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Method for inducing reverse Malangoni flow based on photo-thermal and method for delivering drug cluster cells

A photothermal induction, cell technology, applied in animal cells, vertebrate cells, tumors/cancer cells, etc., can solve the problems of inactivation, thermal damage, photodamage of biological samples, etc., and achieve the effect of avoiding damage

Active Publication Date: 2022-07-05
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, direct irradiation of biological samples with high-intensity lasers will cause photodamage to biological samples accompanied by thermal damage, and drug-loaded objects may also be inactivated by laser-induced photochemical or photothermal reactions.

Method used

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  • Method for inducing reverse Malangoni flow based on photo-thermal and method for delivering drug cluster cells
  • Method for inducing reverse Malangoni flow based on photo-thermal and method for delivering drug cluster cells
  • Method for inducing reverse Malangoni flow based on photo-thermal and method for delivering drug cluster cells

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preparation example Construction

[0039]The preparation method of the drug-loaded particles of the present invention preferably includes using 1 μm mesoporous silica particles to modify carboxyl groups and load the drug. The drug loaded by the drug-loaded particles of the present invention is preferably determined for the type of the cell, and when the cell is a blood cancer cell or a HeLa cell, the drug includes doxorubicin hydrochloride (DOX). In the present invention, the concentration of the drug-loaded particles is preferably 0.001 mg / ml, and the 1 μm mesoporous silica nanoparticles and DOX are preferably purchased from Xi'an Ruixi Biotechnology Co., Ltd. The method of drug loading is not particularly limited in the present invention, but preferably includes 1 ml of porous silicon spheres modified with carboxyl groups, adding 1 ml of doxorubicin hydrochloride with a concentration of 5 ng / ml, wrapping with tin foil, and placing on a shaker at room temperature for 24 hours. The drug loading method was carri...

Embodiment 1

[0043] Step 1: Preparation of droplets surrounded by silicone oil

[0044] First, drop a small amount of silicone oil (viscosity 5) on the glass substrate placed horizontally, and the silicone oil spreads rapidly on the glass surface to form an oil film with a thickness of about 5 μm. A small amount (5 μL) of aqueous solution was added dropwise to the center of the oil film. Due to the high density of the water droplets, the silicone oil was gradually discharged under the action of gravity. After the water droplet touches the glass surface, it quickly spreads on the glass surface due to the formation of a three-phase contact line. figure 1 Droplets surrounded by silicone oil are shown.

[0045] Step 2: Culture of blood cancer cells and staining of cells with live and dead dyes

[0046] Blood cancer cells were cultured in RPMIMedium 1640 basic (1X) medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin / streptomycin (Pen / strem), and placed at 37°C in 5 %CO 2...

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Abstract

The invention provides a method for inducing reverse Malangoni flow based on photo-thermal and a drug cluster cell delivery method, and relates to the technical field of drug delivery. A silicone oil film is flatly laid on a glass substrate, and liquid drops surrounded by silicone oil are formed after the liquid drops on the film are stable. Wherein the liquid drops contain cells and drug-loaded particles, when laser of optical tweezers is irradiated into silicone oil, stable reverse Malangoni flow can be generated at the boundary of water and oil, so that the drug-loaded particles in the solution are clustered, one or two cells are grabbed by the optical tweezers and placed near the boundary of water and oil, and the drug-loaded particles in the solution are clustered. And the drug cluster cell delivery is controlled by controlling the movement of the light trap. The laser is irradiated into the silicone oil, and the silicone oil has strong absorption at the laser wavelength of the corresponding optical tweezers, so that heat is generated in the silicone oil, and the damage of light and heat to cells is avoided. Reverse Malangoni flow of a photo-thermal induction water-oil interface enables the drug-loading particle cluster to move to the periphery of cells, and drug delivery is accurately completed.

Description

technical field [0001] The invention belongs to the technical field of drug delivery, and in particular relates to a method based on photothermal induced reverse Marangoni flow and a method for drug cluster cell delivery. Background technique [0002] Drug delivery to cells is of great significance for precision medicine. Remarkable progress has been made in drug delivery based on micro / nanomotors with diverse structures and various components based on different motion mechanisms (e.g., optical, chemical, magnetic, acoustic, and biological), but due to complex physiological conditions, micro / nanomotors based on Delivery systems for nanomotor clusters are still limited in biomedical applications. For the delivery of samples such as proteins, nucleic acids, and drugs, strong electric fields, high-intensity light, or direct binding of drugs to magnetic particles in the above-mentioned motor mechanisms will inevitably have irreversible effects on the activity of biological samp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12N5/071C12N13/00
CPCC12N5/0694C12N5/0693C12N5/0682C12N13/00C12N2533/12C12N2533/30C12N2529/10Y02A50/30
Inventor 史阳辛洪宝王丹宁
Owner JINAN UNIVERSITY
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