Nanoparticles for resisting H1N1 subtype influenza A virus based on self-assembled ferritin as well as preparation method and application of nanoparticles
An influenza virus and nanoparticle technology, applied in the field of genetic engineering, can solve the problem that antibodies cannot provide cross-protection, achieve high sensitivity and curative effect, improve accuracy and repeatability, and shorten the treatment process
Pending Publication Date: 2022-07-29
QINGDAO AGRI UNIV
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The invention discloses nanoparticles for resisting H1N1 subtype influenza A virus based on self-assembled ferritin as well as a preparation method and application of the nanoparticles, and relates to the technical field of genetic engineering. The nanoparticle is formed by connecting a signal peptide-ST-FE fusion protein and a signal peptide-SC-HA-VHH fusion protein in vitro, the signal peptide-ST-FE fusion protein is obtained by connecting a signal peptide-SpyTag as shown in SEQ ID NO: 3 to the N end of a ferritin monomer subunit; the signal peptide-SC-HA-VHH fusion protein is obtained by connecting the end N of a nano antibody of an anti-H1N1 subtype influenza A virus hemagglutinin protein with a signal peptide-SpyCatcher as shown in SEQ ID NO: 4. The invention further discloses a preparation method of the signal peptide-SC-HA-VHH fusion protein. The nanoparticles for resisting the influenza A (H1N1) subtype virus, disclosed by the invention, have great significance in monitoring, preventing and treating the influenza A (H1N1) subtype virus.
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