Application of wood fiber prehydrolysis product in inhibition of generation of AGEs
A wood fiber, pre-hydrolysis technology, applied in the application, the function of food ingredients, plant/algae/fungus/moss components, etc., can solve problems such as utilization problems, and achieve broad application prospects and high-efficiency inhibitory effects.
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Embodiment 1
[0024] (1) Pulverize the wheat straw, air dry, take 50 g, mix with 500 mL of pure water, add it to the autoclave, stir continuously, and heat up to 190° C. and keep the temperature for 120 min.
[0025] (2) After the reaction is completed, the solid and liquid separation is realized by the filtration method, and the filtrate is collected, which is the pre-hydrolyzate.
[0026] (3) Glucose (Glu), bovine serum albumin (BSA) and sodium azide (bacteriostatic agent) were added to 0.01 mol / L PBS at pH 7.4, so that the final concentrations of the three were 0.2 mol / L, 10 mg / mL and 3 mmol / L.
[0027] (4) Take 5 mL of the solution of step (3), add 50 μL of the pre-hydrolysis solution of step (2), and heat at 50° C. for 2 days.
[0028] (5) The same volume of step (3) solution was taken and heated at 50°C for 2 days as a control group; the same volume of step (3) solution was taken without heating as a blank group.
[0029] (6) After taking out the reaction solution and cooling, use a...
Embodiment 2
[0034] (1) Pulverize the straw, air-dry, take 50 g, mix it with 500 mL of pure water, add it into the autoclave, stir continuously, and heat up to 190° C. and keep the temperature for 120 min.
[0035] (2) After the reaction is completed, the solid and liquid separation is realized by the filtration method, and the filtrate is collected, which is the pre-hydrolyzate.
[0036] (3) Glucose (Glu), bovine serum albumin (BSA) and sodium azide (bacteriostatic agent) were added to 0.01 mol / L PBS at pH 7.4, so that the final concentrations of the three were 0.2 mol / L, 10 mg / mL and 3 mmol / L.
[0037] (4) Take 5 mL of the solution of step (3), add 50 μL of the pre-hydrolysis solution of step (2), and heat at 50° C. for 2 days.
[0038] (5) The same volume of step (3) solution was taken and heated at 50°C for 2 days as a control group; the same volume of step (3) solution was taken without heating as a blank group.
[0039] (6) After taking out the reaction solution and cooling, use a ...
Embodiment 3
[0044] (1) Pulverize the wheat straw, air-dry, take 50 g, mix with 500 mL of pure water, add it to the autoclave, stir continuously, and heat up to 210° C. and keep the temperature for 120 min.
[0045] (2) After the reaction is completed, the solid and liquid separation is realized by the filtration method, and the filtrate is collected, which is the pre-hydrolyzate.
[0046] (3) Glucose (Glu), bovine serum albumin (BSA) and sodium azide (bacteriostatic agent) were added to 0.01 mol / L PBS at pH 7.4, so that the final concentrations of the three were 0.2 mol / L, 10 mg / mL and 3 mmol / L.
[0047] (4) Take 5 mL of the solution of step (3), add 50 μL of the pre-hydrolysis solution of step (2), and heat at 50° C. for 2 days.
[0048](5) The same volume of step (3) solution was taken and heated at 50°C for 2 days as a control group; the same volume of step (3) solution was taken without heating as a blank group.
[0049] (6) After taking out the reaction solution and cooling, use a ...
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