Novel ketamine rapid qualitative screening and accurate quantitative analysis method
A quantitative analysis, ketamine technology, applied in the analysis of materials, material excitation analysis, material analysis through optical means, etc., can solve problems such as difficult analysis and unsuitable for routine analysis
Pending Publication Date: 2022-06-28
福建警察学院
View PDF0 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Raman spectroscopy and IR techniques are simple, fast, highly sensitive, and non-destructive. However, Raman spectroscopy often has a strong fluorescent background. Substrate materials should be used to make surface-enhanced Raman spectroscopy. However, careful and invasive methods are still required. Sample preparation and therefore not suitable for routine analysis
IR technology is simple and fast for the analysis of seized solid synthetic drugs, but for liquid samples with complex matrices, it is difficult to analyze due to serious matrix background interference
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0018] The invention proposes a simple, efficient and sensitive indirect competitive fluorescence immunoquantitative KET detection method.
[0019] A novel method for rapid qualitative screening and accurate quantitative analysis of ketamine (KET), including the following steps:
[0020] In the first step, the ketamine hapten (KET-BSA) solution was slowly added dropwise to each microwell of the microplate, coated overnight, and then washed with PBST for use; in the second step, the KET-BSA was blocked with OVA solution In the third step, the ketamine monoclonal antibody (KET-Ab) is labeled with a fluorescent dye; the fluorescent dye-labeled KET-Ab is mixed with the sample solution to be tested or various KET standards The ketamine antigen (KET-Ag) in the solution was mixed to obtain a mixed solution, and the above mixed solution was added to different microwells of the microplate respectively; then KET-Ag / KET-Ab was incubated; Wash with PBST; in the fourth step, measure the f...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a novel ketamine rapid qualitative screening and accurate quantitative analysis method. KET in human blood, urine and environmental samples can be rapidly and quantitatively analyzed by adopting a competitive mode of coating a microwell plate with ketamine hapten. According to the competitive quantitative equation obtained by the method, the linear correlation coefficient is 0.992, the linear range is 0.01 mu g / mL-0. 5 mu g / mL, the detection limit is 0.1 pg / ml, and a specificity result shows that the cross reaction rate of interferents is lower than 10%. And the recovery rate of low-concentration, medium-concentration and high-concentration labeled samples is 96-117%. Compared with classic GC-MS / MS for detecting actual samples at the same time, the method has the advantage that the relative deviation of quantitative results is smaller than 10%. Moreover, compared with a GC-MS / MS method, the method disclosed by the invention has the advantages that the LOD value of the KET is four orders of magnitude lower, and the sample pretreatment and the economic investment have greater advantages compared with those of the classic GC-MS / MS method. And rapid, portable and sensitive qualitative and quantitative analysis of ketamine can be realized.
Description
technical field [0001] The invention belongs to the technical field of KET detection, in particular to a novel method for rapid qualitative screening and accurate quantitative analysis of ketamine. Background technique [0002] Due to the extensiveness, importance and urgency of the qualitative and quantitative analysis of ketamine (abbreviated as KET), researchers have used almost all known analytical methods in recent years, and new analytical methods are constantly emerging. Traditionally, chromatography, mass spectrometry (MS), and a combination of the two have been regarded as the gold standard for the analysis of addictive drugs, including: gas chromatography (GC) [1-4], liquid chromatography (LC) [4-6 ], GC-mass spectrometry (including single quadrupole gas chromatography-mass spectrometry GC-MS and tandem quadrupole gas chromatography-mass spectrometry (GC-MS / MS) [7, 8], LC-mass spectrometry [7, 8] 9–12], mass spectrometry [13, 14]. Although these techniques have ex...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6439
Inventor 曹洁陈小英
Owner 福建警察学院