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Method for detecting resistance of litchi to peronophythora litchii and application of method

A technology of litchi downy mildew and litchi downy Phytophthora, which is applied in the field of plant disease resistance, can solve the problems of inconsistent fruit maturity period, high intensity of disease resistance screening, and inability to carry out disease resistance detection, achieving low cost and short time consumption Effect

Pending Publication Date: 2022-07-01
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Conventional detection methods are mainly aimed at the ripe fruit of litchi, however, litchi ripens once a year, and the fruit maturity period is inconsistent, and it is intensive to screen a large number of fruits for disease resistance in a relatively short period of time; Litchi materials, also can not be tested for disease resistance

Method used

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  • Method for detecting resistance of litchi to peronophythora litchii and application of method
  • Method for detecting resistance of litchi to peronophythora litchii and application of method
  • Method for detecting resistance of litchi to peronophythora litchii and application of method

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Experimental program
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Effect test

Embodiment 1

[0045] Embodiment 1 A kind of method of detecting lychee resistance to lychee downy mildew

[0046] A method for detecting lychee resistance to lychee downy mildew of the present embodiment comprises the following steps:

[0047] (1) Pick 30 fresh lychee leaves of cinnamon flavor (about 10 days after germination, soft and gradually turn green), spread them flat in a petri dish, put 3 fresh lychee leaves in each petri dish, and add 10 mL of sterile water ;

[0048] (2), dilute the spore suspension of Phytophthora lychee to the spore number in the spore suspension is 4*10 4 pcs / mL;

[0049] (3), in the fresh leaf of litchi of step (1), inoculate the Phytophthora spore suspension of 5uL step (2) with pipette;

[0050] (4) Cultivate the inoculated fresh lychee leaves in a constant temperature incubator at 27°C for 12h / 12h day and night.

[0051] (5), the 4th day of inoculation, observe that the spot area of ​​litchi fresh leaves is grade 9, and calculate the disease index (DI) t...

Embodiment 2

[0052] Embodiment 2 A kind of method for detecting lychee resistance to lychee downy mildew

[0053] A method for detecting lychee resistance to lychee downy mildew of the present embodiment comprises the following steps:

[0054] (1), pick 30 pieces of citrus lychee fresh leaves (10 days of germination, soft and gradually turn green), spread in a petri dish, put 3 pieces of lychee fresh leaves in each petri dish, add 10mL of sterile water;

[0055] (2), dilute the spore suspension of Phytophthora lychee to the spore number in the spore suspension is 3*10 4 pcs / mL;

[0056] (3), in the fresh leaf of litchi of step (1), inoculate the Phytophthora lychee spore suspension of 4uL step (2) with pipette gun;

[0057] (4) Cultivate the inoculated fresh litchi leaves in a constant temperature incubator at 25°C for 12h / 12h day and night.

[0058] (5), on the 4th day of inoculation, observe and count the lesion area of ​​fresh leaves of litchi to be grade 3-5, and calculate the disea...

Embodiment 3

[0059] Embodiment 3 A kind of method of detecting lychee resistance to lychee downy mildew

[0060] A method for detecting lychee resistance to lychee downy mildew of the present embodiment comprises the following steps:

[0061] (1), pick Feizixiao lychee fresh leaves (about 12 days of germination, soft and gradually turn green), spread in a petri dish, put 3 pieces of lychee fresh leaves in each petri dish, and add 10mL of sterile water;

[0062] (2), dilute the spore suspension of Phytophthora lychee to the number of spores in the spore suspension is 5*10 4 pcs / mL;

[0063] (3), in the lychee fresh leaf of step (1), inoculate the Phytophthora downy mildew suspension of 8ul step (2) with pipette;

[0064] (4) Cultivate the inoculated lychees in a constant temperature incubator at 28°C for 12h / 12h day and night.

[0065] (5), on the 4th day of inoculation, the diseased spot area of ​​observation and statistics litchi is grade 9, and the disease index (DI) of calculating Ph...

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Abstract

The invention discloses a method for detecting litchi peronophythora blight resistance of litchis and application of the method, and the method comprises the following steps: inoculating peronophythora blight spore suspension on each fresh leaf of the litchis; culturing the inoculated fresh litchi leaves at a constant temperature for 2-4 days; and detecting whether the litchi resists the peronophythora litchii or not according to the disease index of the fresh litchi leaves infected by the peronophythora litchii. The detection method disclosed by the invention is accurate and reliable, can be used for carrying out peronophythora litchii resistance phenotype detection on litchi materials in a large scale in multiple periods in one year, is short in time consumption and low in cost, and can be widely applied to disease-resistant breeding of litchis.

Description

technical field [0001] The invention belongs to the technical field of plant disease resistance, and more particularly, the invention relates to a method for rapid detection of lychee resistance to Phytophthora lychee downy mildew and its application. Background technique [0002] Disease is an important limiting factor affecting the growth and development of plants. In agricultural production, serious diseases can cause a significant reduction in crop yield or quality. Therefore, research on plant disease resistance is of great significance to agricultural production. [0003] Phytophthora lychee downy mildew is the most serious disease on lychees caused by the infection of Phytophthora lychee downy mildew. Fruit, sometimes also damage young fruit, flower spikes and leaves. [0004] It is very important to screen excellent materials with high resistance to Phytophthora downy mildew. Conventional detection methods are mainly aimed at the mature fruit of lychee. However, l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12Q1/24C12R1/645
CPCC12Q1/18C12Q1/24G01N2333/37
Inventor 刘海伦严倩欧良喜
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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