Preparation method of composite nanoparticle aluminum adjuvant
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An aluminum adjuvant and nanoparticle technology, applied in the field of preparation of nanocomposite vaccine adjuvant, can solve the problems of lack of ability of aluminum salt adjuvant to induce Th1 type immune response, inability to induce mucosal immune response, etc., and achieve good delivery and immunity. Enhanced, increased yield, good immune response effects
Active Publication Date: 2022-07-29
TAIZHOU UNIV +1
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[0004] The present invention is to solve the problem that the existing aluminum salt adjuvant lacks the ability to induce Th1 type immune response and cannot induce mucosal immune response, and provides a composite nanoparticle The preparation method of aluminum adjuvant
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specific Embodiment approach 1
[0039] Embodiment 1: The preparation method of the composite nanoparticle aluminum adjuvant of this embodiment includes the following steps:
[0040] Step 1: Prepare the soluble aluminum salt solution
[0041] Dissolve the soluble aluminum salt in deionized water, continue stirring at 20°C-50°C for 9 hours, and sterilize at a high temperature of 120°C for 20 minutes; the volume ratio of the mass of the soluble aluminum salt to the deionized water is (0.2~1) g:( 50~200)mL;
[0042] Step 2: Preparation of N-2-hydroxypropyltrimethylammonium chloride chitosan (N-2-HACC) with controllable degree of substitution
[0043]Dissolve chitosan in acetic acid solution, adjust pH to alkaline, filter with suction, wash with deionized water, freeze-dry, then disperse chitosan in isopropanol solution, add 2,3 glycidyl triglyceride dropwise The isopropanol solution of methyl ammonium chloride, after the dropwise addition, stir in a constant temperature oil bath at 70-90°C for 9-10h, then cool...
specific Embodiment approach 2
[0050] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the soluble aluminum salt in step 1 is one or a mixture of any of aluminum chloride, aluminum sulfate, aluminum acetate, and aluminum nitrate. Others are the same as the first embodiment.
specific Embodiment approach 3
[0051] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the volume ratio of acetic acid to deionized water in the acetic acid solution in step 2 is (8-10):(150-300). Others are the same as in the first or second embodiment.
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Abstract
The invention discloses a preparation method of a composite nanoparticle aluminum adjuvant, and relates to the technical field of vaccine adjuvants, in particular to a preparation method of a nano composite vaccine adjuvant. The invention aims to solve the problems that the existing aluminum salt adjuvant lacks the capability of inducing Th1 type immune response and cannot induce mucosal immune response. The method comprises the following steps: 1, preparing a soluble aluminum salt solution; and 2, preparing the N-2-hydroxypropyl trimethyl ammonium chloride chitosan with a controllable substitution degree. 3, dissolving the N-2-HACC prepared in the step 2 in a sodium acetate buffer solution, and stirring to prepare an N-2-HACC solution; 4, adding the anionic polymer solution into the N-2-HACC solution, and uniformly stirring to obtain a mixed solution; and 5, preparing the N-2-HACC-Al composite nanoparticle aluminum adjuvant. According to the method, the yield can be improved on the premise of controlling the particle size, and the yield is greatly increased. The positively charged and stable nanoparticles exhibit good delivery and immunological enhancement. The preparation method is used for preparing the aluminum adjuvant for the vaccine.
Description
technical field [0001] The invention relates to the technical field of vaccine adjuvants, in particular to a preparation method of a nanocomposite vaccine adjuvant. Background technique [0002] An adjuvant is a substance that is injected into the body prior to or mixed with the antigen at the same time, which can non-specifically enhance the immunogenicity of the antigen or change the type of immune response, but has no immunogenicity itself and cannot cause an immune response. The role of adjuvants in immunization is mainly to enhance the immune response of the body's immune system to antigens or immunogens, including enhancing the strength of the immune response and the persistence of the response. [0003] Aluminum adjuvant plays a certain role in the body's innate immunity, acquired immunity and the response of the complement system. A large number of research and application results show that aluminum adjuvant can promote the differentiation of macrophages and inflamm...
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