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Process for industrial production of oligochitose and chitooligose

A production method and low-chitosan technology, applied in the direction of oligosaccharides, etc., can solve the problems of inability to degrade chitosan, and achieve the effect of feasible production process, low production cost, and cheap and easy-to-obtain raw materials

Inactive Publication Date: 2004-12-01
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem that the present invention needs to solve is to provide a kind of method that adopts complex enzyme to degrade chitosan and obtain low chitosan and chitosan oligosaccharide, to overcome the existing technology that cannot make chitosan degrade to lower molecular weight Chitooligosaccharide Defects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Get 10 kilograms of chitosan and be dissolved in 240L concentration and be in the acetic acid solution of 1%, form 250 kilograms of 4% colloid solution, add 100 gram compound enzymes (lipase: amylase=1: 5, this lipase and amylase select (product from Wuxi Genencor Bioengineering Co., Ltd.), react at 50°C for 3 hours, add sodium hydroxide to neutralize to alkaline, freeze to -18°C, thaw and centrifuge, vacuum dry at 50°C, and pulverize to obtain an average molecular weight of 35000 9 kg of low shell sugar.

[0019] project

Embodiment 2

[0021] Get 5 kilograms of chitosan and be dissolved in 240L concentration and be in the acetic acid solution of 1%, form 250 kilograms of colloid solution, add 100 gram compound enzymes (lipase: amylase=1: 10, this lipase and amylase select Products from Wuxi Genencor Bioengineering Co., Ltd.), react at 50°C for 3 hours, heat up to 95°C for 15 minutes, cool, filter, membrane ultrafiltration with a molecular weight cut-off of about 10,000, nanofiltration, freeze-drying, crushing , Obtaining average molecular weight is 4 kilograms of chitosan oligosaccharides of 1000.

[0022] project

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PUM

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Abstract

An industrial process for preparing oligochitose or chitoligose by degradating chitosan with complex enzyme includes specifically opening the beta (1,4) glycoside bond of chitosan with complex enzyme, separating out oligothitose in alkaline condition, freezing, centrifugal dewatering, vacuum drying, superfiltering for removing enzyme, nano-filtering for dewatering and freeze drying. Its advantages are high quality of product and low cost.

Description

technical field [0001] The invention relates to the industrial production technology of low chitosan and chitosan oligosaccharides, in particular to a method for degrading chitosan under the action of non-specific compound enzymes and separating and purifying to obtain oligosaccharides or chitosan oligosaccharides. Background technique [0002] Chitosan is the deacetylation product of chitin. Chitin widely exists in crustaceans such as shrimps and crabs, as well as insects, algae and bacteria. It is the second largest natural polymer compound after cellulose in the world, and its annual biosynthesis amount reaches billions of tons. Due to its inactive chemical properties and poor solubility, chitin has limited direct application; however, the chitosan obtained after deacetylation of chitin has poor solubility, The chemical properties have been greatly changed, and it has a wide range of application values ​​in food, medicine, daily chemicals, environmental protection, agric...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H3/06
Inventor 金鑫荣张文清
Owner EAST CHINA UNIV OF SCI & TECH
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