method; and (3) screening homozygous families of different genotype combinations by using a molecular marker technology, and selecting families with small variation amplitude of full-heading period (days from sowing to full heading) and yield increasing potential under long-day and short-day conditions to obtain a new japonica rice strain with the genotype of hd1 + ehd1 + sdg725 (late-maturing line) or hd1 + ehd1 + SDG725 (early-maturing line) in the heading period. According to the low-sensitivity japonica rice strain cultivated by the method, the planting adaptability in southern rice areas can be improved, and the complex crop rotation requirements in different popularization areas can be selectively met. In addition, phenotypic selection of important agronomic traits in shuttle breeding and high-throughput genotyping of target genes Ehd1 and SDG725 are facilitated, and the breeding efficiency is improved.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and in particular relates to a method for creating germplasm of weakly light-sensitive japonica rice. Background technique [0002] The area of ​​japonica rice in China is about 150 million mu per year, almost 100% of which directly guarantees "food security"; it is mainly distributed in the Northeast rice region and the Jianghuai rice region. The planting areas of the three northeastern provinces and Jiangsu account for about 43.0% of the total area of ​​japonica rice in the country. and 26.0%. With the development of the economy and the improvement of people's living standards, the area of ​​japonica rice consumption has rapidly expanded from the original Northeast, Beijing, Tianjin, Shanghai, and Jiangsu and Zhejiang areas to large and medium-sized cities in Central South and South China. The south has become the largest japonica rice consumption area in China. In order to better mee...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04C12N15/29C12N15/54C12Q1/6895C12Q1/6858C12N15/11
CPCA01H1/02A01H1/04C07K14/415C12N9/1007C12Q1/6895C12Q1/6858C12Q2563/107C12Q2531/113
Inventor 孙立亭林添资龚红兵杨军景德道余波李闯钱华飞曾生元杜灿灿胡庆峰巫章平周义文费云燕
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products