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Preparation method of graphene electron microscope grid

A technology of graphene and graphene membrane, which is applied in the field of structural biology to achieve the effects of easy acquisition, avoiding structural influence, and high success rate

Pending Publication Date: 2022-07-12
SHUIMU BIOSCIENCES LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] As a two-dimensional carbon nanomaterial, graphene has excellent optical, electrical and mechanical properties, good electrical conductivity, strong mechanical properties, good uniformity and a thickness of only 0.34nm, which can partially or even completely eliminate the current electron microscope grid. Problems such as strong background noise, poor electrical conductivity, and distribution of macromolecules at the gas-liquid interface

Method used

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  • Preparation method of graphene electron microscope grid
  • Preparation method of graphene electron microscope grid
  • Preparation method of graphene electron microscope grid

Examples

Experimental program
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Embodiment

[0079] The present embodiment uses paraffin as the transfer medium to make the graphene electron microscope carrier network, which specifically includes the following steps:

[0080] 1. Removal of graphene on the back: Cut the copper foil covered with graphene grown by chemical vapor deposition (CVD) of suitable size, put the back side up into the Gatan plasma cleaner, and feed oxygen (flow rate 11.5sccm) and argon at the same time (flow rate 35sccm) plasma cleaning was performed for 30 seconds to remove the graphene covered on the backside of the copper foil.

[0081] 2. Waxing: Place the graphene-copper foil structure flat on the cover glass with the front side up, then place the cover glass on a metal bath at 95°C, and take an appropriate amount of paraffin on the graphene surface. The paraffin melts and spreads quickly, covering the graphene surface. Remove the coverslip and leave it at room temperature, the paraffin solidifies rapidly.

[0082] 3. Etch the copper foil: ...

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Abstract

The invention provides a preparation method of a graphene electron microscope grid, which comprises the following steps of: paving a hydrocarbon mixture, namely paving the hydrocarbon mixture on at least part of the surface of a graphene film formed on a metal substrate; a step of transferring the graphene film to an electron microscope grid: removing the metal substrate, and transferring the graphene film paved with the hydrocarbon mixture to the electron microscope grid to obtain a hydrocarbon mixture-graphene film-electron microscope grid structure; and removing the hydrocarbon mixture, namely cleaning the hydrocarbon mixture-graphene film-electron microscope grid structure by adopting an organic solvent to remove the hydrocarbon mixture, thereby obtaining the graphene electron microscope grid.

Description

technical field [0001] The invention belongs to the field of structural biology, and relates to a method for preparing a graphene electron microscope carrier network, which utilizes a hydrocarbon mixture to transfer graphene to the electron microscope carrier network, so as to be applied to cryo-electron microscope sample preparation to obtain a high-resolution biological sample structure. Background technique [0002] In recent years, with the invention of direct electron detection cameras and the development of three-dimensional reconstruction algorithms, single-particle cryo-electron microscopy technology has been able to analyze the structure of biological macromolecules with atomic resolution, which has greatly promoted our understanding of life phenomena at the atomic scale. understanding, and drug development optimization process. However, sample preparation is still one of the main bottlenecks restricting the further development of structural elucidation of biologica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01J37/26H01J37/20C01B32/194C01B32/182
CPCH01J37/261H01J37/20C01B32/182C01B32/194Y02P70/50
Inventor 王宏伟郭春龙刘楠陆叶倪晓丹
Owner SHUIMU BIOSCIENCES LTD
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