Genetic engineering application of wild soybean NADPH oxidase gene GsRbohA1

A wild soybean and genetic engineering technology, applied in the field of genetic engineering, can solve the problem of lack of Rboh gene, etc., and achieve the effect of improving the resistance of Spodoptera litura
CN114774437AActive Publication Date: 2022-07-22NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2022-07-22

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Abstract

The invention discloses an application of a wild soybean NADPH (Nicotinamide Adenine Dinucleotide Phosphate) oxidase gene GsRbohA1. The invention relates to an application of a wild soybean NADPH oxidase gene GsRbohA1 as shown in SEQ ID NO.1 in gene engineering modification of the resistance of soybeans to prodenia litura. The active oxygen content of the soybean hairy roots over-expressed with the GsRbohA1 is increased, and the resistance to prodenia litura is increased. The soybean hairy roots for inhibiting the expression of the gene have no significant change in resistance to prodenia litura. The wild soybean resistance haplotype GsRbohA1A transcripts more GsRbohA1, accumulates more ROS (reactive oxygen species), and has stronger resistance to prodenia litura. According to the present invention, the wild soybean NADPH oxidase gene GsRbohA1 has the potential of introducing the wild soybean resistance haplotype GsRbohA1A into the cultivated soybean so as to improve the resistance of the soybean to the prodenia litura;
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Description

technical field

[0001] The invention relates to the application of wild soybean NADPH oxidase gene GsRbohA1, and belongs to the field of genetic engineering. Background technique

[0002] Soybean is the main oil crop in the world and is widely used in food, feed, industrial production and other side industries. However, soybeans will be threatened by a variety of insect pests during the whole growth period, which will seriously affect the yield of soybeans. Wild soybean is a closely related wild species of cultivated soybean. Although the phenotypic difference between the two is relatively large, there is no reproductive isolation. Wild soybeans live in the wild for a long time and are subject to natural selection. They have strong stress resistance and richer genetic diversity. It is an effective strategy for soybean resistance breeding to mine resistance genes from wild soybean, and transfer the resistance genes of wild soybean into cultivated varieties through genetic e...

Claims

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