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System and method for optically detecting food-borne pathogenic bacteria

A technology for optical detection of food-borne pathogens, applied in the measurement of color/spectral characteristics, etc., can solve the problems of cell-level detection of food-borne pathogens, slow imaging speed, and cell apoptosis.

Pending Publication Date: 2022-07-01
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, patent CN201310309189.8 uses Raman spectroscopy technology to assist microscope imaging. However, the imaging speed of traditional Raman scattering technology is slow, and the new enhanced Raman technology based on nanomaterials still faces problems such as cell apoptosis, which is not easy to cause food-borne pathogens. Detection of pathogens at the cell level

Method used

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  • System and method for optically detecting food-borne pathogenic bacteria
  • System and method for optically detecting food-borne pathogenic bacteria
  • System and method for optically detecting food-borne pathogenic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] (1) Turn on the power of each component of the system 100, and the system 100 is initialized;

[0066] (2) The light path of the microscope is set to the dark field mode, and the light source system 100 is placed away from the main body of the microscope to avoid heat burning the cells;

[0067] (3) Use a pipette to suck out 3 μL of the mixed sample of bacterial liquid and place it in the center of the glass slide 16, use the biological cabinet to dry the sample preparation, drop 2 drops of sterile water before the experiment, and use the cover glass to squeeze out excess air bubbles. Drop pine oil on top of the coverslip and wait for observation with the 100X oil lens in the microscope.

[0068] (4) Adjust the angle of the bottom illumination fiber and the reflective prism to ensure that the light beam can enter the shutter 50 normally.

[0069] (5) Adjust the spatial position of the stage 14 so that the glass slide 16 can be in close contact with the 100X oil lens, a...

Embodiment 2

[0077] Adopt the same step (1) to step (10) with embodiment 1, in addition, also comprise the following steps:

[0078] (11) Repeat the above steps to collect images and data for more pathogenic bacteria samples until completion.

[0079] (12) Adjust the 100X oil lens to a 20X ordinary objective lens, take out the glass slide 16, lower the spatial position of the stage 14, turn off the illumination system 100 of the hyperspectral microscope 100, and wipe the 100X oil lens with a non-woven fabric.

[0080] (13) Turn off the hyperspectral imager 32 and the enhanced enhanced charge-coupled camera 31 in sequence, and after 10 minutes, clean the microscope as a whole, and cover it with a dust-proof cloth.

[0081] (14) Export the data using a mobile hard disk, and organize the data to form a spectral data set of the tested sample.

[0082] (15) Put the spectral data set into the established spectroscopic analysis model, through the automatic search of different types of cell spect...

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Abstract

The invention discloses a system and a method for optically detecting food-borne pathogenic bacteria, the system comprises a microscope body, the microscope body comprises a base, a support arm, an ocular lens and an objective table, the objective table is provided with a clamping part for clamping a glass slide, the glass slide is provided with a food-borne pathogenic bacteria liquid sample and a nutrient solution, and the objective table is provided with an optical fiber; the microscope body is provided with a first light path communicated with the glass slide and the eyepiece; the system further comprises an illumination assembly for illuminating the glass slide, light passing through the bacterial liquid sample and the nutrient solution forms an optical path difference, the illumination assembly performs optical energy characterization on the food-borne pathogenic bacteria according to the optical path difference and forms a phase difference, and a gray level image is obtained; the hyperspectral imager is used for screening the light passing through the glass slide; the camera shooting assembly is used for acquiring image data passing through the hyperspectral imager; and the characterization module is used for comparing the image data with data in the cell hypercube data to obtain characterization data of the food-borne pathogenic bacteria. The food-borne pathogenic bacteria can be detected by controlling the bright field and the dark field.

Description

technical field [0001] The application belongs to the technical field of high-throughput rapid detection of food-borne pathogens, and in particular relates to a system and method for optically detecting food-borne pathogens. Background technique [0002] Foodborne pathogens are an important cause of food poisoning and foodborne diseases, which seriously threaten the life and health of consumers. How to achieve early and rapid detection of various pathogenic microorganisms is one of the challenges facing global food safety. Food-borne pathogens are usually small in size and use food as a carrier to sneak into the human body to multiply rapidly, causing various diseases such as nausea, vomiting, acute gastroenteritis, bloody diarrhea, kidney or liver failure, and even cancer. At present, the detection of food-borne pathogens in my country is mainly carried out through post-mortem epidemiological investigations, and there is a long detection cycle, which makes it easy to miss t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25
CPCG01N21/25G01N21/255
Inventor 康睿程雅雯周玲莉贡宇陈诚任妮
Owner JIANGSU ACAD OF AGRI SCI