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Method for accurately regulating and controlling alpha-glucan enzymolysis reaction and directionally extracting product with specific molecular weight and application of method

A technology of dextranase and dextran, which is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of no purified products, achieve mild conditions, reduce costs, and save production costs Effect

Pending Publication Date: 2022-07-01
GUANGDONG ACAD OF SCI INST OF BIOENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing technology, the aqueous phase extraction and purification technology is mostly applied to enzyme engineering or other high polymers are added to purify polysaccharides. There is no report on the direct participation of raw materials in the composition of the aqueous phase system to achieve the purpose of purifying products.

Method used

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  • Method for accurately regulating and controlling alpha-glucan enzymolysis reaction and directionally extracting product with specific molecular weight and application of method
  • Method for accurately regulating and controlling alpha-glucan enzymolysis reaction and directionally extracting product with specific molecular weight and application of method
  • Method for accurately regulating and controlling alpha-glucan enzymolysis reaction and directionally extracting product with specific molecular weight and application of method

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Example 1: Preparation of α-glucan with a weight-average molecular weight of about 200 kDa by precisely regulating the enzymatic hydrolysis reaction of α-glucan and directional extraction of specific molecular weight products

[0043] Reagents such as polyethylene glycol, potassium dihydrogen phosphate, ammonium sulfate, sodium sulfate, and magnesium sulfate were all of analytical grade.

[0044] Alpha-glucanase is obtained by fermentation and culture of Pichia pastoris engineering bacteria with deposit number CCTCC NO.2013096, and the preparation method of Pichia pastoris engineering bacteria with deposit number CCTCC NO.2013096 and α-glucanase It has been disclosed in patent 2013102877278. Alpha-glucan was prepared by single batch fermentation of shake flasks by Leuconostoc mesenteroides (Leuconostoc mesenteroides, purchased from Guangdong Provincial Microbial Culture Collection Center) with the deposit number of GDMCC1.473, and the specific fermentation process was 5...

Embodiment 2

[0054] Example 2: Method for preparing α-glucan with a weight-average molecular weight of about 70 kDa by precisely regulating the enzymatic hydrolysis reaction of α-glucan and directional extraction of specific molecular weight products

[0055] Alpha-glucanase is obtained by fermentation and culture of Pichia pastoris engineering bacteria with deposit number CCTCC NO.2013096, and the preparation method of Pichia pastoris engineering bacteria with deposit number CCTCC NO.2013096 and α-glucanase It has been disclosed in patent 2013102877278. The α-glucan was prepared from Leuconostoc mesenteroides with the deposit number GDMCC1.473 (purchased from the Guangdong Provincial Microbial Culture Collection Center) by single batch fermentation in shake flasks. The specific fermentation process is 2.7L of 5L fermentation tank, the inoculum volume is 10% (v / v), the initial pH is 6.5, the culture temperature is 25°C, and the fermentation is carried out under the conditions of 100r / min. ...

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Abstract

The invention discloses a method for accurately regulating and controlling alpha-glucan enzymolysis reaction and directionally extracting a specific molecular weight product and application of the method, and belongs to the technical field of biochemical separation. Glucan is not only a polymer capable of forming a phase body, but also the molecular weight of glucan is a key factor influencing a phase forming condition, and the final target molecular weight of the enzymolysis reaction falls on a phase splitting point by adjusting the phase body composition of other high polymers. And meanwhile, the high partition coefficient of the alpha-glucanase in part of the polymer phase is utilized to quickly recover enzyme protein from the reaction phase and stop the enzymolysis reaction, and after the low-molecular-weight alpha-glucan is separated, the enzyme is recovered again through a specific concentrated salt solution which is compatible with the alpha-glucanase protein.

Description

technical field [0001] The invention belongs to the technical field of biochemical separation, and relates to an extended application of an aqueous phase extraction technology, in particular to a method for accurately regulating an α-glucan enzymatic hydrolysis reaction and directional extraction of a specific molecular weight product and its application. Background technique [0002] The production process of low molecular weight α-glucan currently required by the food or pharmaceutical industry is: fermentation-ethanol precipitation-hydrolysis-separation and purification-lyophilization. Among them, the hydrolysis methods mainly include enzymatic hydrolysis and acid hydrolysis. There are also methods to carry out the fermentation and hydrolysis processes at the same time. For example, in CN101205257, high molecular weight α-glucan is obtained by fermentation and then acid hydrolyzed to the target molecular weight product, and then passed through a multi-stage membrane. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/08C07H1/06C07H3/02
CPCC12P19/08C07H1/06C07H3/02
Inventor 黎志德张九花黄曾慰梁达奉柳颖刘桂云常国炜
Owner GUANGDONG ACAD OF SCI INST OF BIOENGINEERING
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