Melatonin nerve conduit and preparation method thereof

Abstract

The invention relates to the field of biological materials, in particular to a melatonin nerve conduit and a preparation method thereof. The invention provides a preparation method of a melatonin nerve conduit. The preparation method comprises the following steps: preparing a biodegradable material and a melatonin suspension or adding melatonin into a molten biodegradable material, and uniformly mixing to obtain a mixture; and adding the suspension or the mixture into 3D printing or electrostatic spinning equipment, and carrying out printing or electrostatic spinning to obtain the melatonin nerve conduit. The melatonin nerve conduit prepared by the preparation method provided by the invention has an ideal biomedical functional material. Rat sciatic nerve defect repair experiments show that the melatonin nerve conduit can promote nerve regeneration, improve the maturity of regenerated nerves and the number of nerve fibers which play a role, and promote sciatic nerve functions to recover to normal, and the melatonin nerve conduit has an effect better than that of autologous nerve transplantation repair, and has a good application prospect.

Description

[0001] This application is a divisional application for an invention patent with an application date of August 14, 2018, an application number of 201810921130.7, and an invention title of "A Melatonin Nerve Catheter Composition, Nerve Catheter and Its Preparation Method and Application". technical field [0002] The invention relates to the field of biological materials, in particular to a melatonin nerve conduit and a preparation method thereof. Background technique [0003] Various biomedical catheters, including degradable and non-degradable catheters, such as polyethylene tubes, have been used as nerve catheters to study nerve regeneration. Tissue fibrosis, causing inflammation and other toxic side effects, the clinical application effect is not very ideal. At present, various biodegradable materials have been developed to prepare various biomedical catheters, such as collagen, polylactic acid, etc. However, due to the use of these materials alone, there are also some pr...

AI Technical Summary

Problems solved by technology

[0005] Although some research progress has been made on nerve guides, such as the Chinese invention patent CN100479785C which discloses a method for preparing nerve guides, there is still a certain distance from autologous nerve repair.

In addition, there are many patents disclosing different preparation methods (such as Chinese invention patent CN101439205A, open date 2009.5.27; CN101507842A, open date 2009.8.19, CN106668938A, open date 2017.05.17; CN106924820A, open date 2017.07.07), But these catheters either have issues with strength, toxicity, quality control or cost

Chinese patent 2010800025691 discloses a method and equipment for preparing a three-dimensional porous tubular scaffold. It specifically discloses that the 3D tubular scaffold can be made of non-biodegradable polymers, biodegradable polymers or combinations thereof. Biodegradable polymers include, for example , polylactic acid (PLA), polyglycolic acid (PGA), etc., 3D tubular scaffolds can be added with hormones, examples of hormones include melatonin, etc., 3D tubular scaffolds can also be used as peripheral nerve conduits to guide damaged nerve regeneration; Although the patent discloses the use of melatonin and polylactic acid to prepare nerve conduits, it does not disclose the role of melatonin in promoting nerve repair

None of the nerve guides disclosed in the prior art are ideal biomedical guides

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