Hydrogel microneedle patch for noninvasive blood glucose detection as well as preparation method and application of hydrogel microneedle patch
A blood sugar detection and hydrogel technology, which is applied in the field of biomedical research, can solve the problems of skin irritation, seldom, pain, etc., and achieve the effects of rapid response, simple preparation steps, and easy process conditions.
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[0044] 1. Preparation of pH-responsive hydrogel CMC-pHEA
[0045] First, put 50ml of distilled water and carboxymethyl cellulose (CarboxyMethy1 Cellulose, CMC) (5.56×10mol) into a 250m1 three-neck round-bottom flask at 75°C (50-100°C, preferably 75°C) and 400rpm (300- 500rpm, preferably 400rpm) in a water bath with stirring until the CMC is fully dissolved. Then nitrogen was injected into the partial pressure bottle containing the CMC solution for 20 minutes to fully reduce the oxygen content in the solution, and then potassium persulfate (KPS, 1.85×105mol) was added to the solution as an initiator to mix the reaction for 20 minute. Next, 3.92×10 mol of 2-hydroxyethyl acrylate (2-HydroxyEthy1 Acrylate, 2-HEA) was added. When the reaction mixture became milky white, 0.32×10mol crosslinking agent polyethylene glycol diacrylate PEGDA was added, and the reaction was continued for 3 hours. After the reaction was completed, it was fully cooled at room temperature. The stirring sp...
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