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Method for detecting concentration of heavy metals in water body

A technology of heavy metals and heavy metal ions, applied in the field of biological monitoring, to achieve the effect of strong portability, simple design, and simple detection steps

Active Publication Date: 2022-07-29
HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, the use of biosensors to detect heavy metals still requires the use of large-scale instruments such as fluorescence spectrophotometers for auxiliary analysis, which has certain limitations in practical environments.

Method used

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  • Method for detecting concentration of heavy metals in water body
  • Method for detecting concentration of heavy metals in water body
  • Method for detecting concentration of heavy metals in water body

Examples

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Effect test

Embodiment 1

[0042] A method for detecting the concentration of heavy metals in a water body, the method for detecting the concentration of heavy metals in a water body comprises the following steps:

[0043] S1. Inoculate the genetically engineered bacteria XG210 in the culture medium to obtain OD 600 = 0.2 bacterial liquid; the bacterial liquid was dispensed into a 96-well plate, the volume of each well of bacterial liquid was 96 μL, cultured at 37 °C for 10 h, and then 4 μL of deionized water was added, and 4 μL of concentration were 50 μM, 100 μM, 150 μM, 200 μM, 250 μM cadmium ion solution, so that the cadmium ion concentration gradient in the final bacterial solution is 0 μM, 2 μM, 4 μM, 6 μM, 8 μM, 10 μM, each concentration is set 5 replicates, and incubated at 37 ° C for 30 min to obtain a mixed solution;

[0044] A 6cm×6cm polytetrafluoroethylene film (0.3mm thick) is sanded with sandpaper to a hydrophobic angle of 110° to obtain a specific polytetrafluoroethylene film;

[0045] ...

Embodiment 2

[0049] A method for detecting the concentration of heavy metals in a water body, the method for detecting the concentration of heavy metals in a water body comprises the following steps:

[0050] S1. genetically engineered bacteria are inoculated in the culture medium to obtain OD 600 = 0.3 bacterial liquid; the bacterial liquid was dispensed into a 96-well plate, the volume of each well of bacterial liquid was 96 μL, cultured at 37 ° C for 12 h, and then 4 μL of deionized water was added, and 4 μL of concentration were respectively 50 μM, 100 μM, 150 μM, 200 μM, 250 μM cadmium ion solution, so that the cadmium ion concentration gradient in the final bacterial solution is 0 μM, 2 μM, 4 μM, 6 μM, 8 μM, 10 μM, each concentration is set 5 replicates, and incubated at 37 ° C for 60 min to obtain a mixed solution;

[0051] Use sandpaper to polish a 8cm×8cm PTFE film (0.5mm thick) to a hydrophobic angle of 150° to obtain a specific PTFE film;

[0052] S2. Drop 6 μL of the mixture o...

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Abstract

The invention belongs to the technical field of biological monitoring, and particularly relates to a method for detecting the concentration of heavy metals in a water body, which comprises the following steps: S1, preparing a genetically engineered bacterium solution, then adding a to-be-detected solution containing heavy metal ions into the bacterium solution, and culturing to obtain a mixed solution; preparing a modified polytetrafluoroethylene film; s2, dropping the mixed solution on the modified polytetrafluoroethylene film, detecting the fluorescence intensity and analyzing the concentration of heavy metal ions; wherein the genetically engineered bacterium contains a transcription regulation protein with heavy metal specific response, a promoter specifically regulated by the transcription regulation protein and a characteristic protein presenting red fluorescence; and the hydrophobic angle of the specific polytetrafluoroethylene film is 110-150 degrees. The method can effectively realize accurate and rapid detection of heavy metals in a complex environment, and has great significance in guaranteeing the safety of a water environment and accelerating the treatment process of water environment pollution.

Description

technical field [0001] The invention belongs to the technical field of biological monitoring, and particularly relates to a method for detecting the concentration of heavy metals in a water body. Background technique [0002] With economic development and population increase, water resources are becoming increasingly scarce, and continuous pollution problems have led to continuous deterioration of the water environment. Water pollution incidents due to wastewater discharge, leakage of toxic pollutants, poor water management or monitoring, etc. are emerging one after another, resulting in increasingly serious water safety problems. Heavy metals, microplastics, antibiotics, personal care products, etc. are all pollutants of major concern for water quality safety. Among them, heavy metal ions are widely concerned because of their high toxicity, non-biodegradability and persistence, and are easily spread through the food chain, leading to bioaccumulation and threatening the hea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12Q1/6897G01N33/18C12R1/63
CPCC12Q1/025C12Q1/6897G01N33/1866G01N2333/28Y02A20/20
Inventor 路璐高翔李涵皮姗姗杨睿洁杨文君
Owner HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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