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Method for extracting castor silk fibroin by using modified block copolymer and calcium chloride

A technology of block copolymer and silk fibroin, which is applied in the field of protein extraction, can solve the problems of high toxicity, great harm to human body, and does not conform to green health, etc., and achieves the effects of mild working conditions, resource saving and easy recovery.

Pending Publication Date: 2022-08-02
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common lignan solvents are highly toxic and harmful to the human body, which does not conform to the concept of green and healthy

Method used

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  • Method for extracting castor silk fibroin by using modified block copolymer and calcium chloride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Weigh 4.0g of castor cocoons and cut them open, place them in a clean beaker, wash away surface contaminants with ultrapure water, and dry.

[0027] 2) Weigh 0.14g of modified block copolymer, Na 2 CO 3 0.14g, Na 2 HPO 4 0.14 g, dissolved in 9 mL of ultrapure water, and sonicated at 30°C for 15 min to obtain a mixed solution. The castor silk was boiled for 35 min in the mixed solution at a liquor ratio of 1:100, and degummed for three times in total. The modified block copolymer is a block copolymer formed by the copolymerization of methyl propylene oxide and propylene oxide, followed by adding fatty alcohol polyoxyethylene ether; methyl propylene oxide, propylene oxide and fatty alcohol polyoxyethylene The molar ratio of vinyl ether was 3.2:1.1:0.6.

[0028] 3) After degumming, the castor silk was washed with ultrapure water, then rinsed with absolute ethanol, washed three times, and placed in a 60° C. oven to obtain dry silk fibroin fibers.

[0029] 4) Degu...

Embodiment 2

[0032] 1) Weigh 5.0g of castor cocoons and cut them open, place them in a clean beaker, wash off surface contaminants with ultrapure water, and dry.

[0033] 2) Weigh 0.15g of modified block copolymer, Na 2 CO 3 0.15g, Na 2 HPO 4 0.15 g, dissolved in 10 mL of ultrapure water, and sonicated at 30 °C for 15 min to obtain a mixed solution. The castor silk was boiled in the mixed solution at a liquor ratio of 1:98 for 38 min, and degummed for three times in total. The modified block copolymer is a block copolymer formed by the copolymerization of methyl propylene oxide and propylene oxide, followed by adding fatty alcohol polyoxyethylene ether; methyl propylene oxide, propylene oxide and fatty alcohol polyoxyethylene The molar ratio of vinyl ether was 3.6:1.3:0.7.

[0034] 3) After degumming, the castor silk was washed with ultrapure water, then rinsed with absolute ethanol, washed three times, and placed in a 62° C. oven to obtain dry silk fibroin fibers.

[0035] 4) Degu...

Embodiment 3

[0038] 1) Weigh 4.5g of castor cocoons and cut them open, place them in a clean beaker, wash off surface contaminants with ultrapure water, and dry.

[0039] 2) Weigh 0.16g of modified block copolymer, Na 2 CO 3 0.16g, Na 2 HPO 4 0.16 g, dissolved in 11 mL of ultrapure water, and sonicated at 30°C for 15 min to obtain a mixed solution. The castor silk was boiled in the mixed solution for 40 min at a liquor ratio of 1:95, and degummed for three times in total. The modified block copolymer is a block copolymer formed by the copolymerization of methyl propylene oxide and propylene oxide, followed by adding fatty alcohol polyoxyethylene ether; methyl propylene oxide, propylene oxide and fatty alcohol polyoxyethylene The molar ratio of vinyl ether was 3.6:1.7:0.8.

[0040] 3) After degumming, the castor silk was washed with ultrapure water, then rinsed with absolute ethanol, washed three times, and placed in a 65° C. oven to obtain dry silk fibroin fibers.

[0041] 4) Degum...

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Abstract

The invention relates to the field of protein extraction, and discloses a method for extracting silk fibroin of castor silk by using a modified block copolymer and calcium chloride. The modified block copolymer is used for dissolving lignan on the surface of the castor silk to obtain pure fibroin fibers which are convenient for subsequent dissolution and extraction by using a calcium chloride solution; the preparation method comprises the following steps: adding absolute ethyl alcohol into degummed silk fibroin to remove impurities, dissolving obtained silk fibroin, and dialyzing and freeze-drying to obtain silk fibroin powder. The extraction method disclosed by the invention has the characteristics of high safety coefficient, small harm to a human body, greenness and health, and the modified block copolymer has a very good dissolving effect on lignan, so that the problem that castor silk is difficult to extract is solved.

Description

technical field [0001] The invention relates to the field of protein extraction, in particular to a method for extracting castor silk fibroin by using a modified block copolymer and calcium chloride. Background technique [0002] Silk is an animal protein fiber material with a very wide range of applications and a long history of use. Its main component is silk fibroin, accounting for more than 70% of the total protein content. Silk fibroin itself has excellent mechanical and physical and chemical properties, such as flexibility, Tensile strength and other properties are relatively good, and different forms can be obtained after different treatments, such as fibers, solutions, powders, films and gels, etc. However, natural silk fibroin contains a large number of intramolecular and intermolecular hydrogen bonds, crystallinity It is relatively high and difficult to dissolve in general solvents. In this respect, the castor silkworm is more than the silkworm. [0003] At presen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/36C07K1/34C07K1/30C07K1/14
CPCC07K14/43563
Inventor 胡铭周王秉刘锋徐冰王毓彭志勤万军民
Owner ZHEJIANG SCI-TECH UNIV
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